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免疫球蛋白A(IgA)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Immunoglobulin A (IgA) ,etc. by FLIA (Flow Luminescence Immunoassay)

(注:单次混测多因子不超过8个指标 )

  • 免疫球蛋白A(IgA)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 免疫球蛋白A(IgA)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测免疫球蛋白A(IgA)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的免疫球蛋白A(IgA)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 97-104 101
EDTA plasma(n=5) 83-99 92
heparin plasma(n=5) 92-99 95
sodium citrate plasma(n=5) 84-105 91

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的免疫球蛋白A(IgA)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中免疫球蛋白A(IgA)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 90-101% 80-96% 78-92% 94-103%
EDTA plasma(n=5) 88-95% 89-103% 97-105% 92-99%
heparin plasma(n=5) 84-94% 94-105% 98-105% 96-105%
sodium citrate plasma(n=5) 89-96% 95-104% 93-105% 83-101%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠)标准品或样本100μL及磁珠10μL,
    37°C酶标板振荡器孵育90分钟;
3. 磁吸甩干,加检测溶液A100μL,37°C酶标板振荡器孵育60分钟;
4. 磁吸洗板3次;
5. 加检测溶液B100μL,37°C振动孵育30分钟;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋涡震荡2分钟后读数。

实验原理

将免疫球蛋白A(IgA)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠,其中的免疫球蛋白A(IgA)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合,然后加入生物素化的免疫球蛋白A(IgA)等多因子检测试剂盒(流式荧光发光法)抗体,将未结合的生物素化抗体洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的免疫球蛋白A(IgA)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

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LMA546Bo 免疫球蛋白A(IgA)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
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参考文献

杂志 参考文献
IDOSI Publications Sequence-Based Typing-Study on the Relationship Between Subclinical Mastitis and BoLA-DRB3.2* Allelic Polymorphism in Egyptian Cows [Idosi: Source]
international Journal of food and nutritional sciences EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI [Ijfans: Source]
Poultry Science Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 [Pubmed: 24570422 ]
Clinical and Vaccine Immunology Specific humoral immune response induced by Propionibacterium acnes can prevent Actinobacillus pleuropneumoniae infection in mice [Asm: Source]
Clin Vaccine Immunol. Speci?c Humoral Immune Response Induced by Propionibacterium acnes Can Prevent Actinobacillus pleuropneumoniae Infection in Mice [Pubmed:24429068]
Journal of Proteomics Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infection [Pubmed:24576640]
Food & Function Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice [Pubmed:25308407]
Pediatrics Oropharyngeal colostrum administration in extremely premature infants: an RCT. [Pubmed:25624376]
Front Microbiol Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis [PubMed: 26217323]
Food and Agricultural Immunology Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers [10.108:09540105.2017.1311308 ]
SYSTEMISCHE ENTZÜNDUNG BEI CHRONISCHER NIERENINSUFFIZIENZ – DIE ROLLE DER DARMFLORA [patent:US9763998B2]
Water Research  Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model [Pubmed:29655095]
Vaccine Recombinant Lactococcus lactis co-expressing OmpH of an M cell-targeting ligand and IBDV-VP2 protein provide immunological protection in chickens [Pubmed:29289381]
Food and Agricultural Immunology Effects of and on growth performance, immune function and volatile fatty acid level of caecal digesta in broilers [10.1080:09540105.2018.1457013]
Probiotics Antimicrob Proteins Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a … [Pubmed:29305686]
SAGE Open Medicine Altered immunoglobulins (A and G) in Ghanaian patients with type 2 diabetes [Pubmed:29623201]
Nephrology Dialysis Transplantation The gut flora modulates intestinal barrier integrity but not progression of chronic kidney disease in hyperoxaluria-related nephrocalcinosis [Pubmed: 31081025]
Current Developments in Nutrition Yacon (Smallanthus sonchifolius): Effect on Integrity of Intestinal Barrier, Inflammatory Response and Oxidative Stress in Animal Model of Colon Cancer (P06-052-19) [Pubmed: 31225040]
Nutrients. Effects of Paramylon Extracted from Euglena gracilis EOD-1 on Parameters Related to Metabolic Syndrome in Diet-Induced Obese Mice [Pubmed: 31330894]
International Journal of Biological Macromolecules Effects of polysaccharides from Yingshan Yunwu tea on meat quality, immune status and intestinal microflora in chickens [Pubmed: 32224178]
Effect Long-Term Exposure to Different Doses of Lipopolysaccharides on the Intestinal Mucosal Immune Barrier []
Italian Journal of Animal Science Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the … []
博士论文 Women Scientist Scientist Mentor []
Acta Paediatr MicroRNA profiling in Chinese children with Henoch©\Schonlein purpura and association between selected microRNAs and inflammatory biomarkers [33533510]
Environ Pollut Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure [34090073]
J Ethnopharmacol Monascus ruber fermented Panax ginseng ameliorates lipid metabolism disorders and modulate gut microbiota in rats fed a high-fat diet [34098018]
Microbiology Spectrum Dietary Supplementation of Inulin Ameliorates Subclinical Mastitis via Regulation of Rumen Microbial Community and Metabolites in Dairy Cows [34494854]
Food Research International Sulfated modification enhances the immunomodulatory effect of Cyclocarya paliurus polysaccharide on cyclophosphamide-induced immunosuppressed mice through … [34865774]
Food Funct Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components [Pubmed:35438093]
Food and Agricultural Immunology The effect of the multi-strain probiotic preparation EM Bokashi® on selected parameters of the cellular immune response in pigs []
Journal of Functional Foods Bovine lactoferricin ameliorates intestinal inflammation and mucosal barrier lesions in colitis through NF-κB/NLRP3 signaling pathways []
Journal of Dentistr Journal of Dentistry Indonesia Salivary IgA Depression in Drug-Influenced Gingival Enlargement among Hypertensive Patients []
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