碳酸酐酶Ⅱ(CA2)活性蛋白
Active Carbonic Anhydrase II (CA2)
CA-II; CAII; Car2; CAC; Carbonate dehydratase II; Carbonic anhydrase C
- 编号APA782Ra01
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 97%
- 等电点6.9
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 840 ¥ 2100 ¥ 4200 ¥ 12600 ¥ 31500
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产品包装(模拟)
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产品包装(模拟)
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SDS-PAGE图
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通过ISO 9001、ISO 13485质量体系认证
活性实验
CA2 (Carbonic anhydrase 2) is an enzyme that catalyzes reversible hydration of carbon dioxide. It is essential for bone resorption and osteoclast differentiation and contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. It is widely accepted that CA2 also catalyzes hydrolysis of p-Nitrophenyl Acetate. Thus, a hydration assay was conducted to test the catalytic activity of CA2 using 4-Nitrophenyl Acetate (4-NPA) as substrate. Briefly, different concentrations of CA2 were incubated with 1mM 4-NPA in reaction buffer. The absorbance at the wavelength of 400nm was read per hour, and the result was shown in figure 1. It is obvious that CA2 catalyzes hydrolysis of p-Nitrophenyl Acetate.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| NPA782Ra01 | 碳酸酐酶Ⅱ(CA2)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA782Ra02 | 碳酸酐酶Ⅱ(CA2)活性蛋白 | Cell culture; Activity Assays. |
| APA782Ra01 | 碳酸酐酶Ⅱ(CA2)活性蛋白 | Cell culture; Activity Assays. |
| RPA782Ra01 | 碳酸酐酶Ⅱ(CA2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA782Ra02 | 碳酸酐酶Ⅱ(CA2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA782Ra01 | 碳酸酐酶Ⅱ(CA2)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA782Ra71 | 碳酸酐酶Ⅱ(CA2)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA782Ra | 碳酸酐酶Ⅱ(CA2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA782Ra | 碳酸酐酶Ⅱ(CA2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA782Ra01 | 碳酸酐酶Ⅱ(CA2)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Proteomics Clin Appl. | Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two-dimensional nano-liquid chromatography/nanoelectrospray ionization-mass spectrometry. [Pubmed:26970563] |
| PROTEOMICS - Clinical Applications | Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. [pubmed:26680509] |
| Cell & Molecular Biology | Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two‐dimensional nano‐liquid chromatography/nanoelectrospray … [doi:10.1002] |