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多配体蛋白聚糖1(SDC1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Syndecan 1 (SDC1)

CD138; SDC; SYND1; Syndecan Proteoglycan 1

  • 多配体蛋白聚糖1(SDC1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 多配体蛋白聚糖1(SDC1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对多配体蛋白聚糖1(SDC1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB966Mu02 多配体蛋白聚糖1(SDC1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB966Mu01 多配体蛋白聚糖1(SDC1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPB966Mu62 多配体蛋白聚糖1(SDC1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB966Mu01 多配体蛋白聚糖1(SDC1)多克隆抗体 WB; IHC; ICC; IP.
PAB966Mu02 多配体蛋白聚糖1(SDC1)多克隆抗体 WB; IHC; ICC; IP.
SEB966Mu 多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB966Mu 多配体蛋白聚糖1(SDC1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB966Mu01 多配体蛋白聚糖1(SDC1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Molecular and Cellular Biochemistry Increased level of soluble syndecan-1 in serum correlates with myocardial expression in a rat model of myocardial infarction [SpringerLink: a201w3l18k257462]
Inflammation Sdc1 Overexpression Inhibits the p38 MAPK Pathway and Lessens Fibrotic Ventricular Remodeling in MI Rats [Springer: Source]
Atherosclerosis. Damage of the endothelial glycocalyx in chronic kidney disease [Pubmed:24727235]
Journal of Trauma and Acute Care Surgery Effect of valproic acid and injury on lesion size and endothelial glycocalyx shedding in a rodent model of isolated traumatic brain injury [Pubmed:25058256]
Shock Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance [Pubmed:25692257]
Mediators Inflamm Dexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx Shedding [PubMed: 26199464]
Transplantation Platelet-Derived Growth Factor-B Protects Rat Cardiac Allografts From Ischemia-reperfusion Injury. [PubMed: 26371596]
Clinical and Experimental Nephrology Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injury [pubmed:27405618]
Biomarkers in Medicine Endothelial glycocalyx layer shedding following lung resection [pubmed:27643669]
Danish National RESEARCH DATABASE Resuscitation with Pooled and Pathogen-Reduced Plasma Attenuates the Increase in Brain Water Content following Traumatic Brain Injury and Hemorrhagic Shock in Rats [10.1089]
Journal of Neurotrauma Resuscitation with pooled and pathogen-reduced plasma attenuates the increase in brain water content following traumatic brain injury and hemorrhagic shock in rats the rat. [doi/10.1089/neu.2016.4574]
British Journal of Anaesthesia Volume kinetics of Ringer's lactate solution in acute inflammatory disease [10.1016:j.bja.2018.04.023]
Arteriosclerosis, Thrombosis, and Vascular Biology MPO (myeloperoxidase) reduces endothelial glycocalyx thickness dependent on its cationic charge [Pubmed:29903730]
Shock Plasma resuscitation improved survival in a cecal ligation and puncture rat model of sepsis [Pubmed:28591008]
Shock Experimental models of endotheliopathy: impact of shock severity [Pubmed:28697004]
Journal of Trauma and Acute Care Surgery Effects of propranolol and clonidine on brain edema, blood-brain barrier permeability, and endothelial glycocalyx disruption after fluid percussion brain injury in the rat [Pubmed:28930945]
The Endothelial Glycocalyx in the Peritoneal Microcirculation of Rats with Chronic Kidney Failure Exposed to Dialysis Solutions [173015_06.pdf]
Journal of Surgical Research Plasma ameliorates endothelial dysfunction in burn injury [Doi: 10.1016/j.jss.2018.08.027]
Journal of Anesthesia Dexmedetomidine preserves the endothelial glycocalyx and improves survival in a rat heatstroke model [Pubmed: 30374889]
Anaesthesia Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass [Pubmed: 30687934]
Journal of Cardiothoracic and Vascular Anesthesial Microvascular alterations during cardiac surgery using a heparin or phosphorylcholine coated circuit [Pubmed: 31787433]
Hepatobiliary & Pancreatic Diseases International Increased syndecan-1 and glypican-3 predict poor perinatal outcome and treatment resistance in intrahepatic cholestasis: Syndecan-1, glypican-3 and ICP [Pubmed: 31919038]
Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study []
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Newly Developed Recombinant Antithrombin Protects the Endothelial Glycocalyx in an Endotoxin-Induced Rat Model of Sepsis [33375342]
Microvasc Res Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency [34119534]
J Gerontol A Biol Sci Med Sci The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo … [34423832]
Shock Inhalation of 2% hydrogen improves survival rate and attenuates shedding of vascular endothelial glycocalyx in rats with heat stroke [34524269]
Exp Anim Dexmedetomidine suppresses serum syndecan-1 elevation and improves survival in a rat hemorrhagic shock model [Pubmed:35110424]
Journal of Emergency Medicine Resuscitation of hemorrhagic shock using normal saline does not damage the glycocalyx in the immediate resuscitation phase []
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