免疫球蛋白E(IgE)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Immunoglobulin E (IgE)
IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region
- 编号SEA545Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围7.8-500ng/mL
- 灵敏度最小可检测剂量小于等于2.9ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2646 ¥ 3780 ¥ 17010 ¥ 32130 ¥ 264600
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测免疫球蛋白E(IgE)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的免疫球蛋白E(IgE)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 90-98 | 93 |
| EDTA plasma(n=5) | 96-103 | 101 |
| heparin plasma(n=5) | 97-105 | 101 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的免疫球蛋白E(IgE)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中免疫球蛋白E(IgE)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-99% | 94-105% | 91-101% | 81-88% |
| EDTA plasma(n=5) | 93-101% | 82-101% | 88-101% | 95-103% |
| heparin plasma(n=5) | 78-99% | 98-105% | 79-97% | 99-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将免疫球蛋白E(IgE)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的免疫球蛋白E(IgE)与连接于固相载体上的抗体结合，然后加入生物素化的免疫球蛋白E(IgE)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的免疫球蛋白E(IgE)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| NPA545Hu01 | 免疫球蛋白E(IgE)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA545Hu01 | 免疫球蛋白E(IgE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA545Hu01 | 免疫球蛋白E(IgE)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA545Hu71 | 免疫球蛋白E(IgE)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| RAA545Hu21 | 免疫球蛋白E(IgE)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
| MAA545Hu22 | 免疫球蛋白E(IgE)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA545Hu | 免疫球蛋白E(IgE)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA545Hu | 免疫球蛋白E(IgE)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA545Hu01 | 免疫球蛋白E(IgE)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Molecular Carcinogenesis | IL17A gene polymorphisms, serum IL-17A and IgE levels, and hepatocellular carcinoma risk in patients with chronic hepatitis B virus infection [PubMed: 23280722] |
| Human Immunology | IL21 and IL21R polymorphisms and their interactive effects on serum IL-21 and IgE levels in patients with chronic hepatitis B virus infection [PubMed: 23354321] |
| Scand J Immunol | Passive transfer of tumour-derived MDSCs inhibits asthma-related airway inflammation [Pubmed: 24313384] |
| AAPS PharmSciTech | Topical Lyogel Containing Corticosteroid Decreases IgE Expression and Enhances the Therapeutic Efficacy Against Atopic Eczema [PubMed: 25511806] |
| Ann Allergy Asthma Immunol | Reduced airway microbiota diversity is associated with elevated allergic respiratory inflammation [PubMed: 26123423] |
| Molecular Medicine Reports | The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-β/Smad signaling and activation of the Nrf2/HO-1 pathway. [pubmed:27484042] |
| Gut Pathog. | Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice [pubmed:28250847] |
| International Immunopharmacology | Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lung [pubmed:27992791] |
| Asian Pacific Society of Respirology | Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice [pubmed:28122397] |
| Molecular Medicine Reports | Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt. [pubmed:28586019] |
| Journal of Autoimmunity | Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice [10.1016:j.jaut.2018.07.003] |
| Water Research | Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model [Pubmed:29655095] |
| Human & Experimental Toxicology | Investigation of hypersensitivity potential of diacetyl by determining cytokine profiles [Pubmed:29233007] |
| Journal of International Medical Research | Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease [Pubmed:29950127] |
| Journal Of Cellular Biochemistry | Effects of catalpol on bronchial asthma and its relationship with cytokines [Pubmed: 30536454] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model [Pubmed: 30781605] |
| Royal Society Open Science | Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats [] |
| Health and Medicine | Antiallergical Effect of New Combined Nazal Aerodisperse System in the Conditions of Experimental Allergic Rhinitis [] |
| American Journal of Translational Research | Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression [Pubmed: 31737193] |
| MOLECULAR IMMUNOLOGY | Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection [Pubmed: 32302806] |
| bioscience reports | Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767] |
| international journal of antimicrobial agents | Synergistic Phage-surfactant Combination Clears IgE-promoted Staphylococcus aureus Aggregation in Vitro and Enhances the Effect in Vivo [Pubmed: 32335278] |
| Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota [] | |
| ACS Biomaterials Science & Engineering | Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction [] |
| Biomarkers of Allergic Asthma and their Association with Serum Parameters [] | |
| Biomed Pharmacother | Qingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic mice [33706133] |
| Environ Pollut | Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure [34090073] |
| Annals of Tropical Medicine & Public Health | Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity [] |
| Mediators Inflamm | Roles Played by the PI3K/Akt/HIF-1α Pathway and IL-17A in the Chinese Subtype of Chronic Sinusitis with Nasal Polyps [Pubmed:35075349] |
| Expert Opinion on Therapeutic Targets | Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthma [Pubmed:35549595] |
