中性粒细胞弹性蛋白酶(NE)重组蛋白
Recombinant Neutrophil Elastase (NE)
ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Leukocyte Elastase; PMN Elastase
- 编号RPA181Hu03
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位分泌
- 预测分子量37.0kDa
- 实际分子量40kDa(差异分析请参阅说明书)
- 片段与标签Ile30~Gln247 with Two N-terminal Tags, His-tag and SUMO-tag
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 80%
- 等电点8.7
-
应用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,请参见活性蛋白。 - 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 725 ¥ 1812 ¥ 3624 ¥ 10872 ¥ 27180
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPA181Hu02 | 中性粒细胞弹性蛋白酶(NE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA181Hu03 | 中性粒细胞弹性蛋白酶(NE)活性蛋白 | Cell culture; Activity Assays. |
RPA181Hu01 | 中性粒细胞弹性蛋白酶(NE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA181Hu03 | 中性粒细胞弹性蛋白酶(NE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA181Hu04 | 中性粒细胞弹性蛋白酶(NE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA181Hu01 | 中性粒细胞弹性蛋白酶(NE)活性蛋白 | Cell culture; Activity Assays. |
PAA181Hu01 | 中性粒细胞弹性蛋白酶(NE)多克隆抗体 | WB; IHC; ICC; IP. |
MAA181Hu22 | 中性粒细胞弹性蛋白酶(NE)单克隆抗体 | WB; IHC; ICC; IP. |
SEA181Hu | 中性粒细胞弹性蛋白酶(NE)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA181Hu | 中性粒细胞弹性蛋白酶(NE)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA181Hu | 中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
Mediators of Inflammation | Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation [Hindawi: 612848] |
Journal of Photochemistry and Photobiology B: Biology | A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins [Pubmed:25732262] |
International Journal of Molecular Medicine | The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats [pubmed:27430552] |
European Journal of Trauma and Emergency | Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in acanine burn model. [pubmed:28070608] |
Clin Exp Pharmacol Physiol. | Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. [pubmed:28251688] |
Journal of Cardiology | Targeted regulation of sympathetic activity in paraventricular nucleus reduces inducible ventricular arrhythmias in rats after myocardial infarction [10.1016:j.jjcc.2018.06.003] |
Neuroscience | Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure Rats [Pubmed:29684507] |
Molecular and Cellular Biochemistry | Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytes [Pubmed:28948423] |
Neuropsychiatric disease and treatment | The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic … [Pubmed:29379286] |
Behavioural Brain Research | Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats [Pubmed: 30423388] |
Journal of Pineal Research | Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice [Pubmed: 30929267] |
Fundamental & clinical pharmacology | Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivo [Pubmed: 31313350] |
Cell Death & Disease | Erythromycin suppresses neutrophil extracellular traps in smoking-related chronic pulmonary inflammation [Pubmed: 31515489] |
Journal of nutritional science and vitaminology | Co-Administration of Curcumin and Artepillin C Induces Development of Brown-Like Adipocytes in Association with Local Norepinephrine Production by Alternatively … [Pubmed: 31474682] |
Journal of Clinical Immunology | MiR-608 Exerts Anti-inflammatory Effects by Targeting ELANE in Monocytes [Pubmed: 31749032] |
Pathology & Oncology Research | Neutrophil Extracellular Traps Associate with Clinical Stages in Breast Cancer [Pubmed: 31656990] |
Bioscience Reports | Inhibition of microRNA-146a attenuated heart failure in myocardial infarction rats [Pubmed: 31763669] |
Oxidative Medicine and Cellular Longevity | Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation [Pubmed: 31871555] |
Cytokine | Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis [Pubmed: 31927460] |
Journal of neural transmission (Vienna, Austria : 1996) | Neurotransmitter system aberrations in patients with drug addiction [Pubmed: 32804296] |
experimental eye research | Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitis [Pubmed: 32376471] |
CLINICAL AND EXPERIMENTAL HYPERTENSION | Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats [Pubmed: 32349626] |
Br J Pharmacol | Macrophage Extracellular Traps Aggravate Iron Overload©\Related Liver Ischemia/Reperfusion Injury [33959955] |
Evidence-Based Complementary and Alternative Medicine | Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling?¡ [33505502] |
Nat Sci Sleep | Depression of Non-Neuronal Cholinergic System May Play a Role in Co-Occurrence of Subjective Daytime Sleepiness and Hypertension in Patients with … [34934375] |
Neuromodulation: Technology at the Neural Interface | Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome [Pubmed:35088760] |
A Novel Role of Tinospora Cordifolia in Amelioration of Cancer-Induced Systemic Deterioration By Taming Neutrophil Infiltration and Hyperactivation [] |
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