拓扑异构酶Ⅰ(TOP1)重组蛋白
Recombinant Topoisomerase I (TOP1)
TOPI; DNA Topoisomerase 1
- 编号RPC243Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位细胞核
- 预测分子量33.9kDa
- 实际分子量38kDa(差异分析请参阅说明书)
- 片段与标签Met1~Ser250 with N-terminal His Tag
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 80%
- 等电点9.4
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应用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，请参见活性蛋白。 - 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1080 ¥ 2700 ¥ 5400 ¥ 16200 ¥ 40500
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC243Hu01 | 拓扑异构酶Ⅰ(TOP1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC243Hu01 | 拓扑异构酶Ⅰ(TOP1)多克隆抗体 | WB; IHC; ICC; IP. |
| MAC243Hu22 | 拓扑异构酶Ⅰ(TOP1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEC243Hu | 拓扑异构酶Ⅰ(TOP1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC243Hu | 拓扑异构酶Ⅰ(TOP1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Electronic Journal of Biotechnology | Comparing the expression of human DNA topoisomerase I in KM71H and X33 strains of Pichia pastoris [science:S0717345816000233] |
| Journal of Experimental & Clinical Cancer Research | Inhibitory effects of lapachol on rat C6 glioma in vitro and in vivo by targeting DNA topoisomerase I and topoisomerase II [pubmed:27852319] |
| Tikrit Journal of Pure Science | Partial purification of topoisomerase IB from serum of diabetic patients and study it's kinetic properties and molecular weight [] |
| EurAsian Journal of BioSciences | Study of topoisomerase I (Topo I) level and some biochemical parameters in diabetic patients [] |
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