脂多糖结合蛋白(LBP)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Lipopolysaccharide Binding Protein (LBP)
LPS-Binding Protein
- 编号SEB406Mu
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围1.56-100ng/mL
- 灵敏度最小可检测剂量小于等于0.62ng/mL.
- 样本类型Serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2873 ¥ 4104 ¥ 18468 ¥ 34884 ¥ 287280
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
实验结果图
-
标准曲线图
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测脂多糖结合蛋白(LBP)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的脂多糖结合蛋白(LBP)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 95-104 | 98 |
| EDTA plasma(n=5) | 91-103 | 96 |
| heparin plasma(n=5) | 92-102 | 98 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的脂多糖结合蛋白(LBP)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中脂多糖结合蛋白(LBP)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-105% | 87-96% | 78-104% | 93-102% |
| EDTA plasma(n=5) | 78-91% | 84-103% | 78-101% | 87-101% |
| heparin plasma(n=5) | 80-103% | 97-104% | 82-101% | 95-102% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将脂多糖结合蛋白(LBP)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的脂多糖结合蛋白(LBP)与连接于固相载体上的抗体结合，然后加入生物素化的脂多糖结合蛋白(LBP)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的脂多糖结合蛋白(LBP)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| APB406Mu01 | 脂多糖结合蛋白(LBP)活性蛋白 | Cell culture; Activity Assays. |
| RPB406Mu02 | 脂多糖结合蛋白(LBP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB406Mu01 | 脂多糖结合蛋白(LBP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB406Mu02 | 脂多糖结合蛋白(LBP)活性蛋白 | Cell culture; Activity Assays. |
| PAB406Mu01 | 脂多糖结合蛋白(LBP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB406Mu02 | 脂多糖结合蛋白(LBP)多克隆抗体 | WB; IHC; ICC; IP. |
| SEB406Mu | 脂多糖结合蛋白(LBP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB406Mu | 脂多糖结合蛋白(LBP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| 37 | A Marker of Endotoxemia Is Associated With Obesity and Related Metabolic Disorders in Apparently Healthy Chinese [Diabetes: source] |
| AIDS | Oral serum-derived bovine immunoglobulin improves duodenal immune reconstitution and absorption function in patients with HIV enteropathy [PubMed: PMC3754419] |
| 31 | Gut microbiome composition is linked to whole grain-induced immunological improvements [Pubmed: 23038174] |
| The Veterinary Journal | High ambient temperature alleviates the inflammatory response and growth depression in pigs challenged with Escherichia coli lipopolysaccharide [Pubmed: 24792207] |
| Perfusion | The effect of normovolemic modified ultrafiltration on inflammatory mediators, endotoxins, terminal complement complexes and clinical outcome in high-risk cardiac surgery patients [Pubmed: 23429100] |
| FEMS Microbiology Ecology | A gut microbiota-targeted dietary intervention for amelioration of chronic inflammation underlying metabolic syndrome. [Pubmed: 24117923] |
| Digestive diseases and sciences | Frequency and Severity of Cirrhotic Cardiomyopathy and Its Possible Relationship with Bacterial Endotoxemia [Pubmed: 23907333] |
| Atherosclerosis | Circulating pathogen associated molecular pattern – Binding proteins and High Mobility Group Box protein 1 in nascent metabolic syndrome: Implications for cellular Toll-like receptor activity [Pubmed:25063948] |
| BMC Complementary and Alternative Medicine | Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study [Pubmed:25652121] |
| Atherosclerosis | Increased adipose tissue secretion of Fetuin-A, lipopolysaccharide-binding protein and high-mobility group box protein 1 in metabolic syndrome [PubMed: 25978344] |
| Annals of Gastroenterology | Systemic levels of human β-defensin 1 are elevated in patients with cirrhosis [PubMed: 26751578] |
| Food Chem. | Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk [Pubmed:27173528] |
| Liver International | High serum lipopolysaccharide binding protein is associated with increased mortality in patients with decompensated cirrhosis [pubmed:27712029] |
| Journal of Functional Foods | Modulation of gut microbiota and gut-generated metabolites by bitter melon results in improvement in the metabolic status in high fat diet-induced obese rats [10.1016/j.jff.2017.12.050] |
| Scientific Reports | Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains. [pubmed:28512356] |
| Journal of ethnopharmacology | A standardized extract of the fruit of Hovenia dulcis alleviated alcohol-induced hangover in healthy subjects with heterozygous ALDH2: A randomized, controlled, crossover trial. [pubmed:28750942] |
| Journal of Allergy and Clinical Immunology | On a Western diet, APOE ɛ4 is associated with low innate immune sensing, but not APOE ɛ3 [Pubmed:29928926] |
| Journal of Animal Sciences | Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rations [Pubmed:30060086] |
| Journal of Animal Science | Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood … [Pubmed: 30184125] |
| BMC Complementary and Alternative Medicine | Evaluation of the effect of Lactobacillus reuteri V3401 on biomarkers of inflammation, cardiovascular risk and liver steatosis in obese adults with metabolic … [Pubmed: 30453950] |
| Cell Communication and Signaling | Oridonin protects LPS-induced acute lung injury by modulating Nrf2-mediated oxidative stress and Nrf2-independent NLRP3 and NF-κB pathways [] |
| Nutrients | High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights … [] |
| Journal of Animal Science | Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers [Pubmed: 31410465] |
| Nutrients | Lactobacillus reuteri V3401 Reduces Inflammatory Biomarkers and Modifies the Gastrointestinal Microbiome in Adults with Metabolic Syndrome: The PROSIR Study [Pubmed: 31370223] |
| journal of veterinary pharmacology and therapeutics | Effects of sinomenine in LPS‐associated diseases are related to inhibition of LBP, Mac‐1, and L‐selectin levels [Pubmed: 31490576] |
| Amyloid | Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril protein [Pubmed: 31615282] |
| Food Research International | Ethanol Extract of Propolis Prevents High-Fat Diet-Induced Insulin Resistance and Obesity in Association with Modulation of Gut Microbiota in Mice [Pubmed: 32156386] |
| Journal of Translational Medicine | Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activation [Pubmed: 33036625] |
| Communications Biology | Oxidation of fish oil exacerbates alcoholic liver disease by enhancing intestinal dysbiosis in mice [Pubmed: 32879433] |
| MOLECULAR NUTRITION & FOOD RESEARCH | Antibiotic Treatment Reduces the Health Benefits of Soy Protein [Pubmed: 32729948] |
| JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | Highly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced Obesity [Pubmed: 32633953] |
| fish & shellfish immunology | Molecular characterization and expression analysis of chemokine (CXCL12) from Nile tilapia (Oreochromis niloticus) [Pubmed: 32540504] |
| Scientific Reports | Tandem Mass Tag (TMT)-based quantitative proteomics reveals potential targets associated with onset of Sub-clinical Mastitis in cows [Pubmed: 32518370] |
| experimental eye research | Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitis [Pubmed: 32376471] |
| Frontiers in Microbiology | Naringin Attenuates High Fat Diet Induced Non-alcoholic Fatty Liver Disease and Gut Bacterial Dysbiosis in Mice [] |
| Journal of Burn Care & Research | Umbilical cord mesenchymal stem cells (hUCMSCs) for inflammatory regulation after excision and grafting of severe burn wounds in rats [33313794] |
| JOURNAL OF ANIMAL SCIENCE | Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient?¡ [33474564] |
| journal of agricultural and food chemistry | Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats [33497213] |
| JOURNAL OF HAZARDOUS MATERIALS | Melatonin alleviates Ochratoxin A-induced liver inflammation involved intestinal microbiota homeostasis and intestinal microbiota-independent manner [33582472] |
| Doctoral Thesis | Studies on Mastitis Caused by Translocated-Bacterial Components in Ruminants [] |
| Arthritis Res Ther | Monocyte transcriptomes from patients with axial spondyloarthritis reveal dysregulated monocytopoiesis and a distinct inflammatory imprint [34560894] |
| Microorganisms | Composition and Functions of the Gut Microbiome in Pediatric Obesity: Relationships with Markers of Insulin Resistance [34361925] |
| Antioxidants | The Prebiotic Potential of Geraniin and Geraniin-Enriched Extract against High-Fat-Diet-Induced Metabolic Syndrome in Sprague Dawley Rats [Pubmed:35453317] |
| Microbiome | Elucidating the role of the gut microbiota in the physiological effects of dietary fiber [Pubmed:35562794] |
| Bioscience of Microbiota Food and Health | Local free fatty acids trigger the expression of lipopolysaccharide-binding protein in murine white adipose tissue [Pubmed:35433160] |
| Siberian Medical Review | Сorrelation between the level of 25 (OH) D3 in blood, the content of antimicrobial peptides in oral fluid and dental caries intensity in young individuals [] |
留言咨询