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胰高血糖素(GCG)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Glucagon (GCG)

GC; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone

  • 胰高血糖素(GCG)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 胰高血糖素(GCG)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对胰高血糖素(GC)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

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参考文献

杂志 参考文献
Molecular Metabolism PER2 promotes glucose storage to liver glycogen during feeding and acute fasting by inducing Gys2 PTG and GL expression [PubMed: PMC3773838]
Exp Biol Med (Maywood). Increasing glucagon secretion could antagonize the action of exogenous insulin for glycemic control in streptozocin-induced diabetic rhesus monkeys. [Pubmed: 23760004]
European Journal of Endocrinology Specificity and sensitivity of commercially available assays for glucagon and oxyntomodulin measurement in human [Eje-online: Source]
American Diabetes Association IL-21 is a major negative regulator of IRF4-dependent lipolysis affecting Tregs in adipose tissue and systemic insulin sensitivity [Pubmed:24430438]
Journal of Pharmacy and Pharmacology Enzogenol improves diabetes-related metabolic change in C57BL/KsJ-db/db?mice, a model of type 2 diabetes mellitus [Pubmed:24611864]
European Journal of Pharmacology Subcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse model [Pubmed:27131827]
Appl Physiol Nutr Metab The glucoreCavia (Guinea pig )latory response to high-intensity aerobic exercise following training in rats with insulin-treated type 1 diabetes mellitus [Pubmed:27175938]
American Journal of Translational Research Subcutaneous liraglutide ameliorates methylglyoxal-induced Alzheimer-like tau pathology and cognitive impairment by modulating tau hyperphosphorylation and glycogen synthase kinase-3β. [pubmed:28337257]
Biomedicine & Pharmacotherapy Vitamin D3 intake as regulator of insulin degrading enzyme and insulin receptor phosphorylationin diabetic rats. [pubmed:27930980]
Diabetologia Ginsenoside Rg5 attenuates hepatic glucagon response via suppression of succinate-associated HIF-1α induction in HFD-fed mice [pubmed:28280902]
International Journal of Molecular Sciences Effects of Acute Cold Stress on Liver O-GlcNAcylation and Glycometabolism in Mice [Pubmed: 30231545]
European Journal of Pharmacology Exogenous obestatin decreases beta-cell apoptosis and alfa-cell proliferation in high fat diet and streptozotocin induced type 2 diabetic rats [Pubmed: 30776368]
Stress Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposure [Pubmed: 30821572]
international journal of molecular sciences Effects of Cold-inducible RNA-binding Protein (CIRP) on Liver Glycolysis during Acute Cold Exposure in C57BL/6 Mice [Pubmed: 30909542]
Diabetes-Metabolism Research and Reviews Liraglutide Combined with Human Umbilical Cord Mesenchymal Stem Cell Transplantation inhibits beta‐cells apoptosis via mediating the ASK1/JNK/BAX pathway in [Pubmed: 31411368]
CELL DISCOVERY In vitro expansion of pancreatic islet clusters facilitated by hormones and chemicals [Pubmed: 32284878]
endocrine journal Mice with nucleus tractus solitarius injury induced by chronic restraint stress present impaired ability to raise blood glucose and glucagon levels when blood glucose … [Pubmed: 32249244]
Frontiers in Endocrinology RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis [Pubmed: 32328034]
Molecular Medicine Reports Acarbose ameliorates spontaneous type‑2 diabetes in db/db mice by inhibiting PDX‑1 methylation [Pubmed: 33236139]
CHEMICO-BIOLOGICAL INTERACTIONS Modulatory role of imatinib mesylate on pancreatic β-cells' secretory functions in an STZ rat model of diabetes mellitus [Pubmed: 32710900]
Mol Cell Endocrinol Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells [34391847]
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