吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Indoleamine-2,3-Dioxygenase (IDO)
CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase
- 编号SEB547Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.312-20ng/mL
- 灵敏度最小可检测剂量小于等于0.127ng/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2793 ¥ 3990 ¥ 17955 ¥ 33915 ¥ 279300
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测吲哚胺2,3-双加氧酶(IDO)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的吲哚胺2,3-双加氧酶(IDO)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 80-104 | 97 |
| EDTA plasma(n=5) | 79-105 | 94 |
| heparin plasma(n=5) | 96-103 | 99 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的吲哚胺2,3-双加氧酶(IDO)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中吲哚胺2,3-双加氧酶(IDO)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-93% | 83-92% | 78-99% | 89-104% |
| EDTA plasma(n=5) | 85-93% | 80-94% | 86-104% | 79-90% |
| heparin plasma(n=5) | 96-103% | 78-93% | 84-104% | 87-94% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将吲哚胺2,3-双加氧酶(IDO)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的吲哚胺2,3-双加氧酶(IDO)与连接于固相载体上的抗体结合，然后加入生物素化的吲哚胺2,3-双加氧酶(IDO)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的吲哚胺2,3-双加氧酶(IDO)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB547Hu01 | 吲哚胺2,3-双加氧酶(IDO)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB547Hu01 | 吲哚胺2,3-双加氧酶(IDO)活性蛋白 | Cell culture; Activity Assays. |
| PAB547Hu01 | 吲哚胺2,3-双加氧酶(IDO)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB547Hu71 | 吲哚胺2,3-双加氧酶(IDO)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAB547Hu81 | 吲哚胺2,3-双加氧酶(IDO)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAB547Hu22 | 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB547Hu21 | 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | WB; IHC. |
| MAB547Hu23 | 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | WB; IHC; ICC; IP. |
| FAB547Hu08 | 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | Flow cytometry. |
| FAB547Hu06 | 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | Flow cytometry. |
| FAB547Hu04 | 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | Flow cytometry. |
| FAB547Hu02 | 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | Flow cytometry. |
| LAB547Hu72 | 吲哚胺2,3-双加氧酶(IDO)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEB547Hu | 吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB547Hu | 吲哚胺2,3-双加氧酶(IDO)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSB547Hu01 | 吲哚胺2,3-双加氧酶(IDO)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSB547Hu01 | 吲哚胺2,3-双加氧酶(IDO)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Blood | CCL18 differentiates dendritic cells in tolerogenic cells able to prime regulatory T cells in healthy subjects [Pubmed: 21803856] |
| Clinical Transplantation | Potential immunosuppressive function of plasma indoleamine 2,3-dioxygenase in patients with aGVHD after allo-HSCT [Wiley: source] |
| Indonesian Journal of Cancer Chemoprevention | Secretion of Indoleamine 2, 3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem Cell [Iregway: Source] |
| PLoS ONE | CD40 Gene Silencing Reduces the Progression of Experimental Lupus Nephritis Modulating Local Milieu and Systemic Mechanisms [PubMed: PMC3683035] |
| Hepatology | Human CD14+ CTLA-4+ regulatory dendritic cells suppress T-cell response by cytotoxic T-lymphocyte antigen-4-dependent IL-10 and indoleamine-2,3-dioxygenase production in hepatocellular carcinoma [Pubmed: 23960017] |
| Free Radical Biology and Medicine | Indoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocans [ScienceDirect: S0891584913006345] |
| Frontiers in Oncology | Cancer-Associated Fibroblasts from lung tumors maintain their immuno-suppressive abilities after high-dose irradiation [Frontiersin:Source] |
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| Front Oncol | Cancer-Associated Fibroblasts from Lung Tumors Maintain Their Immunosuppressive Abilities after High-Dose Irradiation [PubMed: 26029659] |
| Acta Universitatis Tamperensis | Insights into the use of adipose stem cells for clinical cell therapy: Novel culturing conditions and characterization of multipotency and immunogenic properties [Handle: 10024] |
| PLoS One | Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2, 3-Dioxygenase [pubmed:27695127] |
| Journal of Biomedical Nanotechnology | Exploring Amino Acid-Capped Nanoparticles for Selective Anti-Parasitic Action and Improved Host Biocompatibility [Pubmed:29883557] |
| Biochemistry | Mechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells [] |
| American Journal of Pathology | Cognitive Decline, Cerebral-Spleen Tryptophan Metabolism, Oxidative Stress, Cytokine Production, and Regulation of the Txnip Gene in 3xTg-AD Mice [Pubmed: 30980800] |
| international immunopharmacology | Interaction of the immune-inflammatory and the kynurenine pathways in rats resistant to antidepressant treatment in model of depression [Pubmed: 31176083] |
| Stem Cell Reports | Richard Schäfer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Kuçi, 4 [] |
| International Journal of?Tryptophan?Research | Altered Indoleamine 2, 3-Dioxygenase Production and Its Association to Inflammatory Cytokines in Peripheral Blood Mononuclear Cells Culture of Type 2 Diabetes?¡ [33343199] |
| Stem Cell Reports | Richard Sch?fer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Ku?i, 4 [] |
| Transl Psychiatry | Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive … [34802039] |
