防御素β1(DEFb1)重组蛋白
Recombinant Defensin Beta 1 (DEFb1)
B-DF1; DEFB1; BD1; DEF-B1; DEFB101; HBD1
- 编号RPB373Bo01
- 物种Bos taurus; Bovine (Cattle,牛) 相同的名称,不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位细胞膜, 分泌
- 预测分子量18.0kDa
- 实际分子量22kDa(差异分析请参阅说明书)
- 片段与标签Asp1~Trp38 with Two N-terminal Tags, His-tag and SUMO-tag
- 缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
- 性状冻干粉
- 纯度> 80%
- 等电点8.6
-
应用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,请参见活性蛋白。 - 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1152 ¥ 2880 ¥ 5760 ¥ 17280 ¥ 43200
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Bos taurus; Bovine (Cattle,牛) | 应用(仅供研究使用,不用于临床诊断!) |
RPB373Bo01 | 防御素β1(DEFb1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB373Bo01 | 防御素β1(DEFb1)多克隆抗体 | WB; IHC; ICC; IP. |
MAB373Bo21 | 防御素β1(DEFb1)单克隆抗体 | WB; IHC; ICC; IP. |
SEB373Bo | 防御素β1(DEFb1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB373Bo | 防御素β1(DEFb1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
Appl Environ Microbiol. | Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell Line [Asm: Source] |
British Journal of Nutrition | Identification of sociodemographic and clinical factors associated with the levels of human β-defensin-1 and human β-defensin-2 in the human milk of Han Chinese [Pubmed: 24124699] |
International Journal of Dairy Technology | Concentration characteristics of bovine β-defensin 1 and 2 in fresh bovine milk and infant formula [Doi: 10.1111] |
Molecular Nutrition Food Research | Green tea polyphenol epigallocatechin-3-gallate improves epithelial barrier function by inducing the productionof antimicrobial peptide pBD-1 and pBD-2 in monolayers of porcine intestinal epithelial IPEC-J2 cells. [Pubmed:26991948] |
Frontiers in Microbiology | Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense [Pubmed:29997590] |
International Immunopharmacology | Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase … [Pubmed: 30342347] |
cyberleninka | EFFECT OF NIOSOMAL ANTIMICROBIAL PEPTIDE HBD-1 ON THE HEALING RATE OF INFECTED WOUNDS IN RATS [] |
Fundamental & clinical pharmacology | Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivo [Pubmed: 31313350] |
Cytokine | Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis [Pubmed: 31927460] |
Animals | Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport [33375218] |
Sci Rep | Human beta defensin levels and vaginal microbiome composition in post-menopausal women diagnosed with lichen sclerosus [34362937] |
J Mol Histol | Isolation, culture, and identification of ceruminous gland cells [Pubmed:35113280] |
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