神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Enolase, Neuron Specific (NSE)
ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase
- 编号SEA537Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.625-40ng/mL
- 灵敏度最小可检测剂量小于等于0.235ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2646 ¥ 3780 ¥ 17010 ¥ 32130 ¥ 264600
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测神经元特异性烯醇化酶(NSE)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的神经元特异性烯醇化酶(NSE)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 81-91 | 88 |
| EDTA plasma(n=5) | 78-98 | 90 |
| heparin plasma(n=5) | 82-95 | 86 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的神经元特异性烯醇化酶(NSE)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中神经元特异性烯醇化酶(NSE)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-101% | 88-95% | 83-90% | 88-97% |
| EDTA plasma(n=5) | 81-102% | 98-105% | 96-105% | 86-102% |
| heparin plasma(n=5) | 92-101% | 88-102% | 89-96% | 89-99% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将神经元特异性烯醇化酶(NSE)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的神经元特异性烯醇化酶(NSE)与连接于固相载体上的抗体结合，然后加入生物素化的神经元特异性烯醇化酶(NSE)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的神经元特异性烯醇化酶(NSE)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA537Hu02 | 神经元特异性烯醇化酶(NSE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA537Hu01 | 神经元特异性烯醇化酶(NSE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA537Hu01 | 神经元特异性烯醇化酶(NSE)多克隆抗体 | WB,IHC |
| PAA537Hu02 | 神经元特异性烯醇化酶(NSE)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA537Hu71 | 神经元特异性烯醇化酶(NSE)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA537Hu81 | 神经元特异性烯醇化酶(NSE)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA537Hu27 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu29 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu25 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu21 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu22 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu23 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu26 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu28 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu24 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA537Hu | 神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA537Hu | 神经元特异性烯醇化酶(NSE)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA537Hu01 | 神经元特异性烯醇化酶(NSE)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
| CGA537Hu01 | 神经元特异性烯醇化酶(NSE)cDNA | PCR、蛋白表达等 |
参考文献
| 杂志 | 参考文献 |
| Journal of Neurotrauma | The Anti-Inflammatory and Neuroprotective Effects of Ghrelin in Subarachnoid Hemorrhage-Induced Oxidative Brain Damage in Rats [Liebert: 20091210] |
| 20 | Melatonin treatment protects against spinal cord injury induced functional and biochemical changes in rat urinary bladder [Wiley: source] |
| Journal of Pharmacology and Toxicology | Ginkgo biloba Enhances the Anticonvulsant and Neuroprotective Effects of Sodium Valproate Against Kainic Acid-induced Seizures in Mice [Medwell: 2011679690] |
| Addiction Biology | Comparative gene expression profiling analysis of lymphoblastoid cells reveals neuron‐specific enolase gene (ENO2) as a susceptibility gene of heroin dependence [PubMed: 21995595] |
| Placenta | Advanced glycation end products as an upstream molecule triggers ROS-induced sFlt-1 production in extravillous trophoblasts: A novel bridge between oxidative stress and preeclampsia [Pubmed: 24144948] |
| J Ethnopharmacol. | Therapeutic time window and underlying therapeutic mechanism of breviscapine injection against cerebral ischemia/reperfusion injury in rats. [Pubmed: 24291152] |
| J Neurosurg. | Detection of β-amyloid oligomers as a predictor of neurological outcome after brain injury. [Pubmed: 23540266] |
| Analytical Methods | A portable and quantitative enzyme immunoassay of neuron-specific enolase with a glucometer read out [Rsc: Source] |
| Int J Neurosci. | The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbits [Pubmed:25111247] |
| Experimental & Molecular Medicine | Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns [Pubmed:25358349] |
| Neuroscience Letters | Preconditioning effect of (S)-3,5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague-Dawley rats. [Pubmed:25562631] |
| 中华临床医师杂志 | 高迁移率族蛋白B1和神经元烯醇化酶对心脏骤停大鼠复苏后脑损伤评价的研究 [Journal: Fulltext] |
| Am J Trop Med Hyg | Blood–Brain Barrier Function and Biomarkers of Central Nervous System Injury in Rickettsial versus Other Neurological Infections in Laos [PubMed: 26055741] |
| Neurosci Lett | Preconditioning effect of (S)-3, 5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague–Dawley rats [PubMed: 25562631] |
| Physiol Rep | S100B and NSE serum concentrations after simulated diving in rats [PubMed: 26462746] |
| Int J Mol Sci | The effect of electroporation of a lyotroic liquid crystal Genistein-based formulation in the recovery of murine melanoma lesions [PubMed: 26184156] |
| PLoS One | Isoflurane Post-Treatment Improves Outcome after an Embolic Stroke in Rabbits [PubMed: 26645542] |
| Фармакология, клиническая фармакология | Волгоградский государственный медицинский университет [uploads:dsovet] |
| Analytical Methods | Synthesis of sunflower-like gold nanostructures and their application in electrochemical immunoassays using the nanogold-triggered hydrogen evolution reaction [articlelanding:c5ay03174e] |
| 中国临床药理学与治疗学 | 右美托咪定对脑膜瘤切除术患者血清S100β 蛋白及神经元特异性烯醇化酶的影响 [article:9794] |
| Asian Pacific Journal of Tropical Medicine | Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury [science:S1995764516300955] |
| Izvorni znanstveni članak | SERUMSKA RAZINA ZA NEURON SPECIFIČNE ENOLAZE KAO BILJEG ISHEMIJSKO-REPERFUZIJSKOG OŠTEĆENJA U BOLESNIKA PODVRGNUTIH KAROTIDNOJ ENDARTEREKTOMIJI [262238] |
| Neurourol Urodyn | Functional and structural changes of the urinary bladder following spinal cord injury; treatment with alpha lipoic acid [pubmed:27490041] |
| Biomedicine & Pharmacotherapy | Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats [pubmed:28076830] |
| cellular physiology and biochemistry | Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β … [pubmed:29040978] |
| Brain Research | Intra-arterial human urinary kallidinogenase alleviates brain injury in rats with permanent middle cerebral artery occlusion through PI3K/AKT/FoxO1 signaling pathway [Pubmed:29510144] |
| Biomed Research International | Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway [Pubmed:29770336] |
| Journal of the Neurological Sciences | S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid … [Pubmed:29801873] |
| Iranian Journal of Veterinary Research | Nitrosative stress indices in dogs with neurological form of canine distemper [Doi: 10.22099/IJVR.2018.4943] |
| Evidence-Based Complementary and Alternative Medicine | Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway [] |
| The Eurasian Journal of Medicine | Effects of Conivaptan versus Mannitol on Post-Ischemic Brain Injury and Edema [] |
| Medicina | The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the … [] |
| ACTA NEUROBIOLOGIAE EXPERIMENTALIS | Neuroprotective effect of poly (lactic-co-glycolic acid) nanoparticle-bound brain-derived neurotrophic factor in a permanent middle cerebral artery occlusion … [Pubmed: 32214270] |
| Stem Cells International | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 … [Pubmed: 32300366] |
| European Review for Medical and Pharmacological Sciences | Neuroprotective effect of CTRP3 overexpression against sevoflurane anesthesia-induced cognitive dysfunction in aged rats through activating AMPK/SIRT1 and … [Pubmed: 32432773] |
| Neurobiology of Learning and Memory | Effects of Circadian Rhythm Disorder on the Hippocampus of SHR and WKY Rats [] |
| The role of light desynchronosis in the development of stress-induced aging [] | |
| ИЗУЧЕНИЕ ЦЕРЕБРОПРОТЕКТОРНОГО ДЕЙСТВИЯ ПРЕПАРАТОВ РАЗЛИЧНЫХ ФАРМАКОЛОГИЧЕСКИХ ГРУПП В УСЛОВИЯХ … [] | |
| Chinese Journal of Analytical Chemistry | Investigation of Library Input on Aptamers Selection Efficiency Using Capillary Electrophoresis [] |
| Ther Adv Chronic Dis | The effect of doxycycline on neuron-specific enolase in patients with traumatic brain injury: a randomized controlled trial [34262678] |
| J Surg Res | Perillaldehyde Alleviates Spinal Cord Ischemia-Reperfusion Injury Via Activating the Nrf2 Pathway [34399353] |
| Cephalalgia | Evaluation of serum neuron specific enolase levels among patients with primary and secondary burning mouth syndrome [34644195] |
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