不对称二甲基精氨酸(ADMA)多克隆抗体(生物素标记)
Biotin-Linked Polyclonal Antibody to Asymmetrical Dimethylarginine (ADMA)
N,N-Dimethylarginine; NG,NG-Dimethylarginine dihydrochloride; Asymmetric Dimethylarginine
- 编号LAB301Ge71
- 物种Pan-species (General,通用) 相同的名称,不同的物种。
- 来源抗体标记
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物生物素
- 原抗体 PAB301Ge01-不对称二甲基精氨酸(ADMA)多克隆抗体
- 缓冲液成份磷酸盐缓冲液(pH7.4,含有0.02% NaN3和50%甘油)
- 性状液体
- 浓度500µg/mL
- 且适物种Pan-species (General,通用)
- 应用WB; IHC; ICC.
如果抗体需用于流式细胞术,请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 787 ¥ 1835 ¥ 2622 ¥ 6555 ¥ 26220
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
该抗体是针对ADMA的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别ADMA。
用法
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
赠品
相关产品
编号 | 适用物种:Pan-species (General,通用) | 应用(仅供研究使用,不用于临床诊断!) |
CPB301Ge11 | 不对称二甲基精氨酸(ADMA)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPB301Ge21 | 不对称二甲基精氨酸(ADMA)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
PAB301Ge01 | 不对称二甲基精氨酸(ADMA)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
LAB301Ge71 | 不对称二甲基精氨酸(ADMA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
CEB301Ge | 不对称二甲基精氨酸(ADMA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB301Ge | 不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB301Ge11 | 不对称二甲基精氨酸(ADMA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
PLoS One. | Interactions among Vascular-Tone Modulators Contribute to High Altitude Pulmonary Edema and Augmented Vasoreactivity in Highlanders [PubMed: PMC3439466] |
Cytokine | Effect of CCL5 on dimethylarginine dimethylaminohydrolase-1 production in vascular smooth muscle cells from spontaneously hypertensive rats. [Pubmed: 23834755] |
Neurol Res. | Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats. [Pubmed:25475507] |
Int J Clin Exp Med. | Correlation research on ADMA plasma levels and left ventricular function of peritoneal dialysis patients [Pubmed:25550968] |
Clin Respir J | Asymmetric dimethylarginine contributes to airway nitric oxide deficiency in patients with COPD [PubMed: 26076870] |
Cytokine | Dimethylarginine dimethylaminohydrolase-1 mediates inhibitory effect of interleukin-10 on angiotensin II-induced hypertensive effects in vascular smooth muscle cells of spontaneously hypertensive rats [PubMed: 26375520] |
Neurological Research | Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats [PubMed: 25475507] |
Scientific Reports | Dimethylarginine Dimethylaminohydrolase 2 (DDAH 2) Gene Polymorphism, Asymmetric Dimethylarginine (ADMA) Concentrations, and Risk of Coronary Artery Disease: A Case-Control Study [pubmed:27677852] |
clinica chimica acta | Increased serum concentrations of asymmetric dimethylarginine (ADMA) in patients with early-onset coronary artery disease. [pubmed:27884754] |
The Journal of Physiology | Asymmetric dimethylarginine (ADMA) elevation and arginase up-regulation contribute toendothelial dysfunction related to insulin resistance in rats and morbidly obese humans. [pubmed:26840628] |
Angiogenesis | Dimethylarginine dimethylaminohydrolase-1 (DDAH1) is frequently upregulated in prostate cancer, and its overexpression conveys tumor growth and angiogenesis by metabolizing asymmetric dimethylarginine (ADMA). [pubmed:29150732] |
Scientific Reports | A Novel Role of Irbesartan in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats: Targeting DDAH/ADMA and EGFR/ERK Signaling [Pubmed:29523851] |
Mechanisms of Ageing and Development | Multivessel analysis of progressive vascular aging in the rat: Asynchronous vulnerability among vascular territories [Pubmed:29605639] |
Nutrition, Metabolism and Cardiovascular Diseases | Arginine bioavailability and endothelin-1 system in the regulation of vascular function of umbilical vein endothelial cells from intrauterine growth restricted newborns [Doi: 10.1016/j.numecd.2018.09.002] |
Biochemical and Biophysical Research Communications | Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic rats [Pubmed: 30454888] |
Brazilian Journal of Pharmaceutical Science | Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients [] |
ACS Combinatorial Science | Novel cellularly active inhibitor regresses DDAH1 induced prostate tumor growth by restraining tumor angiogenesis through targeting DDAH1/ADMA/NOS pathway [Pubmed: 30673277] |
International Journal of the Cardiovascular Academy | Nebivolol prevents the increase of asymmetric dimethylarginine and oxidants in hyperhomocysteinemic rats [] |
Oxid Med Cell Longev | The Role of Reactive Oxygen Species and Nitric Oxide in the Inhibition of Trichophyton rubrum Growth by HaCaT Cells [Pubmed: 32104540] |
Molecular Medicine Reports | Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in … [Pubmed: 31746378] |
International Journal of Nephrology | Effect of a Supervised Peridialytic Exercise Program on Serum Asymmetric Dimethylarginine in Maintenance Hemodialysis Patients [Pubmed: 33163233] |
Journal of Clinical Medicine | The Usefulness of Serum Biomarkers in the Early Stages of Diabetic Retinopathy: Results of the EUROCONDOR Clinical Trial [Pubmed: 32344735] |
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub | Levels of endothelial substances in patients with newly identified hypertension compared with healthy controls [33463628] |
WIENER KLINISCHE WOCHENSCHRIFT | Non-functioning adrenal incidentalomas may increase toxic metabolites [34255169] |
pharmaceuticals | Effect of a Low Dose of Carvedilol on Cyclophosphamide-Induced Urinary Toxicity in Rats—A Comparison with Mesna [34959638] |
Int J Biochem Cell Biol | HIF-1α and Nrf2 regulates hypoxia induced overexpression of DDAH1 through promoter activation in prostate cancer [Pubmed:35644470] |
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