补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法,宽范围)
Wide-range ELISA Kit for Complement Component 3a (C3a)
- 编号WEA387Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.156-10ng/mL
- 灵敏度最小可检测剂量小于等于0.064ng/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2516 ¥ 3594 ¥ 16173 ¥ 30549 ¥ 251580
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法,宽范围)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法,宽范围)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 101 |
| EDTA plasma(n=5) | 81-103 | 87 |
| heparin plasma(n=5) | 93-104 | 99 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法,宽范围)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法,宽范围)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-95% | 98-105% | 95-104% | 78-94% |
| EDTA plasma(n=5) | 97-105% | 94-103% | 92-105% | 80-97% |
| heparin plasma(n=5) | 83-97% | 83-101% | 90-103% | 79-103% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法,宽范围)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法,宽范围)与连接于固相载体上的抗体结合，然后加入生物素化的补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法,宽范围)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法,宽范围)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA387Hu01 | 补体成分3a(C3a)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA387Hu01 | 补体成分3a(C3a)活性蛋白 | Cell culture; Activity Assays. |
| PAA387Hu01 | 补体成分3a(C3a)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA387Hu27 | 补体成分3a(C3a)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA387Hu21 | 补体成分3a(C3a)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA387Hu22 | 补体成分3a(C3a)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA387Hu23 | 补体成分3a(C3a)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA387Hu24 | 补体成分3a(C3a)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA387Hu29 | 补体成分3a(C3a)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA387Hu25 | 补体成分3a(C3a)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA387Hu | 补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEA387Hu | 补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA387Hu | 补体成分3a(C3a)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA387Hu | 补体成分3a(C3a)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA387Hu01 | 补体成分3a(C3a)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
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| Journal of Antimicrobial Chemotherapy | Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model [Pubmed: 23645587] |
| SpringerPlus | Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats [Pubmed: 24353977] |
| Gynecol Obstet Invest | Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? [PubMed: 26485247] |
| Am J Reprod Immunol | Human IgG Antinuclear Antibodies Induce Pregnancy Loss in Mice by Increasing Immune Complex Deposition in Placental Tissue: In Vivo Study [PubMed: 26388133] |
| Journal of Neuroimmunology | Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis [Pubmed:27235358] |
| Plos One | Alternative Pathway Inhibition by Exogenous Factor H Fails to Attenuate Inflammation and Vascular Leakage in Experimental Pneumococcal Sepsis in Mice [Pubmed:26872035] |
| Digital Repository | Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos [10183] |
| Colloids Surf B Biointerfaces. | Interaction of gold and silver nanoparticles with human plasma: Analysis of protein corona reveals specific binding patterns. [pubmed:28131092] |
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| Journal of Immunology | The Complement C3a–C3aR Axis Promotes Development of Thoracic Aortic Dissection via Regulation of MMP2 Expression [Pubmed:29367209] |
| Artificial Cells Nanomedicine and Biotechnology Journal | Long term safety and immunological effects of a nanobiotherapeutic, bovine poly-[hemoglobin-catalase-superoxide dismutase-carbonic anhydrase], after four weekly … [Pubmed:29873524] |
| Cytotherapy | Mesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantation [Doi: 10.1016/j.jcyt.2018.07.007] |
| Journal of NeuroVirology | Intrathecal complement activation by the classical pathway in tick-borne encephalitis [Pubmed: 30850976] |
| Clinical Immunology | Pathogenic roles of anti-C1q antibodies in recurrent pregnancy loss [] |
| Materials Science and Engineering: C | Hemocompatibility of biodegradable Zn-0.8 wt%(Cu, Mn, Li) alloys [] |
| Nanomedicine | Complement-mediated hypersensitivity reactions to an amphotericin B-containing lipid complex (Abelcet) in pediatric patients and anesthetized rats: Benefits of slow infusion [33549818] |
| EXPERIMENTAL EYE RESEARCH | Increased ratios of complement factors C3a to C3 in aqueous humor and serum mark glaucoma progression [33493474] |
| FASEB J | C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease [Pubmed:35503088] |
