组织因子(TF)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Tissue Factor (TF) ,etc. by FLIA (Flow Luminescence Immunoassay)
CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III
（注：单次混测多因子不超过8个指标 ）
- 编号LMA524Rb
- 物种Oryctolagus cuniculus (Rabbit，兔) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.39-400pg/mL
- 灵敏度最小可检测剂量小于等于0.13 pg/mL.
- 样本类型plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测组织因子(TF)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的组织因子(TF)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| EDTA plasma(n=5) | 96-105 | 99 |
| heparin plasma(n=5) | 78-98 | 89 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的组织因子(TF)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中组织因子(TF)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| EDTA plasma(n=5) | 80-98% | 96-103% | 88-96% | 79-88% |
| heparin plasma(n=5) | 80-93% | 78-88% | 88-103% | 91-99% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将组织因子(TF)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的组织因子(TF)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的组织因子(TF)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的组织因子(TF)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Oryctolagus cuniculus (Rabbit，兔) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA524Rb02 | 组织因子(TF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA524Rb01 | 组织因子(TF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA524Rb52 | 组织因子(TF)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA524Rb51 | 组织因子(TF)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA524Rb01 | 组织因子(TF)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA524Rb71 | 组织因子(TF)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA524Rb21 | 组织因子(TF)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA524Rb | 组织因子(TF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA524Rb | 组织因子(TF)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSA524Rb01 | 组织因子(TF)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA524Rb01 | 组织因子(TF)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
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| Canadian Journal of Physiology and Pharmacology | Increased plasma level of asymmetric dimethylarginine in hypertensive rats facilitates platelet aggregation: role of plasma tissue factor [Nrcresearchpress: y10-115] |
| International immunopharmacology | Ruscogenin attenuates monocrotaline-induced pulmonary hypertension in rats [Pubmed: 23538027] |
| BioMed research international | Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192] |
| The Journal of Biological Chemistry | Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy [Pubmed:25505239] |
| Hippokratia | Thrombosis in Hemodialysis Patients; Their Association with Tissue Factor and Tissue Factor Pathway Inhibitor [PubMed: 20011089] |
| shock | Noble-Collip Drum Trauma Induces Disseminated Intravascular Coagulation But Not Acute Coagulopathy of Trauma-Shock [PubMed: 25423126] |
| Perfusion | Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing [PubMed: 26243277] |
| Molecular Nutrition & Food Research | Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors [PubMed: 25546502] |
| Food Funct | The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice [Pubmed:26757699] |
| Blood Cells, Inflammation and Infection | Nebulized Heparin Attenuates Pulmonary Coagulopathy and Inflammation through Alveolar Macrophages in a Rat Model of Acute Lung Injury [pubmed:29202212] |
| Thrombosis andHaemostasis | Nebulized anti-coagulants as a therapy for acute lung injury and acute respiratory distress syndrome [Pubmed: 29202212] |
| Journal of Thrombosis and Haemostasis | Effects of nebulized antithrombin and heparin on inflammatory and coagulation alterations in an acute lung injury model in rats [Pubmed: 31755229] |
| Mediators Inflamm | Amelioration of Coagulation Disorders and Inflammation by Hydrogen-Rich Solution Reduces Intestinal Ischemia/Reperfusion Injury in Rats through NF-κB … [Pubmed: 32587471] |
| International Journal of Clinical Practice | Effect of Glucocorticoid Administration in Intravenous Pulses on Selected Parameters of the Coagulation System [Pubmed:35685509] |