太阳集团tyc5997_平台(china)官方网站

甲状旁腺激素相关蛋白(PTHrP)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Parathyroid Hormone Related Protein (PTHrP) ,etc. by FLIA (Flow Luminescence Immunoassay)

PTHLH; HHM; PTHR; PLP; Osteostatin; Parathyroid Hormone Like Hormone

（注：单次混测多因子不超过8个指标 ）

  • 甲状旁腺激素相关蛋白(PTHrP)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 甲状旁腺激素相关蛋白(PTHrP)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测甲状旁腺激素相关蛋白(PTHrP)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的甲状旁腺激素相关蛋白(PTHrP)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 80-97 85
EDTA plasma(n=5) 84-93 89
heparin plasma(n=5) 95-102 99

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的甲状旁腺激素相关蛋白(PTHrP)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中甲状旁腺激素相关蛋白(PTHrP)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 79-97% 80-98% 90-104% 78-97%
EDTA plasma(n=5) 96-105% 78-103% 86-101% 87-96%
heparin plasma(n=5) 98-105% 94-105% 82-98% 87-96%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将甲状旁腺激素相关蛋白(PTHrP)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的甲状旁腺激素相关蛋白(PTHrP)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的甲状旁腺激素相关蛋白(PTHrP)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的甲状旁腺激素相关蛋白(PTHrP)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPA819Mu01 甲状旁腺激素相关蛋白(PTHrP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA819Mu02 甲状旁腺激素相关蛋白(PTHrP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA819Mu01 甲状旁腺激素相关蛋白(PTHrP)活性蛋白 Cell culture; Activity Assays.
CPA819Mu21 甲状旁腺激素相关蛋白(PTHrP)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA819Mu22 甲状旁腺激素相关蛋白(PTHrP)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA819Mu31 甲状旁腺激素相关蛋白(PTHrP)钥孔血蓝蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA819Mu01 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 WB; IHC; ICC; IP.
PAA819Mu02 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 WB; IHC; ICC; IP.
PAA819Mu08 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 WB; IHC; ICC; IP.
SEA819Mu 甲状旁腺激素相关蛋白(PTHrP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA819Mu 甲状旁腺激素相关蛋白(PTHrP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA819Mu 甲状旁腺激素相关蛋白(PTHrP)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA819Mu11 甲状旁腺激素相关蛋白(PTHrP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Clinical and Experimental Metastasis Characterization of a new renal cell carcinoma bone metastasis mouse model [PubMed: 20443133]
Stem Cells and Development Temporal activation of β-catenin signaling in the chondrogenic process of mesenchymal stem cells affects the phenotype of the cartilage generated. [PubMed: 22133004]
International journal of biological sciences PTHrP Expression in Human MDA-MB-231 Breast Cancer Cells Is Critical for Tumor Growth and Survival and Osteoblast Inhibition [PubMed: PMC3753447]
Investigational New Drugs Tetrahydrofurofuran-type lignans inhibit breast cancer-mediated bone destruction by blocking the vicious cycle between cancer cells, osteoblasts and osteoclasts [Pubmed: 23673814]
Toxicology and Applied Pharmacology Betulinic acid, a bioactive pentacyclic triterpenoid, inhibits skeletal-related events induced by breast cancer bone metastases and treatment [Pubmed:24463094]
Arch Gynecol Obstet. Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control study [Pubmed:24943060]
PLoS One 15-Deoxy-Δ12, 14-Prostaglandin J2 Inhibits Osteolytic Breast Cancer Bone Metastasis and Estrogen Deficiency-Induced Bone Loss [PubMed: 25859665]
Endocrinology A role for the calcitonin receptor to limit bone loss during lactation in female mice by inhibiting osteocytic osteolysis [PubMed: 26135836]
Tumour Biol Osteopontin expression in co-cultures of human squamous cell carcinoma-derived cells and osteoblastic cells and its effects on the neoplastic cell phenotype and osteoclastic activation. [Pubmed:27312487]
35 Hedgehog signaling in bone regulates whole-body energy metabolism through a bone–adipose endocrine relay mediated by PTHrP and adiponectin [pubmed:27740628]
Bone Lactation induces increases in the RANK/RANKL/OPG system in maxillary bone [Pubmed:29382610]
International Journal of Molecular Sciences A Diet Rich in Fish Oil and Leucine Ameliorates Hypercalcemia in Tumour-Induced Cachectic Mice [Pubmed: 31600911]
Journal of Orthopaedic Surgery and Research Identification of a potential gene target for osteoarthritis based on bioinformatics analyses [Pubmed: 32571421]
International Immunopharmacology Parathyroid hormone related protein (PTHrP)-mediated hypercalcemia in malignancy associated with anti-PD-1 immune checkpoint inhibitor treatment and related … []
Паратиреоидный гормон-родственный протеин как предиктор развития мочекаменной болезни []
Cell Death Dis Extracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer ¡­ [33510128]
Sci Rep Effects of whole-body vibration training in a cachectic C26 mouse model [34732809]
Acta Physiologica The acute adaptation of renal phosphate transporters in the murine kidney to oral phosphate intake requires multiple signals [Pubmed:35334154]
留言咨询
Baidu
sogou