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α1-酸性糖蛋白(a1AGP)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Alpha-1-Acid Glycoprotein (a1AGP)

AGP1; a1-AGP; ORM1; OMD 1; Orosomucoid 1

  • α1-酸性糖蛋白(a1AGP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • α1-酸性糖蛋白(a1AGP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对α1-酸性糖蛋白(a1AGP)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPA816Mu01 α1-酸性糖蛋白(a1AGP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA816Mu02 α1-酸性糖蛋白(a1AGP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA816Mu01 α1-酸性糖蛋白(a1AGP)多克隆抗体 WB,IHC
PAA816Mu02 α1-酸性糖蛋白(a1AGP)多克隆抗体 WB; IHC; ICC; IP.
LAA816Mu81 α1-酸性糖蛋白(a1AGP)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA816Mu71 α1-酸性糖蛋白(a1AGP)多克隆抗体(生物素标记) WB; IHC; ICC.
SEA816Mu α1-酸性糖蛋白(a1AGP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA816Mu α1-酸性糖蛋白(a1AGP)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA816Mu01 α1-酸性糖蛋白(a1AGP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
J Reprod Dev.? An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In Vitro [Pubmed:Pmc4219990]
Molecular Reproduction and Development Evidence for a novel, local acute-phase response in the bovine oviduct: Progesterone and lipopolysaccharide up-regulate alpha 1-acid-glycoprotein expression in epithelial cells in vitro [Pubmed:25123565]
BMC Complementary and Alternative Medicine Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study [Pubmed:25652121]
PLoS One Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study [PubMed: 25955845]
Sci Rep.  S100A9 and ORM1 serve as predictors of therapeutic response and prognostic factors in advanced extranodal NK/T cell lymphoma patients treated with pegaspargase/gemcitabine [pmc:PMC4810364]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
Scientific Reports An autoregressive logistic model to predict the reciprocal effects of oviductal fluid components on in vitro spermophagy by neutrophils in cattle [pubmed:28667317]
33 Transcriptome Analysis Uncovers a Growth-Promoting Activity of Orosomucoid-1 on Hepatocytes. [pubmed:28927749]
Brazilian Journal of Otorhinolaryngology Inflammatory markers in palatine tonsils of children with obstructive sleep apnea syndrome [Pubmed: 30213594]
BioRxiv Proteome-wide characterization and biomarker identification of intracranial aneurysm []
The FEBS journal Dramatically changed immune‐related molecules as early diagnostic biomarkers of non‐small cell lung cancer [Pubmed: 31482685]
PLoS One Effects of vaccination on acute-phase protein response in broiler chicken [Pubmed: 32191767]
Indian Journal of Animal Sciences Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy … []
A New Biomarker Tool for Risk Stratification in ¡°De Novo¡± Acute Heart Failure (OROME) []
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