胰高血糖素(GCG)重组蛋白
Recombinant Glucagon (GCG)
GC; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone
- 编号RPB266Ra01
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位分泌
- 预测分子量22.2kDa
- 实际分子量25kDa(差异分析请参阅说明书)
- 片段与标签His21~Lys180 with N-terminal His Tag
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性状冻干粉
- 纯度> 97%
- 等电点6.1
-
应用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,请参见活性蛋白。 - 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1003 ¥ 2508 ¥ 5016 ¥ 15048 ¥ 37620
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPB266Ra01 | 胰高血糖素(GCG)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB266Ra01 | 胰高血糖素(GCG)多克隆抗体 | WB; IHC; ICC; IP. |
MAB266Ra21 | 胰高血糖素(GCG)单克隆抗体 | WB; IHC; ICC; IP. |
CEB266Ra | 胰高血糖素(GCG)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB266Ra | 胰高血糖素(GCG)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB266Ra11 | 胰高血糖素(GCG)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Molecular Metabolism | PER2 promotes glucose storage to liver glycogen during feeding and acute fasting by inducing Gys2 PTG and GL expression [PubMed: PMC3773838] |
Exp Biol Med (Maywood). | Increasing glucagon secretion could antagonize the action of exogenous insulin for glycemic control in streptozocin-induced diabetic rhesus monkeys. [Pubmed: 23760004] |
European Journal of Endocrinology | Specificity and sensitivity of commercially available assays for glucagon and oxyntomodulin measurement in human [Eje-online: Source] |
American Diabetes Association | IL-21 is a major negative regulator of IRF4-dependent lipolysis affecting Tregs in adipose tissue and systemic insulin sensitivity [Pubmed:24430438] |
Journal of Pharmacy and Pharmacology | Enzogenol improves diabetes-related metabolic change in C57BL/KsJ-db/db?mice, a model of type 2 diabetes mellitus [Pubmed:24611864] |
European Journal of Pharmacology | Subcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse model [Pubmed:27131827] |
Appl Physiol Nutr Metab | The glucoreCavia (Guinea pig )latory response to high-intensity aerobic exercise following training in rats with insulin-treated type 1 diabetes mellitus [Pubmed:27175938] |
American Journal of Translational Research | Subcutaneous liraglutide ameliorates methylglyoxal-induced Alzheimer-like tau pathology and cognitive impairment by modulating tau hyperphosphorylation and glycogen synthase kinase-3β. [pubmed:28337257] |
Biomedicine & Pharmacotherapy | Vitamin D3 intake as regulator of insulin degrading enzyme and insulin receptor phosphorylationin diabetic rats. [pubmed:27930980] |
Diabetologia | Ginsenoside Rg5 attenuates hepatic glucagon response via suppression of succinate-associated HIF-1α induction in HFD-fed mice [pubmed:28280902] |
International Journal of Molecular Sciences | Effects of Acute Cold Stress on Liver O-GlcNAcylation and Glycometabolism in Mice [Pubmed: 30231545] |
European Journal of Pharmacology | Exogenous obestatin decreases beta-cell apoptosis and alfa-cell proliferation in high fat diet and streptozotocin induced type 2 diabetic rats [Pubmed: 30776368] |
Stress | Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposure [Pubmed: 30821572] |
international journal of molecular sciences | Effects of Cold-inducible RNA-binding Protein (CIRP) on Liver Glycolysis during Acute Cold Exposure in C57BL/6 Mice [Pubmed: 30909542] |
Diabetes-Metabolism Research and Reviews | Liraglutide Combined with Human Umbilical Cord Mesenchymal Stem Cell Transplantation inhibits beta‐cells apoptosis via mediating the ASK1/JNK/BAX pathway in [Pubmed: 31411368] |
CELL DISCOVERY | In vitro expansion of pancreatic islet clusters facilitated by hormones and chemicals [Pubmed: 32284878] |
endocrine journal | Mice with nucleus tractus solitarius injury induced by chronic restraint stress present impaired ability to raise blood glucose and glucagon levels when blood glucose … [Pubmed: 32249244] |
Frontiers in Endocrinology | RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis [Pubmed: 32328034] |
Molecular Medicine Reports | Acarbose ameliorates spontaneous type‑2 diabetes in db/db mice by inhibiting PDX‑1 methylation [Pubmed: 33236139] |
CHEMICO-BIOLOGICAL INTERACTIONS | Modulatory role of imatinib mesylate on pancreatic β-cells' secretory functions in an STZ rat model of diabetes mellitus [Pubmed: 32710900] |
Mol Cell Endocrinol | Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells [34391847] |
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