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胱天蛋白酶1(CASP1)活性蛋白

Active Caspase 1 (CASP1)

ICE; IL1BC; IL1-BC; P45; Interleukin 1 Beta Converting Enzyme; Apoptosis-Related Cysteine Peptidase; Interleukin-1 beta convertase

  • 胱天蛋白酶1(CASP1)活性蛋白 产品包装(模拟)
  • 胱天蛋白酶1(CASP1)活性蛋白 产品包装(模拟)
  • APB592Ra01.jpg SDS-PAGE图
  • 胱天蛋白酶1(CASP1)活性蛋白 Figure. Western Blot; Sample: Recombinant CASP1, Rat.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Caspase 1 (CASP1) cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. It also has lots of other function, such as defenses against pathogens, cleaves and activates sterol regulatory element binding proteins (SREBPs), promotes apoptosis. Besides, Interleukin 1 Alpha (IL1a) has been identified as an interactor of CASP1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CASP1 and recombinant rat IL1a. Briefly, CASP1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1a-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CASP1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of CASP1 and IL1a was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
APB592Ra01 胱天蛋白酶1(CASP1)活性蛋白 Cell culture; Activity Assays.
RPB592Ra01 胱天蛋白酶1(CASP1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB592Ra01 胱天蛋白酶1(CASP1)多克隆抗体 WB; IHC; ICC; IP.
LAB592Ra71 胱天蛋白酶1(CASP1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB592Ra21 胱天蛋白酶1(CASP1)单克隆抗体 WB; IHC; ICC; IP.
SEB592Ra 胱天蛋白酶1(CASP1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB592Ra 胱天蛋白酶1(CASP1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
PLoS One. Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion [Pubmed:24816434]
Fertil Steril Inflammosome in the human endometrium: further step in the evaluation of the “maternal side” [PubMed: 26474737]
PLoS One Synthetic PreImplantation Factor (PIF) prevents fetal loss by modulating LPS induced inflammatory response. [pubmed:28704412]
Journal of Virology NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitination [Pubmed:29386291]
Journal of Translational Medicine Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation? [Pubmed:29665864]
Reproductive Sciences A role for the inflammasome in spontaneous labor at term [Pubmed:27852921]
Journal of Virology CHARACTERIZATION OF PORCINE NLRP3 INFLAMMASOME-MEDIATED INTERLEUKIN-1 BETA PRODUCTION IN RESPONSE TO INFLUENZA A VIRUSES [Pubmed: 29386291]
ACTA Otorhinolaryngologica Italica NLRP3 inflammasome expression in lipopolysaccharide-induced otitis media [Pubmed: 30676848]
American Journal of Reproductive Immunology Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss women [Pubmed: 31148259]
Taiwanese Journal of Obstetrics & Gynecology Serum Caspase-1 levels in women with polycystic ovary syndrome [Pubmed: 32127139]
Iranian Journal of Allergy, Asthma and Immunology Increased Level of Caspase-1 in the Serum of Relapsing-remitting Multiple Sclerosis (RRMS) Patients []
ACTIVITY OF APOPTOSIS MARKERS IN HPV-ASSOCIATED CERVICAL PATHOLOGIES []
Norwegian Journal of development of the International Science CLINICAL AND PATHOGENETIC SIGNIFICANCE OF CHANGES IN PURINE METABOLISM DURING LUNG CANCER []
LIFE SCIENCES The NLRP3 inhibitor dapansutrile attenuates folic acid induced nephrotoxicity via inhibiting inflammasome/caspase-1/IL axis and regulating autophagy/proliferation [34560082]
Biol Reprod The alarmin S100A12 causes sterile inflammation of the human chorioamniotic membranes and preterm birth and neonatal mortality in mice [34632484]
J Mol Neurosci TRPV1 Suppressed NLRP3 Through Regulating Autophagy in Microglia After Ischemia-Reperfusion Injury [Pubmed:35041191]
Viruses Influenza A Virus Infection Activates NLRP3 Inflammasome through Trans-Golgi Network Dispersion [Pubmed:35062292]
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