甲状旁腺激素相关蛋白(PTHrP)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Parathyroid Hormone Related Protein (PTHrP)
PTHLH; HHM; PTHR; PLP; Osteostatin; Parathyroid Hormone Like Hormone
- 编号CEA819Mu
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围12.35-1,000pg/mL
- 灵敏度最小可检测剂量小于等于5.35pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2722 ¥ 3888 ¥ 17496 ¥ 33048 ¥ 272160
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测甲状旁腺激素相关蛋白(PTHrP)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的甲状旁腺激素相关蛋白(PTHrP)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 86-102 | 96 |
| EDTA plasma(n=5) | 96-103 | 101 |
| heparin plasma(n=5) | 80-92 | 89 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的甲状旁腺激素相关蛋白(PTHrP)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中甲状旁腺激素相关蛋白(PTHrP)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-103% | 79-93% | 78-98% | 80-99% |
| EDTA plasma(n=5) | 89-96% | 83-97% | 78-94% | 78-94% |
| heparin plasma(n=5) | 78-103% | 96-105% | 87-95% | 79-96% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将甲状旁腺激素相关蛋白(PTHrP)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA819Mu01 | 甲状旁腺激素相关蛋白(PTHrP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA819Mu02 | 甲状旁腺激素相关蛋白(PTHrP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA819Mu01 | 甲状旁腺激素相关蛋白(PTHrP)活性蛋白 | Cell culture; Activity Assays. |
| CPA819Mu21 | 甲状旁腺激素相关蛋白(PTHrP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA819Mu22 | 甲状旁腺激素相关蛋白(PTHrP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA819Mu31 | 甲状旁腺激素相关蛋白(PTHrP)钥孔血蓝蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA819Mu01 | 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA819Mu02 | 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA819Mu08 | 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 | WB; IHC; ICC; IP. |
| SEA819Mu | 甲状旁腺激素相关蛋白(PTHrP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA819Mu | 甲状旁腺激素相关蛋白(PTHrP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA819Mu | 甲状旁腺激素相关蛋白(PTHrP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA819Mu11 | 甲状旁腺激素相关蛋白(PTHrP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
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| International journal of biological sciences | PTHrP Expression in Human MDA-MB-231 Breast Cancer Cells Is Critical for Tumor Growth and Survival and Osteoblast Inhibition [PubMed: PMC3753447] |
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| Tumour Biol | Osteopontin expression in co-cultures of human squamous cell carcinoma-derived cells and osteoblastic cells and its effects on the neoplastic cell phenotype and osteoclastic activation. [Pubmed:27312487] |
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| Bone | Lactation induces increases in the RANK/RANKL/OPG system in maxillary bone [Pubmed:29382610] |
| International Journal of Molecular Sciences | A Diet Rich in Fish Oil and Leucine Ameliorates Hypercalcemia in Tumour-Induced Cachectic Mice [Pubmed: 31600911] |
| Journal of Orthopaedic Surgery and Research | Identification of a potential gene target for osteoarthritis based on bioinformatics analyses [Pubmed: 32571421] |
| International Immunopharmacology | Parathyroid hormone related protein (PTHrP)-mediated hypercalcemia in malignancy associated with anti-PD-1 immune checkpoint inhibitor treatment and related … [] |
| Паратиреоидный гормон-родственный протеин как предиктор развития мочекаменной болезни [] | |
| Cell Death Dis | Extracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer ¡ [33510128] |
| Sci Rep | Effects of whole-body vibration training in a cachectic C26 mouse model [34732809] |
| Acta Physiologica | The acute adaptation of renal phosphate transporters in the murine kidney to oral phosphate intake requires multiple signals [Pubmed:35334154] |
