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S100钙结合蛋白A8(S100A8)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for S100 Calcium Binding Protein A8 (S100A8)

MRP8; MIF; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; NIF; P8; Calgranulin A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8

  • S100钙结合蛋白A8(S100A8)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • S100钙结合蛋白A8(S100A8)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对S100钙结合蛋白A8(S100A8)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
APB792Ra01 S100钙结合蛋白A8(S100A8)活性蛋白 Cell culture; Activity Assays.
RPB792Ra01 S100钙结合蛋白A8(S100A8)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB792Ra01 S100钙结合蛋白A8(S100A8)多克隆抗体 WB; IHC; ICC; IP.
LAB792Ra71 S100钙结合蛋白A8(S100A8)多克隆抗体(生物素标记) WB; IHC; ICC.
LAB792Ra81 S100钙结合蛋白A8(S100A8)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAB792Ra21 S100钙结合蛋白A8(S100A8)单克隆抗体 WB; IHC; ICC; IP.
SEB792Ra S100钙结合蛋白A8(S100A8)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB792Ra S100钙结合蛋白A8(S100A8)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB792Ra01 S100钙结合蛋白A8(S100A8)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Biochemical and Biophysical Research Communications High circulating levels of S100A8/A9 complex (calprotectin) in male Japanese with abdominal adiposity and dysregulated expression of S100A8 and S100A9 in adipose tissues of obese mice [ScienceDirect: S0006291X1200352X]
American Journal of Reproductive Immunology Association of Increased S100A8 Serum Protein with Early Pregnancy Loss [Pubmed:25252120]
the faseb journal Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferation [PubMed: 25934702]
J Immunol S100A8 Production in CXCR2-Expressing CD11b+ Gr-1high Cells Aggravates Hepatitis in Mice Fed a High-Fat and High-Cholesterol Diet [PubMed: 26608915]
PLoS One Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation [Pubmed:26889680]
Journal of Alzheimer's Disease Proteomics Analysis of Blood Serums from Alzheimer's Disease Patients Using iTRAQ LabelingTechnology. [pubmed:27911324]
 Journal of Functional Foods Standardized ethanolic extract of the rhizome of Curcuma xanthorrhiza prevents murine ulcerative colitis by regulation of inflammation [S1756464617300208]
International Journal of Molecular Medicine Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells [pubmed:28498464]
International journal of molecular sciences Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds. [pubmed:28672877]
Gastroenterology Tu1938-Micronutrients Assessment in Patients with Cirrhosis [10.1016:S0016-5085(18)33544-3]
Gastroenterology Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis [10.1016:S0016-5085(18)33544-5]
Journal of Biological Chemistry Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation [Pubmed:29496993]
European review for medical and pharmacological sciences S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα. [Pubmed:29949169]
Foodborne Pathogens and Disease Synergistic effect of immunomodulatory S100A8/A9 and ciprofloxacin against Pseudomonas aeruginosa biofilm in a murine chronic wound model [Pubmed: 31116394]
Kidney International Reports Systemic Inflammation Precedes Microalbuminuria in Diabetes []
Cancer Management and Research iTRAQ-based quantitative protein expression profiling of biomarkers in childhood B-cell and T-cell acute lymphoblastic leukemia [Pubmed: 31440093]
scandinavian journal of clinical & laboratory investigation Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study [Pubmed: 31856598]
ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis []
MOLECULAR VISION Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays [34012227]
university of copenhagen Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in []
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