表面活性物质关联蛋白B(SP-B)重组蛋白
Recombinant Surfactant Protein B (SP-B)
SFTPB; PSPB; SFTB3; SFTP3; SPB; Surfactant Associated Protein B; Pulmonary Surfactant Protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein
- 编号RPB622Ra01
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位分泌, 细胞外基质
- 预测分子量28.3kDa
- 实际分子量26kDa(差异分析请参阅说明书)
- 片段与标签Arg111~His329 with N-terminal His Tag
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 97%
- 等电点8.3
-
应用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,请参见活性蛋白。 - 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1075 ¥ 2688 ¥ 5376 ¥ 16128 ¥ 40320
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPB622Ra01 | 表面活性物质关联蛋白B(SP-B)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB622Ra01 | 表面活性物质关联蛋白B(SP-B)多克隆抗体 | WB; IHC; ICC; IP. |
MAB622Ra21 | 表面活性物质关联蛋白B(SP-B)单克隆抗体 | WB; IHC; ICC; IP. |
SEB622Ra | 表面活性物质关联蛋白B(SP-B)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB622Ra | 表面活性物质关联蛋白B(SP-B)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB622Ra01 | 表面活性物质关联蛋白B(SP-B)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Respiratory Physiology & Neurobiology | Kinetics of plasma SPB and RAGE during mechanical ventilation in patients undergoing major vascular surgery [PubMed: 21736957] |
American Journal of Rhinology & Allergy | Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps [PubMed: 23406594] |
19 | Surfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass: a pilot study [PubMed: 20650982] |
Plos one | Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins [PubMed: PMC3551896] |
Plos one | The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS [PubMed: PMC3787032] |
Cancer Cell. | Lung Cancer Signatures in Plasma Based on Proteome Profiling of Mouse Tumor Models [PubMed: PMC3406925] |
Respir Physiol Neurobiol | Acute high-altitude exposure reduces lung diffusion: Data from the HIGHCARE Alps project [Pubmed: 23619193] |
PLOS ONE | Surfactant-Derived Proteins as Markers of Alveolar Membrane Damage in Heart Failure [Pubmed:25514679] |
Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge [Opus4:Source] | |
Int J Cardiol | Plasma immature form of surfactant protein type B correlates with prognosis in patients with chronic heart failure. A pilot single-center prospective study [PubMed: 26310985] |
Plos One | Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE) [Pubmed:27337142] |
PLoS One | The Cerebral Surfactant System and Its Alteration in HydrocephalicConditions. [pubmed:27656877] |
Frontiers in Aging Neuroscience | Correlations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid. [pubmed:28101052] |
Frontiers in Aging Neuroscience | Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid [PMC5209370] |
Respiratory Physiology & Neurobiology | Diving and pulmonary physiology: surfactant binding protein, lung fluid and cardiopulmonary test changes in professional divers [pubmed:28467885] |
Cellular Physiology and Biochemistry | Surfactant Protein B Suppresses Lung Cancer Progression by Inhibiting Secretory Phospholipase A2 Activity and Arachidonic Acid Production [pubmed:28743125] |
American Journal of Physiology. Lung Cellular and Molecular Physiology | Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency. [pubmed:29167126] |
Respiratory research | Effects and molecular mechanisms of intrauterine infection/inflammation on lung development [Pubmed:29747649] |
Respiratory Physiology & Neurobiology | Surfactant proteins changes after acute hemodynamic improvement in patients with advanced chronic heart failure treated with Levosimendan [Pubmed:29548887] |
Lung | Serum Surfactant Protein Levels in Patients Admitted to the Hospital with Acute COPD Exacerbation [Pubmed:29445934] |
American Journal of Physiology Lung Cellular andMolecular Physiology | Generation of an alveolar epithelial type II cell line from induced pluripotent stem cells [Pubmed: 30211653] |
Respiratory Research | Culture of human alveolar epithelial type II cells by sprouting [Pubmed: 30340591] |
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