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淀粉样蛋白β1-42(Ab1-42)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Amyloid Beta Peptide 1-42 (Ab1-42) ,etc. by FLIA (Flow Luminescence Immunoassay)

（注：单次混测多因子不超过8个指标 ）

  • 淀粉样蛋白β1-42(Ab1-42)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 淀粉样蛋白β1-42(Ab1-42)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒对检测淀粉样蛋白β1-42(Ab1-42)等多因子检测试剂盒(流式荧光发光法)具有较高的灵敏度。
经检测小鼠、大鼠之间有100%的交叉反映。

回收率

分别于定值血清及血浆样本中加入一定量的淀粉样蛋白β1-42(Ab1-42)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 80-89 86
EDTA plasma(n=5) 96-105 101
heparin plasma(n=5) 79-90 78

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的淀粉样蛋白β1-42(Ab1-42)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中淀粉样蛋白β1-42(Ab1-42)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 80-95% 98-105% 88-101% 78-104%
EDTA plasma(n=5) 78-89% 89-102% 78-93% 86-99%
heparin plasma(n=5) 85-99% 93-102% 90-98% 90-99%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
    37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将淀粉样蛋白β1-42(Ab1-42)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的淀粉样蛋白β1-42(Ab1-42)等多因子检测试剂盒(流式荧光发光法)和未标记的淀粉样蛋白β1-42(Ab1-42)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的淀粉样蛋白β1-42(Ab1-42)等多因子检测试剂盒(流式荧光发光法)呈负相关。

赠品

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
MAA946Mi21 淀粉样蛋白β1-42(Ab1-42)单克隆抗体 WB; IHC; ICC; IP.
MAA946Mi22 淀粉样蛋白β1-42(Ab1-42)单克隆抗体 WB; IHC; ICC; IP.
CEA946Mi 淀粉样蛋白β1-42(Ab1-42)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA946Mi 淀粉样蛋白β1-42(Ab1-42)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Neuropsychopharmacology Blockade of Tau Hyperphosphorylation and Aβ1–42 Generation by the Aminotetrahydrofuran Derivative ANAVEX2-73, a Mixed Muscarinic and σ1 Receptor Agonist, in a Nontransgenic Mouse Model of Alzheimer’s Disease [Pubmed: 23493042]
Neuropharmacology and analgesia The γ-secretase inhibitor 2-[(1R)-1-[(4-chlorophenyl)sulfonyl](2,5-difluorophenyl) amino]ethyl-5-fluorobenzenebutanoic acid (BMS-299897) alleviates Aβ1–42 seeding and short-term memory deficits in the Aβ25–35 mouse model of Alzheimer's disease [Pubmed: 23123349]
世界科技研究与发展(World Sci-tech R & D) 慢病毒介导的ＣＢＳ基因过表达对原代皮质神经元 [Globesci:Source]
南方医科大学学报 外源性硫化氢对高糖条件下原代神经元β淀粉样蛋白的影响 [J-Smu:Source]
Neurochemical research Effects of Aluminium on β-Amyloid (1–42) and Secretases (APP-Cleaving Enzymes) in Rat Brain [Pubmed:24792732]
Indian J Biochem Biophys. Amyloid ? lowering and cognition enhancing effects of ghrelin receptor analog [D-Lys (3)] GHRP-6 in rat model of obesity [Pubmed:25296496]
Cell Mol Neurobiol Anesthetic Propofol Attenuates Apoptosis, Aβ Accumulation, and Inflammation Induced by Sevoflurane Through NF-κB Pathway in Human Neuroglioma Cells [PubMed: 25809614]
Neuropsychiatr Dis Treat Autophagy dysfunction upregulates beta-amyloid peptides via enhancing the activity of γ-secretase complex [PubMed: 26316755]
Neurochem Int Geniposide attenuates insulin-deficiency-induced acceleration of β-amyloidosis in an APP/PS2 transgenic model of Alzheimer's disease [PubMed: 25882166]
Journal of Alzheimer’s Parkinsonism & Dementia The Potential Effect of Epigallocatechin-3-Gallate alone or in Combination with Vitamin E and Selenium on Alzheimer's Disease Induced by Aluminum in Rats [open-access:]
世界科技研究与发展 芝麻素对高糖条件下PC12细胞Aβ和BACE-1表达的影响 [article:10299]
Am J Alzheimers Dis Other Demen. Tong Luo Jiu Nao, a Chinese Medicine Formula, Reduces Inflammatory Stress in a Mouse Model of Alzheimer's Disease [Pubmed:26993489]
Світ медицини та біології ОСОБЕННОСТИ ПРОЦЕССОВ ФОРМИРОВАНИЯ ПОКАЗАТЕЛЕЙ ФУНКЦИОНАЛЬНОГО СОСТОЯНИЯ ВЫСШЕЙ НЕРВНОЙ ДЕЯТЕЛЬНОСТИ И ХАРАКТЕРИСТИКИ ВНИМАНИЯ СТУДЕНТОВ [SMB-2016-03-062.pdf]
世界科技研究与发展 芝麻素对高糖条件下PC12 细胞Aß 和BACE 1 表达的影响 [QK:92621A]
Archives of Pharmacal Research Geniposide attenuates the level of Aβ1–42 via enhancing leptin signaling in cellular and APP/PS1 transgenic mice [pubmed:28160136]
Food Funct. Effects of dietary glucocerebrosides from sea cucumber on the brain sphingolipid profiles of mouse models of Alzheimer's disease. [pubmed:28232989]
Scientific Reports Norovirus P particle-based active Aβ immunotherapy elicits sufficient immunogenicity and improves cognitive capacity in a mouse model of Alzheimer’s disease [articles:srep41041.ris]
Journal of Medicinal Food Cerebrosides from Sea Cucumber Protect Against Oxidative Stress in SAMP8 Mice and PC12 Cells [pubmed:28406733]
Journal of Nutritional Biochemistry Mechanisms of DHA-enriched phospholipids in improving cognitive deficits in aged SAMP8 mice with high-fat diet [Pubmed:29986309]
Biochemical Pharmacology Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in naïve and Aβ-treated female rats [Pubmed:30028991]
Journal of Affective Disorders Sub-chronic celecoxib prevents soluble beta amyloid-induced depressive-like behaviour in rats [Pubmed:29879605]
Biomedicine & Pharmacotherapy Punicalagin ameliorates the elevation of plasma homocysteine, amyloid-β, TNF-α and apoptosis by advocating antioxidants and modulating apoptotic mediator proteins in brain modulating apoptotic mediator … [Pubmed:29579708]
Journal of Agricultural and Food Chemistry Effects of Astaxanthin and Docosahexaenoic-Acid-Acylated Astaxanthin on Alzheimer's Disease in APP/PS1 Double-Transgenic Mice [Pubmed:29695154]
Neurotoxicology Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in rats [Pubmed:29522778]
Biochemical and Biophysical Research Communications CART peptide activates the Nrf2/HO-1 antioxidant pathway and protects hippocampal neurons in a rat model of Alzheimer's disease [Pubmed:29777699]
Oncotarget Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse … [Pubmed:29731975]
Experimental and Therapeutic Medicine Ellagic acid ameliorates learning and memory impairment in APP/PS1 transgenic mice via inhibition of β‑amyloid production and tau hyperphosphorylation [Doi: 10.3892/etm.2018.6860]
非编码RNA miR-137 调控tau 蛋白磷酸化和L 型钙离子通道CACNA1C 参与阿尔茨海默病的发生 []
台湾农业研究 (台灣農業研究67 (4): 355-364) 香菇柄水萃物改善糖尿病降低阿茲海默氏症罹病風險初探 []
The Efficacy of Sauropus androgynus Leaves Extract To Improve Cognitive Function in Wistar Rats Induced Alzheimer's []
Neurotoxicity Research Effect of Aluminum-Maltolate on the Content of Aβ Protein and the Expression of ApoER2, VLDLRs, and LRP1 in PC12-ApoE4 Cells [Pubmed: 30649678]
Neurochemical Research Imbalance of Microglial TLR4/TREM2 in LPS-Treated APP/PS1 Transgenic Mice: A Potential Link Between Alzheimer's Disease and Systemic Inflammation [Pubmed: 30756214]
Molecular and Cellular Biochemistry Repetitive transcranial magnetic stimulation protects mice against 6-OHDA-induced Parkinson's disease symptoms by regulating brain amyloid β level []
PLoS One Obstructive sleep apnea may increase the risk of Alzheimer's disease [Pubmed: 31487291]
Metabolic Brain Disease Nanoformulated ellagic acid ameliorates pentylenetetrazol-induced experimental epileptic seizures by modulating oxidative stress, inflammatory cytokines and … [Pubmed: 31728888]
博士论文 Neurobiological determinants of depressive-like symptoms in rodents []
Pharmacognosy Magazine Mahanimbine-induced neuroprotection via cholinergic system and attenuated amyloidogenesis as well as neuroinflammation in lipopolysaccharides-induced … []
biosciences biotechnology research asia Dietary Malted Barley Grain Improved Structure and Function of Spinal Cord of Mother Rats fed on a high cholesterol diet. []
Neurochem Res Ex Vivo Investigation of Bexarotene and Nicotinamide Function as a Protect?ve Agent on Rat Synaptosomes Treated with A¦Â (1-42) [33428094]
Food Funct Comparative study on the effects of phosphatidylserine rich in DHA and EPA on A¦Â-intervened Alzheimer's disease using cell models [33876786]
Int J Neurosci Protective effects of repetitive transcranial magnetic stimulation against 6-OHDA-induced Parkinson's symptoms in a mice model: the key role of miR-409-3p/PDHB ¡­ [34078216]
Brain Res Bul Immunization with A¦Â3-10-KLH vaccine improves cognitive function and ameliorates mitochondrial dysfunction and reduces Alzheimer's disease-like pathology in Tg ¡­ [34044034]
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS The effect of a 6-month Ketogenic Medium-Chain Triglyceride Supplement on Plasma Cardiometabolic and Inflammatory Markers in Mild Cognitive?¡­ [33906081]
Crystals Potential Therapeutic Effects of New Ruthenium (III) Complex with Quercetin: Characterization, Structure, Gene Regulation, and Antitumor and Anti-Inflammatory Studies (RuIII/Q Novel Complex Is a Potent Immunoprotective Agent) []
Neurosci Lett GPNMB mitigates Alzheimer's disease and enhances autophagy via suppressing the mTOR signal [34695452]
Arch Gerontol Geriatr The influence of vinpocetine alone or in combination with Epigallocatechin-3-gallate, Coenzyme COQ10, Vitamin E and Selenium as a potential neuroprotective … [34706318]
Metab Brain Dis Probiotics ameliorates LPS induced neuroinflammation injury on Aβ 1–42, APP, γ-β secretase and BDNF levels in maternal gut microbiota and fetal … [Pubmed:35312928]
Medicinal Chemistry Research New Thienopyridine Scaffold as An Antidementia Lead: In Silico Study and Biological Screening []
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