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S100钙结合蛋白A8(S100A8)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for S100 Calcium Binding Protein A8 (S100A8)

MRP8; MIF; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; NIF; P8; Calgranulin A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8

  • S100钙结合蛋白A8(S100A8)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • S100钙结合蛋白A8(S100A8)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • S100钙结合蛋白A8(S100A8)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEB792Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测S100钙结合蛋白A8(S100A8)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的S100钙结合蛋白A8(S100A8)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 88-99 95
EDTA plasma(n=5) 80-101 87
heparin plasma(n=5) 96-103 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的S100钙结合蛋白A8(S100A8)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中S100钙结合蛋白A8(S100A8)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 81-95% 83-98% 80-90% 87-97%
EDTA plasma(n=5) 86-93% 91-101% 94-105% 82-104%
heparin plasma(n=5) 94-105% 80-90% 85-101% 90-97%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将S100钙结合蛋白A8(S100A8)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的S100钙结合蛋白A8(S100A8)与连接于固相载体上的抗体结合，然后加入生物素化的S100钙结合蛋白A8(S100A8)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的S100钙结合蛋白A8(S100A8)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
APB792Ra01 S100钙结合蛋白A8(S100A8)活性蛋白 Cell culture; Activity Assays.
RPB792Ra01 S100钙结合蛋白A8(S100A8)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB792Ra01 S100钙结合蛋白A8(S100A8)多克隆抗体 WB; IHC; ICC; IP.
LAB792Ra71 S100钙结合蛋白A8(S100A8)多克隆抗体(生物素标记) WB; IHC; ICC.
LAB792Ra81 S100钙结合蛋白A8(S100A8)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAB792Ra21 S100钙结合蛋白A8(S100A8)单克隆抗体 WB; IHC; ICC; IP.
SEB792Ra S100钙结合蛋白A8(S100A8)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB792Ra S100钙结合蛋白A8(S100A8)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB792Ra01 S100钙结合蛋白A8(S100A8)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
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PLoS One Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation [Pubmed:26889680]
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 Journal of Functional Foods Standardized ethanolic extract of the rhizome of Curcuma xanthorrhiza prevents murine ulcerative colitis by regulation of inflammation [S1756464617300208]
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Gastroenterology Tu1938-Micronutrients Assessment in Patients with Cirrhosis [10.1016:S0016-5085(18)33544-3]
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Journal of Biological Chemistry Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation [Pubmed:29496993]
European review for medical and pharmacological sciences S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα. [Pubmed:29949169]
Foodborne Pathogens and Disease Synergistic effect of immunomodulatory S100A8/A9 and ciprofloxacin against Pseudomonas aeruginosa biofilm in a murine chronic wound model [Pubmed: 31116394]
Kidney International Reports Systemic Inflammation Precedes Microalbuminuria in Diabetes []
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scandinavian journal of clinical & laboratory investigation Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study [Pubmed: 31856598]
ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis []
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