太阳集团tyc5997_平台(china)官方网站

中脑星形胶质细胞来源神经营养因子(MANF)活性蛋白

Active Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF)

ARMET; ARP; Arginine Rich Mutated In Early Stage Tumors; Arginine-rich protein

  • 中脑星形胶质细胞来源神经营养因子(MANF)活性蛋白 产品包装(模拟)
  • 中脑星形胶质细胞来源神经营养因子(MANF)活性蛋白 产品包装(模拟)
  • 中脑星形胶质细胞来源神经营养因子(MANF)活性蛋白 Figure. Gene Sequencing (Extract)
  • APC300Hu01.png Figure. SDS-PAGE
  • 中脑星形胶质细胞来源神经营养因子(MANF)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

MANF (Mesencephalic astrocyte-derived neurotrophic factor) is a prosurvival protein that protects the cells when applied intracellularly in vitro or extracellularly in vivo. Recently, MANF has also been proven to inhibit cell proliferation and ER stress-induced cell death and also affects cell size and morphology under certain conditions. Thus the bioactivity of human recombinant MANF were detected using U87-MG. Briefly, U87-MG cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of MANF. After incubated for 48h, cells were observed by inverted microscope and were shown in Figure 1. It was obvious that MANF inhibited cell proliferation of U87-MG and affected cell size and morphology. (A)U7-MG cells cultured in DMEM, stimulated with 0.01ng/mL MANF for 48h;
(B)Unstimulated U87-MG cells cultured in DMEM for 48h.
Figure 1. Inhibition of U87-MG cell proliferation after stimulated with MANF.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
APC300Hu61 中脑星形胶质细胞来源神经营养因子(MANF)活性蛋白 Cell culture; Activity Assays.
EPC300Hu61 中脑星形胶质细胞来源神经营养因子(MANF)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC300Hu01 中脑星形胶质细胞来源神经营养因子(MANF)活性蛋白 Cell culture; Activity Assays.
RPC300Hu01 中脑星形胶质细胞来源神经营养因子(MANF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC300Hu01 中脑星形胶质细胞来源神经营养因子(MANF)多克隆抗体 WB; IHC; ICC; IP.
MAC300Hu25 中脑星形胶质细胞来源神经营养因子(MANF)单克隆抗体 WB
MAC300Hu21 中脑星形胶质细胞来源神经营养因子(MANF)单克隆抗体 WB; IHC; ICC; IP.
MAC300Hu23 中脑星形胶质细胞来源神经营养因子(MANF)单克隆抗体 WB; IHC; ICC; IP.
MAC300Hu24 中脑星形胶质细胞来源神经营养因子(MANF)单克隆抗体 WB; IHC; ICC; IP.
MAC300Hu27 中脑星形胶质细胞来源神经营养因子(MANF)单克隆抗体 WB; IHC; ICC; IP.
MAC300Hu22 中脑星形胶质细胞来源神经营养因子(MANF)单克隆抗体 WB,IHC
MEC300Hu 中脑星形胶质细胞来源神经营养因子(MANF)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SEC300Hu 中脑星形胶质细胞来源神经营养因子(MANF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC300Hu 中脑星形胶质细胞来源神经营养因子(MANF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSC300Hu01 中脑星形胶质细胞来源神经营养因子(MANF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It(ELISA Kit) Yourself"

参考文献

杂志 参考文献
PLoS One. Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome Sequencing [PubMed: PMC3378547]
Endocr J. Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance. [pubmed:28216543]
Journal of Steroid Biochemistry and Molecular Biology Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling [pubmed:28263910]
Endocrine Journal Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance [10.1507:endocrj.EJ16-0472]
Nature Metabolism MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage []
J Exp Med Feeding-induced hepatokine, Manf, ameliorates diet-induced obesity by promoting adipose browning via p38 MAPK pathway [33856409]
J Inflamm Res The Potential Protective Effect of Mesencephalic Astrocyte-Derived Neurotrophic Factor on Post-Operative Delirium via Inhibiting Inflammation and Microglia … [34234505]
留言咨询
Baidu
sogou