表皮生长因子(EGF)活性蛋白
Active Epidermal Growth Factor (EGF)
URG; Beta-Urogastrone; Pro-epidermal growth factor
- 编号APA560Hu04
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点4.6
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 暂无报价 暂无报价 暂无报价 暂无报价 暂无报价
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Epidermal growth factor (EGF) is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. To test the effect of EGF on cell proliferation of 3T3 fibroblasts, Balb/c 3T3 cells were seeded into triplicate wells of 96-well plates and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant human EGF. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate，then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 °C. Cell proliferation of Balb/c 3T3 cells after incubation with rhEGF for 48h observed by inverted microscope was shown in Figure 1. The dose-effect curve of rhEGF was shown in Figure2. It was obvious that rhEGF significantly promoted cell proliferation of 3T3 cells. The ED50 for this effect is typically 3.9 ng/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA560Hu02 | 表皮生长因子(EGF)活性蛋白 | Cell culture; Activity Assays. |
| APA560Hu06 | 表皮生长因子(EGF)活性蛋白 | Cell culture; Activity Assays. |
| RPA560Hu03 | 表皮生长因子(EGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA560Hu04 | 表皮生长因子(EGF)活性蛋白 | Cell culture; Activity Assays. |
| RPA560Hu08 | 表皮生长因子(EGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA560Hu09 | 表皮生长因子(EGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA560Hu02 | 表皮生长因子(EGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA560Hu06 | 表皮生长因子(EGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA560Hu01 | 表皮生长因子(EGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA560Hu01 | 表皮生长因子(EGF)活性蛋白 | Cell culture; Activity Assays. |
| PAA560Hu05 | 表皮生长因子(EGF)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA560Hu01 | 表皮生长因子(EGF)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA560Hu02 | 表皮生长因子(EGF)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA560Hu71 | 表皮生长因子(EGF)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA560Hu22 | 表皮生长因子(EGF)单克隆抗体 | WB; IHC |
| MAA560Hu24 | 表皮生长因子(EGF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA560Hu23 | 表皮生长因子(EGF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA560Hu25 | 表皮生长因子(EGF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA560Hu27 | 表皮生长因子(EGF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA560Hu21 | 表皮生长因子(EGF)单克隆抗体 | WB; IHC; ICC; IP. |
| HEA560Hu | 表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA560Hu | 表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEA560Hu | 表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA560Hu | 表皮生长因子(EGF)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA560Hu | 表皮生长因子(EGF)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSA560Hu01 | 表皮生长因子(EGF)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA560Hu01 | 表皮生长因子(EGF)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Journal of Pediatric Surgery | The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model [PubMed: 20438940] |
| Biomark Insights | Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism [PubMed: PMC3623607] |
| Plos one | The Probiotic Mixture VSL#3 Accelerates Gastric Ulcer Healing by Stimulating Vascular Endothelial Growth Factor [PubMed: PMC3590171] |
| Diabetes. | Allogeneic transplantation of an adipose-derived stem cell (ASC) sheet combined with artificial skin accelerates wound healing in a rat wound model of type 2 diabetes and obesity [Pubmed:25795216] |
| Eur Rev Med Pharmacol Sci.? | Association of colon adenomas and skin tags: coincidence or coexistence? [Pubmed:24763889] |
| Int J Clin Exp Med. | The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell [PubMed: 26628972] |
| ACS Applied Materials & Interfaces | Polydopamine nanoparticles modulating stimuli-responsive PNIPAM hydrogels with cell/tissue adhesiveness. [pubmed:27709887] |
| Canadian Journal of Physiology and Pharmacology | The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats [pubmed:28177680] |
| Journal of Controlled Release | A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury [pubmed:28522195] |
| Scientific Reports | Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets [pubmed:28811665] |
| International Journal of Nanomedicine | Micro-/nano-topography of selective laser melting titanium enhances adhesion and proliferation and regulates adhesion-related gene expressions of human … [Pubmed: 30233172] |
| Neoplasia | Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine [Pubmed: 33212364] |
| Aesthet Surg J | Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water [33428732] |
| Cell Death & Disease | Sodium/glucose cotransporter 1-dependent metabolic alterations induce tamoxifen resistance in breast cancer by promoting macrophage M2 polarization [34006822] |
| Stem Cells International | The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin?¡ [33381190] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … [Pubmed:35585377] |
| Biomaterials Advances | Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats [] |
| Authorea | Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy [] |
| Biointerface Research in Applied Chemistry | Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats [] |
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