碳酸酐酶Ⅱ(CA2)活性蛋白
Active Carbonic Anhydrase II (CA2)
CA-II; CAII; Car2; CAC; Carbonate dehydratase II; Carbonic anhydrase C
- 编号APA782Ra02
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 95%
- 等电点6.8
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. The activity of recombinant rat CA2 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of CA2 (diluted by assay buffer) to 50 µL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37℃ for 5min, then read at a wavelength of 400 nm.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| NPA782Ra01 | 碳酸酐酶Ⅱ(CA2)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA782Ra02 | 碳酸酐酶Ⅱ(CA2)活性蛋白 | Cell culture; Activity Assays. |
| APA782Ra01 | 碳酸酐酶Ⅱ(CA2)活性蛋白 | Cell culture; Activity Assays. |
| RPA782Ra01 | 碳酸酐酶Ⅱ(CA2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA782Ra02 | 碳酸酐酶Ⅱ(CA2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA782Ra01 | 碳酸酐酶Ⅱ(CA2)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA782Ra71 | 碳酸酐酶Ⅱ(CA2)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA782Ra | 碳酸酐酶Ⅱ(CA2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA782Ra | 碳酸酐酶Ⅱ(CA2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA782Ra01 | 碳酸酐酶Ⅱ(CA2)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Proteomics Clin Appl. | Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two-dimensional nano-liquid chromatography/nanoelectrospray ionization-mass spectrometry. [Pubmed:26970563] |
| PROTEOMICS - Clinical Applications | Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. [pubmed:26680509] |
| Cell & Molecular Biology | Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two‐dimensional nano‐liquid chromatography/nanoelectrospray … [doi:10.1002] |