胰高血糖素样肽1(GLP1)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Glucagon Like Peptide 1 (GLP1) ,etc. by FLIA (Flow Luminescence Immunoassay)
（注：单次混测多因子不超过8个指标 ）
- 编号LMA804Mi
- 物种Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Oryctolagus cuniculus (Rabbit，兔), Canis familiaris; Canine (Dog，犬), Bos taurus; Bovine (Cattle，牛) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围0.98-1000pg/mL
- 灵敏度最小可检测剂量小于等于0.327 pg/mL.
- 样本类型Serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒对检测胰高血糖素样肽1(GLP1)等多因子检测试剂盒(流式荧光发光法)具有较高的灵敏度。
经检测人、小鼠、大鼠、兔、犬、牛之间有100%的交叉反映。
回收率
分别于定值血清及血浆样本中加入一定量的胰高血糖素样肽1(GLP1)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 91-101 | 98 |
| EDTA plasma(n=5) | 84-93 | 89 |
| heparin plasma(n=5) | 92-105 | 101 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的胰高血糖素样肽1(GLP1)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中胰高血糖素样肽1(GLP1)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-91% | 78-101% | 95-103% | 96-105% |
| EDTA plasma(n=5) | 91-98% | 98-105% | 86-97% | 95-105% |
| heparin plasma(n=5) | 93-101% | 98-105% | 78-101% | 91-98% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将胰高血糖素样肽1(GLP1)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的胰高血糖素样肽1(GLP1)等多因子检测试剂盒(流式荧光发光法)和未标记的胰高血糖素样肽1(GLP1)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的胰高血糖素样肽1(GLP1)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Oryctolagus cuniculus (Rabbit，兔), Canis familiaris; Canine (Dog，犬), Bos taurus; Bovine (Cattle，牛) | 应用(仅供研究使用，不用于临床诊断！) |
| CPA804Mi22 | 胰高血糖素样肽1(GLP1)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA804Mi21 | 胰高血糖素样肽1(GLP1)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA804Mi01 | 胰高血糖素样肽1(GLP1)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA804Mi21 | 胰高血糖素样肽1(GLP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA804Mi22 | 胰高血糖素样肽1(GLP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA804Mi24 | 胰高血糖素样肽1(GLP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MEA804Mi | 胰高血糖素样肽1(GLP1)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA804Mi | 胰高血糖素样肽1(GLP1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA804Mi | 胰高血糖素样肽1(GLP1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
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| Acta Histochemica | Expression of cholecystokinin2-receptor in rat and human L cells and the stimulation of glucagon-like peptide-1 secretion by gastrin treatment [Pubmed:25601282] |
| Journal of Endocrinology | Inhibitory effect of somatostatin on insulin secretion is not mediated via the CNS [Pubmed:25630331] |
| Obes Surg | Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat Model [PubMed: 26202420] |
| International Journal of Pharmacognosy and Phytochemical Research | Anti-hiperglycemic Effect of Urena lobata Leaf Extract by Inhibition of Dipeptidyl Peptidase IV (DPP-IV) on Diabetic Rats [Pdf: 7] |
| European Journal of Pharmacology | Subcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse model [Pubmed:27131827] |
| Int J Obes | miR-200a reCavia (Guinea pig )lates Rheb-mediated amelioration of insulin resistance after duodenal-jejunal bypass [Pubmed:27121251] |
| Journal of Traditional and Complementary Medicine | Incretin effect of Urena lobata leaves extract on structure and function of rats islet β-cells [S2225411016302802] |
| World Journal of Gastroenterology | Alterations in gut microbiota during remission and recurrence of diabetes after duodenal-jejunal bypass in rats [pubmed:27547013] |
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| Journal of Physiology and Biochemistry | Downregulation of lncRNA MALAT1 contributes to renal functional improvement after duodenal-jejunal bypass in a diabetic rat model [Pubmed:29781038] |
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| Nutrition in the Prevention and Treatment of Abdominal Obesity | Effects of Diet-Induced Early-Stage Obesity on a Low-Testosterone Gottingen Minipig [] |
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