谷胱甘肽(GSH)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Glutathione (GSH) ,etc. by FLIA (Flow Luminescence Immunoassay)
L-Reduced Glutathione
(注:单次混测多因子不超过8个指标 )
- 编号LMA294Ge
- 物种Pan-species (General,通用) 相同的名称,不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围0.1-100ug/mL
- 灵敏度最小可检测剂量小于等于0.033 ug/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
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特异性
本试剂盒用于检测谷胱甘肽(GSH)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的谷胱甘肽(GSH)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 82-99 | 85 |
EDTA plasma(n=5) | 92-101 | 97 |
heparin plasma(n=5) | 86-101 | 90 |
sodium citrate plasma(n=5) | 88-102 | 93 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的谷胱甘肽(GSH)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中谷胱甘肽(GSH)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 92-103% | 92-99% | 82-94% | 89-102% |
EDTA plasma(n=5) | 89-97% | 83-95% | 78-96% | 85-97% |
heparin plasma(n=5) | 91-102% | 84-97% | 98-105% | 95-104% |
sodium citrate plasma(n=5) | 80-88% | 96-103% | 79-94% | 85-102% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠、检测溶液A)标准品或样本50μL及磁珠10μL,加检测溶液A50μL,
37°C酶标板振荡器孵育60分钟;
3. 磁吸洗板3次;
4. 加检测溶液B100μL,37°C振动孵育30分钟;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋涡震荡2分钟后读数。
实验原理
将谷胱甘肽(GSH)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠、标记VEGFA,标记的谷胱甘肽(GSH)等多因子检测试剂盒(流式荧光发光法)和未标记的谷胱甘肽(GSH)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合,然后将未结合物洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的谷胱甘肽(GSH)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
编号 | 适用物种:Pan-species (General,通用) | 应用(仅供研究使用,不用于临床诊断!) |
SPA294Ge01 | 谷胱甘肽(GSH)合成多肽 | Immunogen; Blocking Peptide. |
CPA294Ge11 | 谷胱甘肽(GSH)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA294Ge21 | 谷胱甘肽(GSH)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA294Ge41 | 谷胱甘肽(GSH)人血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
PAA294Ge01 | 谷胱甘肽(GSH)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
LAA294Ge71 | 谷胱甘肽(GSH)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAA294Ge22 | 谷胱甘肽(GSH)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA294Ge21 | 谷胱甘肽(GSH)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
CEA294Ge | 谷胱甘肽(GSH)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA294Ge | 谷胱甘肽(GSH)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA294Ge11 | 谷胱甘肽(GSH)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
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