干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法,小样本)
Mini Samples ELISA Kit for Interferon Gamma (IFNg)
IFN-G; IFG; IFI; INFr; IFN, Immune Interferon
- 编号MEA049Po
- 物种Sus scrofa; Porcine (Pig，猪) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围15.6-1,000pg/mL
- 灵敏度最小可检测剂量小于等于6.2pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2260 ¥ 3228 ¥ 14526 ¥ 27438 ¥ 225960
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法,小样本)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法,小样本)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 99-105 | 102 |
| EDTA plasma(n=5) | 80-94 | 89 |
| heparin plasma(n=5) | 84-104 | 95 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法,小样本)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法,小样本)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-97% | 89-97% | 82-93% | 80-97% |
| EDTA plasma(n=5) | 79-95% | 85-102% | 98-105% | 91-105% |
| heparin plasma(n=5) | 93-101% | 92-101% | 85-101% | 78-91% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）25µL，37°C温育1小时；
3. 吸弃，加检测溶液A25µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B25µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物25µL，37°C孵育10-20分钟；
8. 加终止液20µL，立即450nm读数。
实验原理
将干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法,小样本)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法,小样本)与连接于固相载体上的抗体结合，然后加入生物素化的干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法,小样本)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法,小样本)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Sus scrofa; Porcine (Pig，猪) | 应用(仅供研究使用，不用于临床诊断！) |
| EPA049Po61 | 干扰素γ(IFNg)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA049Po61 | 干扰素γ(IFNg)活性蛋白 | Cell culture; Activity Assays. |
| RPA049Po01 | 干扰素γ(IFNg)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA049Po01 | 干扰素γ(IFNg)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA049Po06 | 干扰素γ(IFNg)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA049Po21 | 干扰素γ(IFNg)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA049Po | 干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA049Po | 干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA049Po | 干扰素γ(IFNg)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA049Po | 干扰素γ(IFNg)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA049Po01 | 干扰素γ(IFNg)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
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| BIOMEDICINE & PHARMACOTHERAPY | Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress [Pubmed: 33022535] |
| DRUG AND CHEMICAL TOXICOLOGY | Toxicological impact of sodium benzoate on inflammatory cytokines, oxidative stress and biochemical markers in male Wistar rats [Pubmed: 33003960] |
| Life Sci | PIGU promotes hepatocellular carcinoma progression through activating NF-κB pathway and increasing immune escape [Pubmed: 32971102] |
| Vet Res | Construction of polycistronic baculovirus surface display vectors to express the PCV2 Cap (d41) protein and analysis of its immunogenicity in mice and swine [Pubmed: 32907618] |
| Int Immunopharmacol | BNIP3 deletion ameliorated enterovirus 71 infection-induced hand, foot and mouth disease via inhibiting apoptosis, autophagy, and inflammation in mice [Pubmed: 32717566] |
| EXPERIMENTAL DERMATOLOGY | Decreased suppression of CD8+ and CD4+ T cells by peripheral Regulatory T cells in Generalized Vitiligo due to reduced NFATC1 and FOXP3 proteins [Pubmed: 32682346] |
| Parasites & Vectors | Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism … [Pubmed: 32680553] |
| Life Sci | Impact of diminazene aceturate on renin-angiotensin system, infectious myocarditis and skeletal myositis in mice: An in vitro and in vivo study [Pubmed: 32652140] |
| cancer immunology immunotherapy | TIM-3 blockade combined with bispecific antibody MT110 enhances the anti-tumor effect of γδ T cells [Pubmed: 32588076] |
| BIOLOGICAL TRACE ELEMENT RESEARCH | Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle [Pubmed: 32583225] |
| Molecular Medicine Reports | Aquaporin‑4 deletion ameliorates enterovirus 71 infection in mice [Pubmed: 32582978] |
| BIOTECHNOLOGY LETTERS | Recombinant ferritin nanoparticles can induce dendritic cell maturation through TLR4/NF-κB pathway [Pubmed: 32567013] |
| Int Immunopharmacol | Celastrol attenuates collagen-induced arthritis via inhibiting oxidative stress in rats [Pubmed: 32402948] |
| Evaluation of lipopolysaccharide-induced acute lung injury attenuation in mice by Glycyrrhiza glabra [] | |
| Journal of Functional Foods | Polyphenolic extracts from Wushan tea leaves attenuate hepatic injury in CCl4-treated mice [] |
| Indian Journal of Animal Research | Immunological Effects of a Single Dose of PLGA Nanoparticles Encapsulated Peptide in Broilers in Comparison to Traditional Vaccines against Infectious Bursal … [] |
| Hormonal and Cytokine Blood Profile of Dairy Cows in the Early Gestation Period [] | |
| Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction by enhancing Th2 cytokine [] | |
| Scientific Reports | Immunogenicity and protective efficacy of enterotoxigenic Escherichia coli (ETEC) total RNA against ETEC challenge in a mouse model [33239756] |
| Animal | Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512] |
| Eur Rev Med Pharmacol Sci | Salivary Irisin: potential inflammatory biomarker in recurrent apthous stomatitis patients [33755963] |
| Biomed Pharmacother | Macro- and microtranscriptomic evidence of the monocyte recruitment to regenerating liver after partial hepatectomy in mouse model [33765583] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies [33999928] |
| Life Sciences | p-Coumaric acid ameliorates ionizing radiation-induced intestinal injury through modulation of oxidative stress and pyroptosis [33915129] |
| Infect Drug Resist | Talaromyces marneffei and Burkholderia cepacia Co-Infection in a HIV-Uninfected Patient with Anti-Interferon-¦Ã Autoantibodies [34140785] |
| Vaccines | Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious?¡ [33670249] |
| Pharmaceutics | PK/PD Modeling of the PDE7 Inhibitor¡ªGRMS-55 in a Mouse Model of Autoimmune Hepatitis. Pharmaceutics 2021, 13, 597 [33919375] |
| Melatonin-mediated MT2 attenuates colitis induced by dextran sodium sulfate via PI3K/AKT/Nrf2/SIRT1/ROR¦Á/NF-¦ÊB signaling pathways [34162146] | |
| How Does the Th1/Th2 Polarization in Milk Change According to the Types of Bacteria that Cause Subclinical Mastitis in Cows? [] | |
| Amplified Antitumor Efficacy By A Targeted Drugs Retention and Chemosensitization Strategies-Based ¡°Combo¡± Nanoagent Together With PD-L1 Blockade In?¡ [] | |
| World J Tradit Chin Med | Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction via Th2 cytokine enhancement [] |
| Cancer Lett | Photodynamic therapy induces human esophageal carcinoma cell pyroptosis by targeting the PKM2/caspase-8/caspase-3/GSDME axis [34256094] |
| J Pharm Pharmacol | Alamandine protects against renal ischaemia–reperfusion injury in rats via inhibiting oxidative stress [34244746] |
| J Nanobiotechnology | Amplified antitumor efficacy by a targeted drug retention and chemosensitization strategy-based “combo” nanoagent together with PD-L1 blockade in … [34225744] |
| Neoplasia | PDIA6 promotes pancreatic cancer progression and immune escape through CSN5-mediated deubiquitination of β-catenin and PD-L1 [34325342] |
| Sci Rep | Talaromyces marneffei and nontuberculous mycobacteria co-infection in HIV-negative patients [34376749] |
| Am J Transl Res | Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma [34377237] |
| J Immunol Res | Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus [34395633] |
| Sci Rep | Novel interferon-sensitive genes unveiled by correlation-driven gene selection and systems biology [34508139] |
| Transl Psychiatry | Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive … [34802039] |
| Infect Drug Resist | Retrospective Analysis of 10 Cases of Disseminated Nontuberculous Mycobacterial Disease with Osteolytic Lesions [34785914] |
| Environmental Research | In-vitro oxidative potential and inflammatory response of ambient PM2. 5 in a rural region of Northwest China: Association with chemical compositions and … [34863982] |
| Eur J Pharm Sci | Preclinical study of methotrexate-based hydrogels versus surfactant based liquid crystal systems on psoriasis treatment [34314841] |
| J Inflamm Res | Clenbuterol, a Selective β2-Adrenergic Receptor Agonist, Inhibits or Limits Post-Stroke Pneumonia, but Increases Infarct Volume in MCAO Mice [Pubmed:35058704] |
| Poult Sci | Salmonella phage CKT1 significantly relieves the body weight loss of chicks by normalizing the abnormal intestinal microbiome caused by hypervirulent … [Pubmed:35063807] |
| ACS Appl Mater Interfaces | Combinational Chemoimmunotherapy for Breast Cancer by Codelivery of Doxorubicin and PD-L1 siRNA Using a PAMAM-Incorporated Liposomal Nanoplatform [Pubmed:35138103] |
| Theranostics | Combating multidrug resistance and metastasis of breast cancer by endoplasmic reticulum stress and cell-nucleus penetration enhanced … [Pubmed:35401832] |
| Inflammopharmacology | Silymarin constrains diacetyl-prompted oxidative stress and neuroinflammation in rats: involvements of Dyn/GDNF and MAPK signaling pathway [Pubmed:35366745] |
| Journal of Nanobiotechnology | Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM [Pubmed:35351131] |
| Infection and Drug Resistance | Sweet's Syndrome Accompanied by Coinfection with Multiple Pathogens and Disseminated Mycobacterium phlei Infection Presenting with Osteolytic … [Pubmed:35592103] |
| Sci Rep | Clinical findings and predictive factors for positive anti-interferon-γ autoantibodies in patients suffering from a non-tuberculosis mycobacteria or Talaromyces … [Pubmed:35641599] |
| Biomedicines | Deletion of Toxoplasma Rhoptry Protein 38 (PruΔrop38) as a Vaccine Candidate for Toxoplasmosis in a Murine Model [Pubmed:35740356] |
| Viruses | Synergistic Pathogenicity by Coinfection and Sequential Infection with NADC30-like PRRSV and PCV2 in Post-Weaned Pigs [Pubmed:35215787] |
| Antioxidants | Blue Light Alters the Composition of the Jejunal Microbiota and Promotes the Development of the Small Intestine by Reducing Oxidative Stress [Pubmed:35204158] |
| Oxidative Medicine and Cellular Longevity | The Role of Aeromonas-Goblet Cell Interactions in Melatonin-Mediated Improvements in Sleep Deprivation-Induced Colitis [Pubmed:35355860] |
| Journal of Integrative Medicine | Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats [Pubmed:35643766] |
| researchsquare | MSC-EVs: A Promising Modulator for Immune Response in Systemic Lupus Erythematosus [] |
| Mljekarstvo | Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows [] |
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