组胺(HA)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Histamine (HA)
Histamin
- 编号KSA927Ge11
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围1.23-100ng/mL
- 可适灵敏度0.52ng/mL
- 应用Main materials for "Do It(ELISA Kit) Yourself"
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 8597 ¥ 14328 ¥ 25790 ¥ 50148 ¥ 85968
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对Histamine (HA)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| CPA927Ge41 | 组胺(HA)人血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA927Ge11 | 组胺(HA)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA927Ge21 | 组胺(HA)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA927Ge01 | 组胺(HA)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA927Ge71 | 组胺(HA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA927Ge81 | 组胺(HA)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA927Ge21 | 组胺(HA)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEA927Ge | 组胺(HA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA927Ge | 组胺(HA)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA927Ge | 组胺(HA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA927Ge11 | 组胺(HA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It(ELISA Kit) Yourself" |
参考文献
| 杂志 | 参考文献 |
| Allergy & Clinical Immunology | PEST-domain-enriched tyrosine phosphatase and glucocorticoids as regulators of anaphylaxis in mice [Pubmed: 21981059] |
| Asian Pacific Journal of Allergy and Immunology | Comparison of sensitization between β-lactoglobulin and its hydrolysates [Digital: 875] |
| J Chromatogr A | Integrative analysis of proteomics and metabolomics of anaphylactoid reaction induced by Xuesaitong injection [PubMed: 26372445] |
| Neurogastroenterology & Motility | Expression of inducible nitric oxide synthase in mast cells contributes to the reCavia (Guinea pig )lation of inflammatory cytokines in irritable bowel syndrome with diarrhea [Pubmed:26940641] |
| Italian Journal of Animal Science | Induction of immune responses and allergic reactions in piglets by injecting glycinin [doi:1828051X.2016.1144488] |
| Int J Clin Exp Pathol | Effects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanism [files:ijcep0019305.pdf] |
| PLoS One. | Proteomics Study on Nonallergic Hypersensitivity Induced by Compound 4880 and Ovalbumin [pmc:PMC4734762] |
| Int Immunopharmacol. | Evaluation of anaphylactoid constituents in vitro and in vivo. [pubmed:27984711] |
| Journal of Cosmet Dermatol. | Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties [DOI: 10.1111/jocd.12349] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model [Pubmed: 30781605] |
| Royal Society Open Science | Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats [] |
| International Journal of Pharmaceutical Sciences and Research | MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL [] |
| Allergy | Artemisia annua sublingual immunotherapy for seasonal allergic rhinitis: a randomized controlled trial [Pubmed: 32030780] |
| MOLECULAR IMMUNOLOGY | Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection [Pubmed: 32302806] |
| bioscience reports | Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767] |
| Nature Communications | A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy [Pubmed: 32332752] |
| CCR3‑shRNA promotes apoptosis and inhibits chemotaxis and degranulation of mouse mast cells [Pubmed: 32742345] | |
| ANALYTICA CHIMICA ACTA | Multiplexed fluorescence immunoassay combined with magnetic separation using upconversion nanoparticles as multicolor labels for the simultaneous detection of … [Pubmed: 32892931] |
| Annals of Tropical Medicine & Public Health | Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq [] |
| pharmacol res& perspect | Pharmacokinetics and safety of TCMCB07, a melanocortin-4 antagonist peptide in dogs [34014033] |
留言咨询