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神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Enolase, Neuron Specific (NSE)

ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase

  • 神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA537Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测神经元特异性烯醇化酶(NSE),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的神经元特异性烯醇化酶(NSE)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 81-91 88
EDTA plasma(n=5) 78-98 90
heparin plasma(n=5) 82-95 86

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的神经元特异性烯醇化酶(NSE),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中神经元特异性烯醇化酶(NSE)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 94-101% 88-95% 83-90% 88-97%
EDTA plasma(n=5) 81-102% 98-105% 96-105% 86-102%
heparin plasma(n=5) 92-101% 88-102% 89-96% 89-99%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将神经元特异性烯醇化酶(NSE)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的神经元特异性烯醇化酶(NSE)与连接于固相载体上的抗体结合,然后加入生物素化的神经元特异性烯醇化酶(NSE)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的神经元特异性烯醇化酶(NSE)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA537Hu02 神经元特异性烯醇化酶(NSE)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA537Hu01 神经元特异性烯醇化酶(NSE)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA537Hu01 神经元特异性烯醇化酶(NSE)多克隆抗体 WB,IHC
PAA537Hu02 神经元特异性烯醇化酶(NSE)多克隆抗体 WB; IHC; ICC; IP.
LAA537Hu71 神经元特异性烯醇化酶(NSE)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA537Hu81 神经元特异性烯醇化酶(NSE)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA537Hu27 神经元特异性烯醇化酶(NSE)单克隆抗体 WB; IHC; ICC; IP.
MAA537Hu29 神经元特异性烯醇化酶(NSE)单克隆抗体 WB; IHC; ICC; IP.
MAA537Hu25 神经元特异性烯醇化酶(NSE)单克隆抗体 WB; IHC; ICC; IP.
MAA537Hu21 神经元特异性烯醇化酶(NSE)单克隆抗体 WB; IHC; ICC; IP.
MAA537Hu22 神经元特异性烯醇化酶(NSE)单克隆抗体 WB; IHC; ICC; IP.
MAA537Hu23 神经元特异性烯醇化酶(NSE)单克隆抗体 WB; IHC; ICC; IP.
MAA537Hu26 神经元特异性烯醇化酶(NSE)单克隆抗体 WB; IHC; ICC; IP.
MAA537Hu28 神经元特异性烯醇化酶(NSE)单克隆抗体 WB; IHC; ICC; IP.
MAA537Hu24 神经元特异性烯醇化酶(NSE)单克隆抗体 WB; IHC; ICC; IP.
SEA537Hu 神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA537Hu 神经元特异性烯醇化酶(NSE)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA537Hu01 神经元特异性烯醇化酶(NSE)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".
CGA537Hu01 神经元特异性烯醇化酶(NSE)cDNA PCR、蛋白表达等

参考文献

杂志 参考文献
Journal of Neurotrauma The Anti-Inflammatory and Neuroprotective Effects of Ghrelin in Subarachnoid Hemorrhage-Induced Oxidative Brain Damage in Rats [Liebert: 20091210]
20 Melatonin treatment protects against spinal cord injury induced functional and biochemical changes in rat urinary bladder [Wiley: source]
Journal of Pharmacology and Toxicology Ginkgo biloba Enhances the Anticonvulsant and Neuroprotective Effects of Sodium Valproate Against Kainic Acid-induced Seizures in Mice [Medwell: 2011679690]
Addiction Biology Comparative gene expression profiling analysis of lymphoblastoid cells reveals neuron‐specific enolase gene (ENO2) as a susceptibility gene of heroin dependence [PubMed: 21995595]
Placenta Advanced glycation end products as an upstream molecule triggers ROS-induced sFlt-1 production in extravillous trophoblasts: A novel bridge between oxidative stress and preeclampsia [Pubmed: 24144948]
J Ethnopharmacol. Therapeutic time window and underlying therapeutic mechanism of breviscapine injection against cerebral ischemia/reperfusion injury in rats. [Pubmed: 24291152]
J Neurosurg. Detection of β-amyloid oligomers as a predictor of neurological outcome after brain injury. [Pubmed: 23540266]
Analytical Methods A portable and quantitative enzyme immunoassay of neuron-specific enolase with a glucometer read out [Rsc: Source]
Int J Neurosci. The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbits [Pubmed:25111247]
Experimental & Molecular Medicine Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns [Pubmed:25358349]
Neuroscience Letters Preconditioning effect of (S)-3,5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague-Dawley rats. [Pubmed:25562631]
中华临床医师杂志 高迁移率族蛋白B1和神经元烯醇化酶对心脏骤停大鼠复苏后脑损伤评价的研究 [Journal: Fulltext]
Am J Trop Med Hyg Blood–Brain Barrier Function and Biomarkers of Central Nervous System Injury in Rickettsial versus Other Neurological Infections in Laos [PubMed: 26055741]
Neurosci Lett Preconditioning effect of (S)-3, 5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague–Dawley rats [PubMed: 25562631]
Physiol Rep S100B and NSE serum concentrations after simulated diving in rats [PubMed: 26462746]
Int J Mol Sci The effect of electroporation of a lyotroic liquid crystal Genistein-based formulation in the recovery of murine melanoma lesions [PubMed: 26184156]
PLoS One Isoflurane Post-Treatment Improves Outcome after an Embolic Stroke in Rabbits [PubMed: 26645542]
Фармакология, клиническая фармакология Волгоградский государственный медицинский университет [uploads:dsovet]
Analytical Methods Synthesis of sunflower-like gold nanostructures and their application in electrochemical immunoassays using the nanogold-triggered hydrogen evolution reaction [articlelanding:c5ay03174e]
中国临床药理学与治疗学 右美托咪定对脑膜瘤切除术患者血清S100β 蛋白及神经元特异性烯醇化酶的影响 [article:9794]
Asian Pacific Journal of Tropical Medicine Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury [science:S1995764516300955]
Izvorni znanstveni članak SERUMSKA RAZINA ZA NEURON SPECIFIČNE ENOLAZE KAO BILJEG ISHEMIJSKO-REPERFUZIJSKOG OŠTEĆENJA U BOLESNIKA PODVRGNUTIH KAROTIDNOJ ENDARTEREKTOMIJI [262238]
Neurourol Urodyn Functional and structural changes of the urinary bladder following spinal cord injury; treatment with alpha lipoic acid [pubmed:27490041]
Biomedicine & Pharmacotherapy Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats [pubmed:28076830]
cellular physiology and biochemistry Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β … [pubmed:29040978]
Brain Research Intra-arterial human urinary kallidinogenase alleviates brain injury in rats with permanent middle cerebral artery occlusion through PI3K/AKT/FoxO1 signaling pathway [Pubmed:29510144]
Biomed Research International Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway [Pubmed:29770336]
Journal of the Neurological Sciences S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid … [Pubmed:29801873]
Iranian Journal of Veterinary Research Nitrosative stress indices in dogs with neurological form of canine distemper [Doi: 10.22099/IJVR.2018.4943]
Evidence-Based Complementary and Alternative Medicine Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway []
The Eurasian Journal of Medicine Effects of Conivaptan versus Mannitol on Post-Ischemic Brain Injury and Edema []
Medicina The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the … []
ACTA NEUROBIOLOGIAE EXPERIMENTALIS Neuroprotective effect of poly (lactic-co-glycolic acid) nanoparticle-bound brain-derived neurotrophic factor in a permanent middle cerebral artery occlusion … [Pubmed: 32214270]
Stem Cells International Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 … [Pubmed: 32300366]
European Review for Medical and Pharmacological Sciences Neuroprotective effect of CTRP3 overexpression against sevoflurane anesthesia-induced cognitive dysfunction in aged rats through activating AMPK/SIRT1 and … [Pubmed: 32432773]
Neurobiology of Learning and Memory Effects of Circadian Rhythm Disorder on the Hippocampus of SHR and WKY Rats []
The role of light desynchronosis in the development of stress-induced aging []
ИЗУЧЕНИЕ ЦЕРЕБРОПРОТЕКТОРНОГО ДЕЙСТВИЯ ПРЕПАРАТОВ РАЗЛИЧНЫХ ФАРМАКОЛОГИЧЕСКИХ ГРУПП В УСЛОВИЯХ … []
Chinese Journal of Analytical Chemistry Investigation of Library Input on Aptamers Selection Efficiency Using Capillary Electrophoresis []
Ther Adv Chronic Dis The effect of doxycycline on neuron-specific enolase in patients with traumatic brain injury: a randomized controlled trial [34262678]
J Surg Res Perillaldehyde Alleviates Spinal Cord Ischemia-Reperfusion Injury Via Activating the Nrf2 Pathway [34399353]
Cephalalgia Evaluation of serum neuron specific enolase levels among patients with primary and secondary burning mouth syndrome [34644195]
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