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降钙素基因相关肽(CGRP)活性蛋白

Active Calcitonin Gene Related Peptide (CGRP)

CALCB; CGRP2; CALC2; CGRP-II; Calcitonin Gene Related Peptide 2; Calcitonin 2; Calcitonin Related Polypeptide Beta

  • 降钙素基因相关肽(CGRP)活性蛋白 产品包装(模拟)
  • 降钙素基因相关肽(CGRP)活性蛋白 产品包装(模拟)
  • APA876Ra01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Calcitonin gene-related peptide (CGRP) is a highly potent vasoactive peptide released from sensory nerves. CGRP is a 37-amino acid and it has two major forms (α and β). It belongs to a group of peptides that all act on an unusual receptor family. These receptors consist of calcitonin receptor-like receptor (CLR) linked to an essential receptor activity modifying protein (RAMP) that is necessary for full functionality. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat CGRP and recombinant human RAMP2. Briefly, CGRP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to RAMP2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CGRP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat CGRP and recombinant human RAMP2 was shown in Figure 1, the EC50 for this effect is 0.06 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
APA876Ra01 降钙素基因相关肽(CGRP)活性蛋白 Cell culture; Activity Assays.
RPA876Ra01 降钙素基因相关肽(CGRP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA876Ra21 降钙素基因相关肽(CGRP)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA876Ra01 降钙素基因相关肽(CGRP)多克隆抗体 WB
MAA876Ra21 降钙素基因相关肽(CGRP)单克隆抗体 WB; IHC; ICC; IP.
CEA876Ra 降钙素基因相关肽(CGRP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA876Ra 降钙素基因相关肽(CGRP)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA876Ra 降钙素基因相关肽(CGRP)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA876Ra11 降钙素基因相关肽(CGRP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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PLoS One. Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid A [Pubmed:Pmc4028234]
Int J Vasc Med. Prognostic significance of circulating and endothelial progenitor cell markers in type 2 diabetic foot. [Pubmed:24624298]
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Pain OnabotulinumtoxinA decreases interictal CGRP plasma levels in patients with chronic migraine [PubMed: 25735000]
Molecular Medicine Reports Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats [PubMed: 25892548]
Annals of Allergy, Asthma and Immunology Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis [Pubmed:27156748]
Cephalalgia. Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients [Pubmed:27250233]
Cardiology.  Transient Receptor Potential Vanilloid Subtype 1 Inhibits Inflammation and Apoptosis via the Release of Calcitonin Gene-Related Peptide in the Heart after Myocardial Infarction. [Pubmed:27144592]
J Headache Pain Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-кB [Pubmed:27150105]
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9 Probiotic Bifidobacterium longum NCC3001 Reduces Depression Scores and Alters Brain Activity: A Pilot Study in Patients With Irritable Bowel Syndrome [pubmed:28483500]
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BioRxiv - the preprint server for biology RAMP1 in Kupffer cells is a critical regulator in immune-mediated hepatitis [10.1101:357582]
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Molecular and Cellular Biochemistry Rhynchophylline attenuates migraine in trigeminal nucleus caudalis in nitroglycerin-induced rat model by inhibiting MAPK/NF-кB signaling [Pubmed: 31420791]
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Respiratory Physiology & Neurobiology Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma [Pubmed: 31542455]
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Scientific Reports Mild systemic inflammation enhances response to OnabotulinumtoxinA in chronic migraineurs [33441852]
Nature Communications Perirenal adipose afferent nerves sustain pathological high blood pressure in rats [Pubmed:35668093]
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