中脑星形胶质细胞来源神经营养因子(MANF)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF)
ARMET; ARP; Arginine Rich Mutated In Early Stage Tumors; Arginine-rich protein
- 编号KSC300Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围15.6-1,000pg/mL
- 可适灵敏度6.7pg/mL
- 应用Main materials for "Do It(ELISA Kit) Yourself"
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7560 ¥ 12600 ¥ 22680 ¥ 44100 ¥ 75600
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对中脑星形胶质细胞来源神经营养因子(MANF)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APC300Hu61 | 中脑星形胶质细胞来源神经营养因子(MANF)活性蛋白 | Cell culture; Activity Assays. |
| EPC300Hu61 | 中脑星形胶质细胞来源神经营养因子(MANF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC300Hu01 | 中脑星形胶质细胞来源神经营养因子(MANF)活性蛋白 | Cell culture; Activity Assays. |
| RPC300Hu01 | 中脑星形胶质细胞来源神经营养因子(MANF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC300Hu01 | 中脑星形胶质细胞来源神经营养因子(MANF)多克隆抗体 | WB; IHC; ICC; IP. |
| MAC300Hu25 | 中脑星形胶质细胞来源神经营养因子(MANF)单克隆抗体 | WB |
| MAC300Hu21 | 中脑星形胶质细胞来源神经营养因子(MANF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC300Hu23 | 中脑星形胶质细胞来源神经营养因子(MANF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC300Hu24 | 中脑星形胶质细胞来源神经营养因子(MANF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC300Hu27 | 中脑星形胶质细胞来源神经营养因子(MANF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC300Hu22 | 中脑星形胶质细胞来源神经营养因子(MANF)单克隆抗体 | WB,IHC |
| MEC300Hu | 中脑星形胶质细胞来源神经营养因子(MANF)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEC300Hu | 中脑星形胶质细胞来源神经营养因子(MANF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC300Hu | 中脑星形胶质细胞来源神经营养因子(MANF)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSC300Hu01 | 中脑星形胶质细胞来源神经营养因子(MANF)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It(ELISA Kit) Yourself" |
参考文献
| 杂志 | 参考文献 |
| PLoS One. | Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome Sequencing [PubMed: PMC3378547] |
| Endocr J. | Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance. [pubmed:28216543] |
| Journal of Steroid Biochemistry and Molecular Biology | Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling [pubmed:28263910] |
| Endocrine Journal | Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance [10.1507:endocrj.EJ16-0472] |
| Nature Metabolism | MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage [] |
| J Exp Med | Feeding-induced hepatokine, Manf, ameliorates diet-induced obesity by promoting adipose browning via p38 MAPK pathway [33856409] |
| J Inflamm Res | The Potential Protective Effect of Mesencephalic Astrocyte-Derived Neurotrophic Factor on Post-Operative Delirium via Inhibiting Inflammation and Microglia … [34234505] |
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