血清淀粉样蛋白A(SAA)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Serum Amyloid A (SAA) ,etc. by FLIA (Flow Luminescence Immunoassay)
SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein
（注：单次混测多因子不超过8个指标 ）
- 编号LMA885Ga
- 物种Chicken (Gallus，鸡) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.1-100ng/mL
- 灵敏度最小可检测剂量小于等于0.033 ng/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
- 价格 ¥ 2562 ¥ 2660 ¥ 2808 ¥ 3005 ¥ 3202 ¥ 3497 ¥ 3941 ¥ 4926 添加到价格计算器 计算器
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测血清淀粉样蛋白A(SAA)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的血清淀粉样蛋白A(SAA)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 90-97 | 94 |
| EDTA plasma(n=5) | 95-105 | 98 |
| heparin plasma(n=5) | 87-101 | 96 |
| sodium citrate plasma(n=5) | 85-92 | 88 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的血清淀粉样蛋白A(SAA)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血清淀粉样蛋白A(SAA)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-93% | 86-95% | 94-105% | 95-103% |
| EDTA plasma(n=5) | 80-95% | 89-96% | 94-103% | 81-105% |
| heparin plasma(n=5) | 82-97% | 78-96% | 80-104% | 88-96% |
| sodium citrate plasma(n=5) | 79-104% | 84-105% | 90-98% | 85-97% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将血清淀粉样蛋白A(SAA)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的血清淀粉样蛋白A(SAA)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的血清淀粉样蛋白A(SAA)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的血清淀粉样蛋白A(SAA)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Chicken (Gallus，鸡) | 应用(仅供研究使用，不用于临床诊断！) |
| SEA885Ga | 血清淀粉样蛋白A(SAA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA885Ga | 血清淀粉样蛋白A(SAA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Nitric Oxide. | Effect of feeding a high fat diet on hydrogen sulfide (H 2 S) metabolism in the mouse [Pubmed:24637018] |
| Open Biochem J. | Acute Phase Response: Implication in ST-segment Elevation Myocardial Infarction [Pubmed:24894970] |
| Iran J Pharm Res. | Comparison of the Effects of Enoxaparin and Heparin on Inflammatory Biomarkers in Patients with ST-segment Elevated Myocardial Infarction: A prospective Open Label Pilot Clinical Trial [Pubmed:25237354] |
| US National Library of Medicine National Institutes of Health | No association between vitamin D levels and inflammation markers in patients with acute coronary syndrome [Pubmed:25615956] |
| BMC Complementary and Alternative Medicine | Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study [Pubmed:25652121] |
| BOSCA | EFFECT OF UTERINE IMMUNOMODULATION ON SERUM AMYLOID-A CONCENTRATION AND CONCEPTION RATE IN CYCLIC NON BREEDING COWS [Tcrjournals:Source] |
| Life Sciences | Impaired adipogenesis in adipose tissue associated with hepatic lipid deposition induced by chronic inflammation in mice with chew diet [PubMed: 26188592] |
| Endocrine | Relation of thyroid hormone abnormalities with subclinical inflammatory activity in patients with type 1 and type 2 diabetes mellitus [PubMed: 26049370] |
| J Assist Reprod Genet. | The effect of serum and follicular fluid amyloid-associated protein levels on in vitro fertilization outcome in patients with polycystic ovary syndrome [PubMed: 26463878] |
| International Journal of Clinical and Experimental Medicine | Circulating serum amyloid A: a potential biomarker for infection after aneurysmal subarachnoid hemorrhage [files:ijcem0021201] |
| PLoS One. | An Anti-β-Amyloid Vaccine for Treating Cognitive Deficits in a Mouse Model of Down Syndrome [Pubmed:27023444] |
| United States Patent Application | PATHWAY SPECIFIC MARKERS FOR DIAGNOSING IRRITABLE BOWEL SYNDROME [y2016:0139148.html] |
| Veterinary World | Acute phase response in lame crossbred dairy cattle [pubmed:27956769] |
| Nature Communications | A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense [Pubmed:29410422] |
| Journal of Animal Sciences | Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rations [Pubmed:30060086] |
| Journal of Animal Science | Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood … [Pubmed: 30184125] |
| Journal of Dairy Science | Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cows [Pubmed: 17430930] |
| European Review for Medical and Pharmacological Sciences | The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia. [Pubmed: 31298341] |
| Research in Veterinary Science | Evaluation of infrared thermography compared to rectal temperature to identify illness in early postpartum dairy cows [] |
| Journal of Animal Science | Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers [Pubmed: 31410465] |
| PLoS One | Effects of vaccination on acute-phase protein response in broiler chicken [Pubmed: 32191767] |
| VETERINARY PATHOLOGY | AA Amyloid Deposition in the Central and Peripheral Nervous Systems in Flamingos [Pubmed: 32677873] |
| Comp Immunol Microbiol Infect Dis | Serum amyloid A as an marker of cow֨ s mastitis caused by Streptococcus sp. [Pubmed: 32505957] |
| Indian Journal of Animal Sciences | Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy … [] |
| РОЛЬ СЫВОРОТОЧНОГО АМИЛОИДА А И С-РЕАКТИВНОГО БЕЛКА В ПРОГНОЗИРОВАНИИ ПОСЛЕОПЕРАЦИОННЫХ ОСЛОЖНЕНИЙ И … [] | |
| JOURNAL OF ANIMAL SCIENCE | Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient?¡ [33474564] |
| Animals | Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid [34070499] |
| peerj | Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats [33510972] |
| Arquivo Brasileiro de Medicina Veterin¨¢ria e Zootecnia | CCS e amiloide A do leite de quartos mam¨¢rios tratados na secagem com plasma rico em plaquetas aut¨®logo, associado ou n?o a antibi¨®tico [] |
| Probiotics Antimicrob Proteins | The Effect of Feed Supplementation with EM Bokashi® Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators … [34596883] |
| Vet Anim Sci | Effects of dietary turmeric (Curcuma longa) on innate and acquired immune responses in broiler chicken [34765801] |
| Brain Sci | Mahanimbine Improved Aging-Related Memory Deficits in Mice through Enhanced Cholinergic Transmission and Suppressed Oxidative Stress, Amyloid Levels, and … [Pubmed:35053756] |
| J Clin Med | A Three-Protein Panel to Support the Diagnosis of Sepsis in Children [Pubmed:35329889] |
| Diabetes Metabolic Syndrome and Obesity-Targets and Therapy | Serum and salivary adipokines in type 2 diabetes–Results of a pilot study in India [Pubmed:35717896] |
| Clinical and Experimental Immunology | Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patients [Pubmed:35348620] |
| İneklerde Subklinik Mastitis Tanısında Akut Faz Proteinlerinden Amiloid A ve C-Reaktif Protein Düzeylerinin Değerlendirilmesi [] |