吲哚胺2,3-双加氧酶(IDO)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Indoleamine-2,3-Dioxygenase (IDO)
CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase
- 编号KSB547Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.312-20ng/mL
- 可适灵敏度0.131ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7182 ¥ 11970 ¥ 21546 ¥ 41895 ¥ 71820
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对吲哚胺2,3-双加氧酶(IDO)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPB547Hu01 | 吲哚胺2,3-双加氧酶(IDO)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB547Hu01 | 吲哚胺2,3-双加氧酶(IDO)活性蛋白 | Cell culture; Activity Assays. |
PAB547Hu01 | 吲哚胺2,3-双加氧酶(IDO)多克隆抗体 | WB; IHC; ICC; IP. |
LAB547Hu71 | 吲哚胺2,3-双加氧酶(IDO)多克隆抗体(生物素标记) | WB; IHC; ICC. |
LAB547Hu81 | 吲哚胺2,3-双加氧酶(IDO)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
MAB547Hu22 | 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | WB; IHC; ICC; IP. |
MAB547Hu21 | 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | WB; IHC. |
MAB547Hu23 | 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | WB; IHC; ICC; IP. |
FAB547Hu08 | 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | Flow cytometry. |
FAB547Hu06 | 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | Flow cytometry. |
FAB547Hu04 | 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | Flow cytometry. |
FAB547Hu02 | 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | Flow cytometry. |
LAB547Hu72 | 吲哚胺2,3-双加氧酶(IDO)单克隆抗体(生物素标记) | WB; IHC; ICC. |
SEB547Hu | 吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB547Hu | 吲哚胺2,3-双加氧酶(IDO)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
PSB547Hu01 | 吲哚胺2,3-双加氧酶(IDO)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSB547Hu01 | 吲哚胺2,3-双加氧酶(IDO)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Blood | CCL18 differentiates dendritic cells in tolerogenic cells able to prime regulatory T cells in healthy subjects [Pubmed: 21803856] |
Clinical Transplantation | Potential immunosuppressive function of plasma indoleamine 2,3-dioxygenase in patients with aGVHD after allo-HSCT [Wiley: source] |
Indonesian Journal of Cancer Chemoprevention | Secretion of Indoleamine 2, 3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem Cell [Iregway: Source] |
PLoS ONE | CD40 Gene Silencing Reduces the Progression of Experimental Lupus Nephritis Modulating Local Milieu and Systemic Mechanisms [PubMed: PMC3683035] |
Hepatology | Human CD14+ CTLA-4+ regulatory dendritic cells suppress T-cell response by cytotoxic T-lymphocyte antigen-4-dependent IL-10 and indoleamine-2,3-dioxygenase production in hepatocellular carcinoma [Pubmed: 23960017] |
Free Radical Biology and Medicine | Indoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocans [ScienceDirect: S0891584913006345] |
Frontiers in Oncology | Cancer-Associated Fibroblasts from lung tumors maintain their immuno-suppressive abilities after high-dose irradiation [Frontiersin:Source] |
Tissue Engineering Part A | Immunogenicity and escape mechanisms of allogeneic tendon-derived stem cells [Pubmed:24813640] |
Immunobiology. | The expression of dendritic cell subsets in severe chronic rhinosinusitis with nasal polyps is altered [Pubmed:24947894] |
Apoptosis | Chronic hepatitis C virus infection triggers spontaneous differential expression of biosignatures associated with T cell exhaustion and apoptosis signaling in peripheral blood mononucleocytes [Pubmed:25577277] |
Front Oncol | Cancer-Associated Fibroblasts from Lung Tumors Maintain Their Immunosuppressive Abilities after High-Dose Irradiation [PubMed: 26029659] |
Acta Universitatis Tamperensis | Insights into the use of adipose stem cells for clinical cell therapy: Novel culturing conditions and characterization of multipotency and immunogenic properties [Handle: 10024] |
PLoS One | Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2, 3-Dioxygenase [pubmed:27695127] |
Journal of Biomedical Nanotechnology | Exploring Amino Acid-Capped Nanoparticles for Selective Anti-Parasitic Action and Improved Host Biocompatibility [Pubmed:29883557] |
Biochemistry | Mechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells [] |
American Journal of Pathology | Cognitive Decline, Cerebral-Spleen Tryptophan Metabolism, Oxidative Stress, Cytokine Production, and Regulation of the Txnip Gene in 3xTg-AD Mice [Pubmed: 30980800] |
international immunopharmacology | Interaction of the immune-inflammatory and the kynurenine pathways in rats resistant to antidepressant treatment in model of depression [Pubmed: 31176083] |
Stem Cell Reports | Richard Schäfer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Kuçi, 4 [] |
International Journal of?Tryptophan?Research | Altered Indoleamine 2, 3-Dioxygenase Production and Its Association to Inflammatory Cytokines in Peripheral Blood Mononuclear Cells Culture of Type 2 Diabetes?¡ [33343199] |
Stem Cell Reports | Richard Sch?fer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Ku?i, 4 [] |
Transl Psychiatry | Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive … [34802039] |
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