心钠肽(ANP)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Atrial Natriuretic Peptide (ANP) ,etc. by FLIA (Flow Luminescence Immunoassay)
ANH; ANF; CDD; Atrial Natriuretic Factor; Atrial Natriuretic Hormone; Atriopeptin; Cardionatrine; Cardiodilatin
（注：单次混测多因子不超过8个指标 ）
- 编号LMA225Ca
- 物种Canis familiaris; Canine (Dog，犬) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围0.98-1000pg/mL
- 灵敏度最小可检测剂量小于等于0.327 pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
- 价格 ¥ 2696 ¥ 2799 ¥ 2955 ¥ 3162 ¥ 3370 ¥ 3681 ¥ 4147 ¥ 5184 添加到价格计算器 计算器
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测心钠肽(ANP)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的心钠肽(ANP)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 89-103 | 95 |
| EDTA plasma(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 79-103 | 95 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的心钠肽(ANP)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中心钠肽(ANP)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-101% | 85-102% | 78-93% | 92-104% |
| EDTA plasma(n=5) | 79-94% | 80-101% | 87-94% | 97-105% |
| heparin plasma(n=5) | 83-102% | 89-103% | 91-101% | 80-98% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将心钠肽(ANP)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的心钠肽(ANP)等多因子检测试剂盒(流式荧光发光法)和未标记的心钠肽(ANP)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的心钠肽(ANP)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Canis familiaris; Canine (Dog，犬) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA225Ca01 | 心钠肽(ANP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA225Ca21 | 心钠肽(ANP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA225Ca01 | 心钠肽(ANP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA225Ca08 | 心钠肽(ANP)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA225Ca21 | 心钠肽(ANP)单克隆抗体 | WB; IHC; ICC; IP. |
| CEA225Ca | 心钠肽(ANP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA225Ca | 心钠肽(ANP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| 8 | Volume Status and Diuretic Therapy in Systolic Heart Failure and the Detection of Early Abnormalities in Renal and Tubular Function [PubMed: 21616283] |
| Basic Research in Cardiology | Release of atrial natriuretic peptide precedes shedding of the endothelial glycocalyx equally in patients undergoing on- and off-pump coronary artery bypass surgery [PubMed: 21769675] |
| Neuropsychobiology. | Atrial natriuretic Peptide, arginine vasopressin Peptide and cortisol serum levels in opiate-dependent patients [PubMed: 23406607] |
| European addiction research | Alcohol-induced changes in methylation status of individual CpG sites, and serum levels of vasopressin and atrial natriuretic peptide in alcohol-dependent patients during detoxification treatment. [Pubmed: 24356727] |
| Crit Care. | Hypervolemia increases release of atrial natriuretic peptide and shedding of the endothelial glycocalyx [Pubmed:25497357] |
| Diabet Med. | Sodium-glucose co-transporter-2 inhibitors suppress atrial natriuretic peptide secretion in patients with newly diagnosed Type 2 diabetes. [Pubmed:26927445] |
| J Ethnopharmacol | Danhong injection attenuates isoproterenol-induced cardiac hypertrophy by reCavia (Guinea pig )lating p38 and NF-κb pathway [Pubmed:26970569] |
| Journal of Babylon University/Pure and Applied Sciences | Blood Pressure and Atrial Natriuretic Peptide (ANP) Levels in Patients with Autosomal Dominant Polycystic Kidney Disease. [papers:uobj_paper_2016_62613672.pdf] |
| PLoS One | Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy [pubmed:29240788] |
| Drug Delivery | Ginsenoside Rg3 micelles mitigate doxorubicin-induced cardiotoxicity and enhance its anticancer efficacy. [pubmed:29063791] |
| Elife | SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation [Pubmed:29504933] |
| Science of the Total Environment | Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and [Pubmed:29727991] |
| The Journal of Biological Chemistry | Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice [Pubmed:29929978] |
| Pediatric Cardiology | Determinants of Increased Aortic Diameters in Young Normotensive Patients With Turner Syndrome Without Structural Heart Disease [Pubmed:29392348] |
| FASEB Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure [Pubmed: 30230922] |
| Endokrynologia Polska | Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension … [Pubmed: 30359461] |
| International Journal of Peptide Research and Therapeutics | ANP/NPRA Inhibits Epithelial-Mesenchymal Transition of Airway by Targeting Smad3 in Asthma [] |
| Van Veterinary Journal | Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure. [] |
| Acta Pharmaceutica Sinica B | The poly (ADP-ribosyl) ation of BRD4 mediated by PARP1 promoted pathological cardiac hypertrophy [34094834] |
| Peptides | Alarin moderated myocardial hypertrophy via inhibiting cyclic adenosine monophosphate/protein kinase A signaling pathway to attenuate autophagy [34624429] |
| Peptides | Osteocrin alleviates cardiac hypertrophy via attenuating oxidative stress [Pubmed:35218795] |