多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Syndecan 1 (SDC1)
CD138; SDC; SYND1; Syndecan Proteoglycan 1
- 编号SEB966Mu
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围1.56-100ng/mL
- 灵敏度最小可检测剂量小于等于0.57ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2873 ¥ 4104 ¥ 18468 ¥ 34884 ¥ 287280
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测多配体蛋白聚糖1(SDC1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的多配体蛋白聚糖1(SDC1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 89-97 | 92 |
| EDTA plasma(n=5) | 95-102 | 99 |
| heparin plasma(n=5) | 78-98 | 89 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的多配体蛋白聚糖1(SDC1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中多配体蛋白聚糖1(SDC1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-105% | 80-103% | 80-93% | 78-90% |
| EDTA plasma(n=5) | 84-101% | 91-99% | 78-91% | 82-97% |
| heparin plasma(n=5) | 91-98% | 80-94% | 84-92% | 89-98% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将多配体蛋白聚糖1(SDC1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的多配体蛋白聚糖1(SDC1)与连接于固相载体上的抗体结合，然后加入生物素化的多配体蛋白聚糖1(SDC1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的多配体蛋白聚糖1(SDC1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB966Mu02 | 多配体蛋白聚糖1(SDC1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB966Mu01 | 多配体蛋白聚糖1(SDC1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB966Mu62 | 多配体蛋白聚糖1(SDC1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB966Mu01 | 多配体蛋白聚糖1(SDC1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB966Mu02 | 多配体蛋白聚糖1(SDC1)多克隆抗体 | WB; IHC; ICC; IP. |
| SEB966Mu | 多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB966Mu | 多配体蛋白聚糖1(SDC1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB966Mu01 | 多配体蛋白聚糖1(SDC1)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Molecular and Cellular Biochemistry | Increased level of soluble syndecan-1 in serum correlates with myocardial expression in a rat model of myocardial infarction [SpringerLink: a201w3l18k257462] |
| Inflammation | Sdc1 Overexpression Inhibits the p38 MAPK Pathway and Lessens Fibrotic Ventricular Remodeling in MI Rats [Springer: Source] |
| Atherosclerosis. | Damage of the endothelial glycocalyx in chronic kidney disease [Pubmed:24727235] |
| Journal of Trauma and Acute Care Surgery | Effect of valproic acid and injury on lesion size and endothelial glycocalyx shedding in a rodent model of isolated traumatic brain injury [Pubmed:25058256] |
| Shock | Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance [Pubmed:25692257] |
| Mediators Inflamm | Dexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx Shedding [PubMed: 26199464] |
| Transplantation | Platelet-Derived Growth Factor-B Protects Rat Cardiac Allografts From Ischemia-reperfusion Injury. [PubMed: 26371596] |
| Clinical and Experimental Nephrology | Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injury [pubmed:27405618] |
| Biomarkers in Medicine | Endothelial glycocalyx layer shedding following lung resection [pubmed:27643669] |
| Danish National RESEARCH DATABASE | Resuscitation with Pooled and Pathogen-Reduced Plasma Attenuates the Increase in Brain Water Content following Traumatic Brain Injury and Hemorrhagic Shock in Rats [10.1089] |
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| British Journal of Anaesthesia | Volume kinetics of Ringer's lactate solution in acute inflammatory disease [10.1016:j.bja.2018.04.023] |
| Arteriosclerosis, Thrombosis, and Vascular Biology | MPO (myeloperoxidase) reduces endothelial glycocalyx thickness dependent on its cationic charge [Pubmed:29903730] |
| Shock | Plasma resuscitation improved survival in a cecal ligation and puncture rat model of sepsis [Pubmed:28591008] |
| Shock | Experimental models of endotheliopathy: impact of shock severity [Pubmed:28697004] |
| Journal of Trauma and Acute Care Surgery | Effects of propranolol and clonidine on brain edema, blood-brain barrier permeability, and endothelial glycocalyx disruption after fluid percussion brain injury in the rat [Pubmed:28930945] |
| The Endothelial Glycocalyx in the Peritoneal Microcirculation of Rats with Chronic Kidney Failure Exposed to Dialysis Solutions [173015_06.pdf] | |
| Journal of Surgical Research | Plasma ameliorates endothelial dysfunction in burn injury [Doi: 10.1016/j.jss.2018.08.027] |
| Journal of Anesthesia | Dexmedetomidine preserves the endothelial glycocalyx and improves survival in a rat heatstroke model [Pubmed: 30374889] |
| Anaesthesia | Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass [Pubmed: 30687934] |
| Journal of Cardiothoracic and Vascular Anesthesial | Microvascular alterations during cardiac surgery using a heparin or phosphorylcholine coated circuit [Pubmed: 31787433] |
| Hepatobiliary & Pancreatic Diseases International | Increased syndecan-1 and glypican-3 predict poor perinatal outcome and treatment resistance in intrahepatic cholestasis: Syndecan-1, glypican-3 and ICP [Pubmed: 31919038] |
| Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study [] | |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Newly Developed Recombinant Antithrombin Protects the Endothelial Glycocalyx in an Endotoxin-Induced Rat Model of Sepsis [33375342] |
| Microvasc Res | Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency [34119534] |
| J Gerontol A Biol Sci Med Sci | The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo … [34423832] |
| Shock | Inhalation of 2% hydrogen improves survival rate and attenuates shedding of vascular endothelial glycocalyx in rats with heat stroke [34524269] |
| Exp Anim | Dexmedetomidine suppresses serum syndecan-1 elevation and improves survival in a rat hemorrhagic shock model [Pubmed:35110424] |
| Journal of Emergency Medicine | Resuscitation of hemorrhagic shock using normal saline does not damage the glycocalyx in the immediate resuscitation phase [] |
