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甲状旁腺激素相关蛋白(PTHrP)活性蛋白

Active Parathyroid Hormone Related Protein (PTHrP)

PTHLH; HHM; PTHR; PLP; Osteostatin; Parathyroid Hormone Like Hormone

  • 甲状旁腺激素相关蛋白(PTHrP)活性蛋白 产品包装(模拟)
  • 甲状旁腺激素相关蛋白(PTHrP)活性蛋白 产品包装(模拟)
  • 甲状旁腺激素相关蛋白(PTHrP)活性蛋白 Gene sequencing
  • APA819Mu01.jpg SDS-PAGE图
  • 甲状旁腺激素相关蛋白(PTHrP)活性蛋白 Figure. Western Blot; Sample: Recombinant PTHrP, Mouse.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

PTHrP (Parathyroid Hormone Related Protein ) is a neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. A binding ELISA assay was conducted to detect the association of PTHrP with PTHR1. Briefly, PTHrP were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL PTHrP were then transferred to PTHR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PTHrP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of PTHrP and PTHR1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPA819Mu01 甲状旁腺激素相关蛋白(PTHrP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA819Mu02 甲状旁腺激素相关蛋白(PTHrP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA819Mu01 甲状旁腺激素相关蛋白(PTHrP)活性蛋白 Cell culture; Activity Assays.
CPA819Mu21 甲状旁腺激素相关蛋白(PTHrP)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA819Mu22 甲状旁腺激素相关蛋白(PTHrP)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA819Mu31 甲状旁腺激素相关蛋白(PTHrP)钥孔血蓝蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA819Mu01 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 WB; IHC; ICC; IP.
PAA819Mu02 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 WB; IHC; ICC; IP.
PAA819Mu08 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 WB; IHC; ICC; IP.
SEA819Mu 甲状旁腺激素相关蛋白(PTHrP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA819Mu 甲状旁腺激素相关蛋白(PTHrP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA819Mu 甲状旁腺激素相关蛋白(PTHrP)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA819Mu11 甲状旁腺激素相关蛋白(PTHrP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Clinical and Experimental Metastasis Characterization of a new renal cell carcinoma bone metastasis mouse model [PubMed: 20443133]
Stem Cells and Development Temporal activation of β-catenin signaling in the chondrogenic process of mesenchymal stem cells affects the phenotype of the cartilage generated. [PubMed: 22133004]
International journal of biological sciences PTHrP Expression in Human MDA-MB-231 Breast Cancer Cells Is Critical for Tumor Growth and Survival and Osteoblast Inhibition [PubMed: PMC3753447]
Investigational New Drugs Tetrahydrofurofuran-type lignans inhibit breast cancer-mediated bone destruction by blocking the vicious cycle between cancer cells, osteoblasts and osteoclasts [Pubmed: 23673814]
Toxicology and Applied Pharmacology Betulinic acid, a bioactive pentacyclic triterpenoid, inhibits skeletal-related events induced by breast cancer bone metastases and treatment [Pubmed:24463094]
Arch Gynecol Obstet. Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control study [Pubmed:24943060]
PLoS One 15-Deoxy-Δ12, 14-Prostaglandin J2 Inhibits Osteolytic Breast Cancer Bone Metastasis and Estrogen Deficiency-Induced Bone Loss [PubMed: 25859665]
Endocrinology A role for the calcitonin receptor to limit bone loss during lactation in female mice by inhibiting osteocytic osteolysis [PubMed: 26135836]
Tumour Biol Osteopontin expression in co-cultures of human squamous cell carcinoma-derived cells and osteoblastic cells and its effects on the neoplastic cell phenotype and osteoclastic activation. [Pubmed:27312487]
35 Hedgehog signaling in bone regulates whole-body energy metabolism through a bone–adipose endocrine relay mediated by PTHrP and adiponectin [pubmed:27740628]
Bone Lactation induces increases in the RANK/RANKL/OPG system in maxillary bone [Pubmed:29382610]
International Journal of Molecular Sciences A Diet Rich in Fish Oil and Leucine Ameliorates Hypercalcemia in Tumour-Induced Cachectic Mice [Pubmed: 31600911]
Journal of Orthopaedic Surgery and Research Identification of a potential gene target for osteoarthritis based on bioinformatics analyses [Pubmed: 32571421]
International Immunopharmacology Parathyroid hormone related protein (PTHrP)-mediated hypercalcemia in malignancy associated with anti-PD-1 immune checkpoint inhibitor treatment and related … []
Паратиреоидный гормон-родственный протеин как предиктор развития мочекаменной болезни []
Cell Death Dis Extracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer ¡­ [33510128]
Sci Rep Effects of whole-body vibration training in a cachectic C26 mouse model [34732809]
Acta Physiologica The acute adaptation of renal phosphate transporters in the murine kidney to oral phosphate intake requires multiple signals [Pubmed:35334154]
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