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吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Indoleamine-2,3-Dioxygenase (IDO)

CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase

  • 吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEB547Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测吲哚胺2,3-双加氧酶(IDO),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的吲哚胺2,3-双加氧酶(IDO)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 80-104 97
EDTA plasma(n=5) 79-105 94
heparin plasma(n=5) 96-103 99

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的吲哚胺2,3-双加氧酶(IDO),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中吲哚胺2,3-双加氧酶(IDO)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 82-93% 83-92% 78-99% 89-104%
EDTA plasma(n=5) 85-93% 80-94% 86-104% 79-90%
heparin plasma(n=5) 96-103% 78-93% 84-104% 87-94%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将吲哚胺2,3-双加氧酶(IDO)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的吲哚胺2,3-双加氧酶(IDO)与连接于固相载体上的抗体结合,然后加入生物素化的吲哚胺2,3-双加氧酶(IDO)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的吲哚胺2,3-双加氧酶(IDO)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPB547Hu01 吲哚胺2,3-双加氧酶(IDO)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB547Hu01 吲哚胺2,3-双加氧酶(IDO)活性蛋白 Cell culture; Activity Assays.
PAB547Hu01 吲哚胺2,3-双加氧酶(IDO)多克隆抗体 WB; IHC; ICC; IP.
LAB547Hu71 吲哚胺2,3-双加氧酶(IDO)多克隆抗体(生物素标记) WB; IHC; ICC.
LAB547Hu81 吲哚胺2,3-双加氧酶(IDO)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAB547Hu22 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 WB; IHC; ICC; IP.
MAB547Hu21 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 WB; IHC.
MAB547Hu23 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 WB; IHC; ICC; IP.
FAB547Hu08 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 Flow cytometry.
FAB547Hu06 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 Flow cytometry.
FAB547Hu04 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 Flow cytometry.
FAB547Hu02 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 Flow cytometry.
LAB547Hu72 吲哚胺2,3-双加氧酶(IDO)单克隆抗体(生物素标记) WB; IHC; ICC.
SEB547Hu 吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB547Hu 吲哚胺2,3-双加氧酶(IDO)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
PSB547Hu01 吲哚胺2,3-双加氧酶(IDO)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB547Hu01 吲哚胺2,3-双加氧酶(IDO)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Blood CCL18 differentiates dendritic cells in tolerogenic cells able to prime regulatory T cells in healthy subjects [Pubmed: 21803856]
Clinical Transplantation Potential immunosuppressive function of plasma indoleamine 2,3-dioxygenase in patients with aGVHD after allo-HSCT [Wiley: source]
Indonesian Journal of Cancer Chemoprevention Secretion of Indoleamine 2, 3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem Cell [Iregway: Source]
PLoS ONE CD40 Gene Silencing Reduces the Progression of Experimental Lupus Nephritis Modulating Local Milieu and Systemic Mechanisms [PubMed: PMC3683035]
Hepatology Human CD14+ CTLA-4+ regulatory dendritic cells suppress T-cell response by cytotoxic T-lymphocyte antigen-4-dependent IL-10 and indoleamine-2,3-dioxygenase production in hepatocellular carcinoma [Pubmed: 23960017]
Free Radical Biology and Medicine Indoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocans [ScienceDirect: S0891584913006345]
Frontiers in Oncology Cancer-Associated Fibroblasts from lung tumors maintain their immuno-suppressive abilities after high-dose irradiation [Frontiersin:Source]
Tissue Engineering Part A Immunogenicity and escape mechanisms of allogeneic tendon-derived stem cells [Pubmed:24813640]
Immunobiology. The expression of dendritic cell subsets in severe chronic rhinosinusitis with nasal polyps is altered [Pubmed:24947894]
Apoptosis Chronic hepatitis C virus infection triggers spontaneous differential expression of biosignatures associated with T cell exhaustion and apoptosis signaling in peripheral blood mononucleocytes [Pubmed:25577277]
Front Oncol Cancer-Associated Fibroblasts from Lung Tumors Maintain Their Immunosuppressive Abilities after High-Dose Irradiation [PubMed: 26029659]
Acta Universitatis Tamperensis Insights into the use of adipose stem cells for clinical cell therapy: Novel culturing conditions and characterization of multipotency and immunogenic properties [Handle: 10024]
PLoS One Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2, 3-Dioxygenase [pubmed:27695127]
Journal of Biomedical Nanotechnology Exploring Amino Acid-Capped Nanoparticles for Selective Anti-Parasitic Action and Improved Host Biocompatibility [Pubmed:29883557]
Biochemistry Mechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells []
American Journal of Pathology Cognitive Decline, Cerebral-Spleen Tryptophan Metabolism, Oxidative Stress, Cytokine Production, and Regulation of the Txnip Gene in 3xTg-AD Mice [Pubmed: 30980800]
international immunopharmacology Interaction of the immune-inflammatory and the kynurenine pathways in rats resistant to antidepressant treatment in model of depression [Pubmed: 31176083]
Stem Cell Reports Richard Schäfer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Kuçi, 4 []
International Journal of?Tryptophan?Research Altered Indoleamine 2, 3-Dioxygenase Production and Its Association to Inflammatory Cytokines in Peripheral Blood Mononuclear Cells Culture of Type 2 Diabetes?¡­ [33343199]
Stem Cell Reports Richard Sch?fer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Ku?i, 4 []
Transl Psychiatry Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive … [34802039]
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