谷胱甘肽(GSH)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Glutathione (GSH)
L-Reduced Glutathione
- 编号KSA294Ge11
- 物种Pan-species (General,通用) 相同的名称,不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围1.23-100ug/mL
- 可适灵敏度0.48ug/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 8597 ¥ 14328 ¥ 25790 ¥ 50148 ¥ 85968
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对谷胱甘肽(GSH)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Pan-species (General,通用) | 应用(仅供研究使用,不用于临床诊断!) |
SPA294Ge01 | 谷胱甘肽(GSH)合成多肽 | Immunogen; Blocking Peptide. |
CPA294Ge11 | 谷胱甘肽(GSH)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA294Ge21 | 谷胱甘肽(GSH)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA294Ge41 | 谷胱甘肽(GSH)人血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
PAA294Ge01 | 谷胱甘肽(GSH)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
LAA294Ge71 | 谷胱甘肽(GSH)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAA294Ge22 | 谷胱甘肽(GSH)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA294Ge21 | 谷胱甘肽(GSH)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
CEA294Ge | 谷胱甘肽(GSH)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA294Ge | 谷胱甘肽(GSH)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA294Ge11 | 谷胱甘肽(GSH)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
European Journal of Clinical Nutrition (2012) 66 | Evidence for augmented oxidative stress in the subjects with type 1 diabetes and their siblings: a possible preventive role for antioxidants [PubMed: 22781023] |
International Journal of Medical Sciences | Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma [PubMed: PMC3619117] |
Reproduction | Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis [Pubmed:25376627] |
Iran J Basic Med Sci | Effects of mild hypothermia therapy on the levels of glutathione in rabbit blood and cerebrospinal fluid after cardiopulmonary resuscitation [PubMed: 25810895] |
Bulletin of the Veterinary Institute in Pulawy | Oxidative Stability of Ostrich Meat Related to Duration of Linseed and Lucerne Supplementation to the Bird's Diet [View: J] |
Journal of Allergy and Clinical Immunology | Prenatal maternal distress affects atopic dermatitis in offspring mediated by oxidative stress [Pubmed:27016803] |
Oxidative Medicine and Cellular Longevity | Hydrogen sulfide protects against chronic unpredictable mild stress-induced oxidative stress in hippocampus by upreCavia (Guinea pig )lation of BDNF-TrkB pathway [journals:2153745.pdf] |
Institutional repository of Vilnius University | Rapid opioid detoxification with an implementation of the new method of gradually increasing dosage of naltrexone induction [object:elaba:15733325] |
Molecular Medicine Reports | Toxicity study of oxalicumone A, derived from a marine-derived fungus Penicillium oxalicum, in cultured renal epithelial cells [pubmed:28260084] |
Molecular Medicine Reports | Hepatocellular toxicity of oxalicumone A via oxidative stress injury and mitochondrial dysfunction in healthy human liver cells [pubmed:29115483] |
Cancer Medicine | Metabolomic and transcriptomic profiling of hepatocellular carcinomas in Hras12V transgenic mice [pubmed:28941178] |
Free radical research | Circulating cell-free DNA of methylated insulin-like growth factor-binding protein 7 predicts a poor prognosis in hepatitis B virus-associated hepatocellular carcinoma … [Pubmed:29463155] |
Biomed Research International | Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway [Pubmed:29770336] |
Molecular Medicine Reports | Epigallocatechingallate attenuates myocardial injury in a mouse model of heart failure through TGF‑β1/Smad3 signaling pathway [Pubmed:29620209] |
Experimental and Therapeutic Medicine | Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway [Pubmed:30116359] |
Journal of Integrative Medicine | Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats [Doi: 10.1016/j.joim.2018.12.002] |
EXPERIMENTAL ANIMALS | Protective effects of sulforaphane on diabetic retinopathy: activation of the Nrf2 pathway and inhibition of NLRP3 inflammasome formation [Pubmed: 30606939] |
Acta Biomaterialia | Porous Se@ SiO2 nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species … [Pubmed: 30753941] |
Molecular Medicine Reports | MicroRNA‑21 contributes to the puerarin‑induced cardioprotection via suppression of apoptosis and oxidative stress in a cell model of ischemia/reperfusion injury [Pubmed: 31115556] |
Earth and Environmental Science | Germinated-soy milk as a healthy diet to induce high antioxidant enzymes in breast milk [] |
Frontiers in Neuroscience | Hydrogen Sulfide Prevents Sleep Deprivation-Induced Hippocampal Damage by Upregulation of Sirt1 in the Hippocampus [Pubmed: 32218719] |
Oxidative Medicine and Cellular Longevity | Dexmedetomidine Attenuates Orthotopic Liver Transplantation-Induced Acute Gut Injury via α2-Adrenergic Receptor-Dependent Suppression of Oxidative Stress [Pubmed: 31827710] |
Stem Cells International | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 … [Pubmed: 32300366] |
Experimental and Therapeutic Medicine | Antiepileptic effects of exogenous β‑hydroxybutyrate on kainic acid‑induced epilepsy [Pubmed: 33101467] |
BIOLOGICAL TRACE ELEMENT RESEARCH | Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle [Pubmed: 32583225] |
Toxicology | Formaldehyde induces ferroptosis in hippocampal neuronal cells by upregulation of the Warburg effect [33259821] |
CELLULAR AND MOLECULAR LIFE SCIENCES | Glutamine deficiency promotes stemness and chemoresistance in tumor cells through DRP1-induced mitochondrial fragmentation [33895866] |
Exp Ther Med | Naringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic rats [34093766] |
Experimental and Therapeutic Medicine | MicroRNA-145-5p aggravates cell apoptosis and oxidative stress in tongue squamous cell carcinoma [33732346] |
chemosphere | Oxidative stress-and mitochondrial dysfunction-mediated cytotoxicity by silica nanoparticle in lung epithelial cells from metabolomic perspective [33662726] |
Molecular Medicine Reports | Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats [34278476] |
J Healthc Eng | κ-Opioid Receptor Agonist Ameliorates Postoperative Neurocognitive Disorder by Activating the Ca2+/CaMKII/CREB Pathway [34608407] |
Cell Death Discov | Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl rats [Pubmed:35022384] |
J Dairy Sci | Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver disease [Pubmed:35086715] |
Antioxidants | Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice [Pubmed:35740008] |
J Inflamm Res | The κ-Opioid Receptor Agonist U50488H Ameliorates Neuropathic Pain Through the Ca2+/CaMKII/CREB Pathway in Rats [Pubmed:35645576] |
留言咨询