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干扰素γ(IFNg)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Interferon Gamma (IFNg) ,etc. by FLIA (Flow Luminescence Immunoassay)

IFN-G; IFG; IFI; INFr; IFN, Immune Interferon

(注:单次混测多因子不超过8个指标 )

  • 干扰素γ(IFNg)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 干扰素γ(IFNg)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测干扰素γ(IFNg)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的干扰素γ(IFNg)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 98-105 101
EDTA plasma(n=5) 80-98 88
heparin plasma(n=5) 79-103 96

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的干扰素γ(IFNg)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中干扰素γ(IFNg)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 78-104% 87-101% 78-98% 84-93%
EDTA plasma(n=5) 92-99% 85-99% 79-102% 95-103%
heparin plasma(n=5) 90-98% 93-105% 99-105% 90-98%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠)标准品或样本100μL及磁珠10μL,
    37°C酶标板振荡器孵育90分钟;
3. 磁吸甩干,加检测溶液A100μL,37°C酶标板振荡器孵育60分钟;
4. 磁吸洗板3次;
5. 加检测溶液B100μL,37°C振动孵育30分钟;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋涡震荡2分钟后读数。

实验原理

将干扰素γ(IFNg)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠,其中的干扰素γ(IFNg)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合,然后加入生物素化的干扰素γ(IFNg)等多因子检测试剂盒(流式荧光发光法)抗体,将未结合的生物素化抗体洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的干扰素γ(IFNg)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种:Sus scrofa; Porcine (Pig,猪) 应用(仅供研究使用,不用于临床诊断!)
EPA049Po61 干扰素γ(IFNg)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA049Po61 干扰素γ(IFNg)活性蛋白 Cell culture; Activity Assays.
RPA049Po01 干扰素γ(IFNg)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA049Po01 干扰素γ(IFNg)多克隆抗体 WB; IHC; ICC; IP.
PAA049Po06 干扰素γ(IFNg)多克隆抗体 WB; IHC; ICC; IP.
MAA049Po21 干扰素γ(IFNg)单克隆抗体 WB; IHC; ICC; IP.
SEA049Po 干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA049Po 干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA049Po 干扰素γ(IFNg)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA049Po 干扰素γ(IFNg)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA049Po01 干扰素γ(IFNg)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

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Int Immunopharmacol Celastrol attenuates collagen-induced arthritis via inhibiting oxidative stress in rats [Pubmed: 32402948]
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Journal of Functional Foods Polyphenolic extracts from Wushan tea leaves attenuate hepatic injury in CCl4-treated mice []
Indian Journal of Animal Research Immunological Effects of a Single Dose of PLGA Nanoparticles Encapsulated Peptide in Broilers in Comparison to Traditional Vaccines against Infectious Bursal … []
Hormonal and Cytokine Blood Profile of Dairy Cows in the Early Gestation Period []
Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction by enhancing Th2 cytokine []
Scientific Reports Immunogenicity and protective efficacy of enterotoxigenic Escherichia coli (ETEC) total RNA against ETEC challenge in a mouse model [33239756]
Animal Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512]
Eur Rev Med Pharmacol Sci Salivary Irisin: potential inflammatory biomarker in recurrent apthous stomatitis patients [33755963]
Biomed Pharmacother Macro- and microtranscriptomic evidence of the monocyte recruitment to regenerating liver after partial hepatectomy in mouse model [33765583]
PLoS One Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies [33999928]
Life Sciences p-Coumaric acid ameliorates ionizing radiation-induced intestinal injury through modulation of oxidative stress and pyroptosis [33915129]
Infect Drug Resist Talaromyces marneffei and Burkholderia cepacia Co-Infection in a HIV-Uninfected Patient with Anti-Interferon-¦Ã Autoantibodies [34140785]
Vaccines Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious?¡­ [33670249]
Pharmaceutics PK/PD Modeling of the PDE7 Inhibitor¡ªGRMS-55 in a Mouse Model of Autoimmune Hepatitis. Pharmaceutics 2021, 13, 597 [33919375]
Melatonin-mediated MT2 attenuates colitis induced by dextran sodium sulfate via PI3K/AKT/Nrf2/SIRT1/ROR¦Á/NF-¦ÊB signaling pathways [34162146]
How Does the Th1/Th2 Polarization in Milk Change According to the Types of Bacteria that Cause Subclinical Mastitis in Cows? []
Amplified Antitumor Efficacy By A Targeted Drugs Retention and Chemosensitization Strategies-Based ¡°Combo¡± Nanoagent Together With PD-L1 Blockade In?¡­ []
World J Tradit Chin Med Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction via Th2 cytokine enhancement []
Cancer Lett Photodynamic therapy induces human esophageal carcinoma cell pyroptosis by targeting the PKM2/caspase-8/caspase-3/GSDME axis [34256094]
J Pharm Pharmacol Alamandine protects against renal ischaemia–reperfusion injury in rats via inhibiting oxidative stress [34244746]
J Nanobiotechnology Amplified antitumor efficacy by a targeted drug retention and chemosensitization strategy-based “combo” nanoagent together with PD-L1 blockade in … [34225744]
Neoplasia PDIA6 promotes pancreatic cancer progression and immune escape through CSN5-mediated deubiquitination of β-catenin and PD-L1 [34325342]
Sci Rep Talaromyces marneffei and nontuberculous mycobacteria co-infection in HIV-negative patients [34376749]
Am J Transl Res Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma [34377237]
J Immunol Res Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus [34395633]
Sci Rep Novel interferon-sensitive genes unveiled by correlation-driven gene selection and systems biology [34508139]
Transl Psychiatry Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive … [34802039]
Infect Drug Resist Retrospective Analysis of 10 Cases of Disseminated Nontuberculous Mycobacterial Disease with Osteolytic Lesions [34785914]
Environmental Research In-vitro oxidative potential and inflammatory response of ambient PM2. 5 in a rural region of Northwest China: Association with chemical compositions and … [34863982]
Eur J Pharm Sci Preclinical study of methotrexate-based hydrogels versus surfactant based liquid crystal systems on psoriasis treatment [34314841]
J Inflamm Res Clenbuterol, a Selective β2-Adrenergic Receptor Agonist, Inhibits or Limits Post-Stroke Pneumonia, but Increases Infarct Volume in MCAO Mice [Pubmed:35058704]
Poult Sci Salmonella phage CKT1 significantly relieves the body weight loss of chicks by normalizing the abnormal intestinal microbiome caused by hypervirulent … [Pubmed:35063807]
ACS Appl Mater Interfaces Combinational Chemoimmunotherapy for Breast Cancer by Codelivery of Doxorubicin and PD-L1 siRNA Using a PAMAM-Incorporated Liposomal Nanoplatform [Pubmed:35138103]
Theranostics Combating multidrug resistance and metastasis of breast cancer by endoplasmic reticulum stress and cell-nucleus penetration enhanced … [Pubmed:35401832]
Inflammopharmacology Silymarin constrains diacetyl-prompted oxidative stress and neuroinflammation in rats: involvements of Dyn/GDNF and MAPK signaling pathway [Pubmed:35366745]
Journal of Nanobiotechnology Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM [Pubmed:35351131]
Infection and Drug Resistance Sweet's Syndrome Accompanied by Coinfection with Multiple Pathogens and Disseminated Mycobacterium phlei Infection Presenting with Osteolytic … [Pubmed:35592103]
Sci Rep Clinical findings and predictive factors for positive anti-interferon-γ autoantibodies in patients suffering from a non-tuberculosis mycobacteria or Talaromyces … [Pubmed:35641599]
Biomedicines Deletion of Toxoplasma Rhoptry Protein 38 (PruΔrop38) as a Vaccine Candidate for Toxoplasmosis in a Murine Model [Pubmed:35740356]
Viruses Synergistic Pathogenicity by Coinfection and Sequential Infection with NADC30-like PRRSV and PCV2 in Post-Weaned Pigs [Pubmed:35215787]
Antioxidants Blue Light Alters the Composition of the Jejunal Microbiota and Promotes the Development of the Small Intestine by Reducing Oxidative Stress [Pubmed:35204158]
Oxidative Medicine and Cellular Longevity The Role of Aeromonas-Goblet Cell Interactions in Melatonin-Mediated Improvements in Sleep Deprivation-Induced Colitis [Pubmed:35355860]
Journal of Integrative Medicine Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats [Pubmed:35643766]
researchsquare MSC-EVs: A Promising Modulator for Immune Response in Systemic Lupus Erythematosus []
Mljekarstvo Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows []
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