谷胱甘肽还原酶(GR)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Glutathione Reductase (GR) ,etc. by FLIA (Flow Luminescence Immunoassay)
GSR; GLUR; GRD1; Glutathione reductase, mitochondrial
(注:单次混测多因子不超过8个指标 )
- 编号LMB314Ra
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.01-10ng/mL
- 灵敏度最小可检测剂量小于等于0.003 ng/mL.
- 样本类型Serum, plasma, tissue homogenates and other biological fluids
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特异性
本试剂盒用于检测谷胱甘肽还原酶(GR)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的谷胱甘肽还原酶(GR)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 87-96 | 91 |
EDTA plasma(n=5) | 83-90 | 86 |
heparin plasma(n=5) | 95-102 | 99 |
sodium citrate plasma(n=5) | 85-93 | 88 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的谷胱甘肽还原酶(GR)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中谷胱甘肽还原酶(GR)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 88-96% | 96-103% | 85-99% | 79-90% |
EDTA plasma(n=5) | 79-98% | 96-104% | 85-98% | 94-105% |
heparin plasma(n=5) | 88-96% | 91-98% | 84-96% | 95-102% |
sodium citrate plasma(n=5) | 89-97% | 94-105% | 79-97% | 82-92% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠)标准品或样本100μL及磁珠10μL,
37°C酶标板振荡器孵育90分钟;
3. 磁吸甩干,加检测溶液A100μL,37°C酶标板振荡器孵育60分钟;
4. 磁吸洗板3次;
5. 加检测溶液B100μL,37°C振动孵育30分钟;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋涡震荡2分钟后读数。
实验原理
将谷胱甘肽还原酶(GR)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠,其中的谷胱甘肽还原酶(GR)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合,然后加入生物素化的谷胱甘肽还原酶(GR)等多因子检测试剂盒(流式荧光发光法)抗体,将未结合的生物素化抗体洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的谷胱甘肽还原酶(GR)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPB314Ra01 | 谷胱甘肽还原酶(GR)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB314Ra01 | 谷胱甘肽还原酶(GR)多克隆抗体 | WB; IHC; ICC; IP. |
MAB314Ra21 | 谷胱甘肽还原酶(GR)单克隆抗体 | WB; IHC; ICC; IP. |
SEB314Ra | 谷胱甘肽还原酶(GR)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB314Ra | 谷胱甘肽还原酶(GR)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
Oxidative Medicine and Cellular Longevity | Doxorubicin Toxicity can be Ameliorated during Antioxidant L-Carnitine Supplementation [PubMed: PMC3154045] |
PLoS One. | P2×7?Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine Supplementation [Plos:Source] |
Diabetes/Metabolism Research and Reviews | Reduced peripheral blood mtDNA content is associated with impaired glucose‐stimulated islet β cell function in a Chinese population with different degrees of … [doi:10.1002] |
Nutrition Journal | Influence of diet on leukocyte telomere length, markers of inflammation and oxidative stress in individuals with varied glucose tolerance: a Chinese population study [Pubmed:27071648] |
Pharmacological reports | The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorder [science:article] |
Pharmacological Reports | The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder. [pubmed:28623707] |
Canadian journal of physiology and pharmacology | Ambroxol attenuates cisplatin-induced hepato and nephrotoxicity via inhibition of p-JNK/p-ERK [Pubmed: 30383980] |
FREE RADICAL RESEARCH | Negative association between antioxidant vitamin intake and non-alcoholic fatty liver disease in Chinese non-diabetic adults: mediation models involving superoxide … [Pubmed: 32985285] |
Lipids Health Dis | Triglyceride is independently correlated with insulin resistance and islet beta cell function: a study in population with different glucose and lipid metabolism … [Pubmed: 32487177] |
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY | Potential Protective Effect of Dietary Intake of Non-α-Tocopherols on Cellular Aging Markers Mediated by Tumor Necrosis Factor-α in Prediabetes: A Cross … [Pubmed: 32509152] |
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY | TNFα Mediates the Interaction of Telomeres and Mitochondria Induced by Hyperglycemia: A Rural Community-Based Cross-Sectional Study [Pubmed: 32454945] |
oxidative medine cellular longevity | Relationship between Decreased Serum Superoxide Dismutase Activity and Metabolic Syndrome: Synergistic Mediating Role of Insulin Resistance and β … [Pubmed: 32617139] |
The Journal of Clinical Endocrinology & Metabolism | High Hemoglobin glycation index is associated with telomere attrition independent of HbA1c, mediating by TNFα [34562085] |