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降钙素基因相关肽(CGRP)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Calcitonin Gene Related Peptide (CGRP)

CALCB; CGRP2; CALC2; CGRP-II; Calcitonin Gene Related Peptide 2; Calcitonin 2; Calcitonin Related Polypeptide Beta

  • 降钙素基因相关肽(CGRP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 降钙素基因相关肽(CGRP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 降钙素基因相关肽(CGRP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品图片
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对降钙素基因相关肽(CGRP)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
APA876Ra01 降钙素基因相关肽(CGRP)活性蛋白 Cell culture; Activity Assays.
RPA876Ra01 降钙素基因相关肽(CGRP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA876Ra21 降钙素基因相关肽(CGRP)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA876Ra01 降钙素基因相关肽(CGRP)多克隆抗体 WB
MAA876Ra21 降钙素基因相关肽(CGRP)单克隆抗体 WB; IHC; ICC; IP.
CEA876Ra 降钙素基因相关肽(CGRP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA876Ra 降钙素基因相关肽(CGRP)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA876Ra 降钙素基因相关肽(CGRP)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA876Ra11 降钙素基因相关肽(CGRP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Evidence-Based Complementary and Alternative Medicine Alstonia scholaris R. Br. Significantly Inhibits Retinoid-Induced Skin Irritation In Vitro and In Vivo. [Hindawi: 190370]
The Journal of Headache and Pain Increased levels of CGRP in peripheral blood in women with chronic migraine: A reliable biological marker [PubMed: PMC3620076]
Headache: The Journal of Head and Face Pain CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine [Onlinelibrary: head.12372]
PLoS One. Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid A [Pubmed:Pmc4028234]
Int J Vasc Med. Prognostic significance of circulating and endothelial progenitor cell markers in type 2 diabetic foot. [Pubmed:24624298]
Arch Gynecol Obstet. Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control study [Pubmed:24943060]
Pain OnabotulinumtoxinA decreases interictal CGRP plasma levels in patients with chronic migraine [PubMed: 25735000]
Molecular Medicine Reports Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats [PubMed: 25892548]
Annals of Allergy, Asthma and Immunology Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis [Pubmed:27156748]
Cephalalgia. Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients [Pubmed:27250233]
Cardiology.  Transient Receptor Potential Vanilloid Subtype 1 Inhibits Inflammation and Apoptosis via the Release of Calcitonin Gene-Related Peptide in the Heart after Myocardial Infarction. [Pubmed:27144592]
J Headache Pain Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-кB [Pubmed:27150105]
Frontiers in Physiology Prevention of Bone Loss in a Model of Postmenopausal Osteoporosis throughAdrenomedullin Inhibition. [pubmed:27445864]
Naunyn Schmiedebergs Arch Pharmacol. Opioid receptor agonists may favorably affect bone mechanical properties in rats with estrogen deficiency-induced osteoporosis. [pubmed:27896372]
American Journal of Translational Research A new comorbidity model and the common pathological mechanisms of migraine and epilepsy [pubmed:28559979]
9 Probiotic Bifidobacterium longum NCC3001 Reduces Depression Scores and Alters Brain Activity: A Pilot Study in Patients With Irritable Bowel Syndrome [pubmed:28483500]
Journal of Clinical Investigation CNS myeloid cells critically regulate heat hyperalgesia [Pubmed:29634489]
Journal of Headache and Pain Feasibility of serum CGRP measurement as a biomarker of chronic migraine: a critical reappraisal [Pubmed:30006780]
BioRxiv - the preprint server for biology RAMP1 in Kupffer cells is a critical regulator in immune-mediated hepatitis [10.1101:357582]
International Journal of Pharmaceutical Sciences and Research MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL []
JOURNAL OF PERIODONTOLOGY Periodontal inflammation is related to increased serum calcitonin gene‐related peptide (CGRP) levels in patients with chronic migraine [Pubmed: 31070784]
Molecular and Cellular Biochemistry Rhynchophylline attenuates migraine in trigeminal nucleus caudalis in nitroglycerin-induced rat model by inhibiting MAPK/NF-кB signaling [Pubmed: 31420791]
Journal of Headache and Pain Electrical stimulation of the superior sagittal sinus suppresses A-type K+ currents and increases P/Q-and T-type Ca 2+ currents in rat trigeminal ganglion … []
Respiratory Physiology & Neurobiology Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma [Pubmed: 31542455]
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