血管非炎性蛋白1(VNN1)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Vanin 1 (VNN1)
Pantetheinase; Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1
- 编号KSC598Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, urine, tissue homogenates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.059ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7560 ¥ 12600 ¥ 22680 ¥ 44100 ¥ 75600
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对血管非炎性蛋白1(VNN1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPC598Hu02 | 血管非炎性蛋白1(VNN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC598Hu03 | 血管非炎性蛋白1(VNN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC598Hu01 | 血管非炎性蛋白1(VNN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC598Hu05 | 血管非炎性蛋白1(VNN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC598Hu04 | 血管非炎性蛋白1(VNN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAC598Hu02 | 血管非炎性蛋白1(VNN1)多克隆抗体 | WB; IHC; ICC; IP. |
PAC598Hu04 | 血管非炎性蛋白1(VNN1)多克隆抗体 | WB; IHC; ICC; IP. |
PAC598Hu03 | 血管非炎性蛋白1(VNN1)多克隆抗体 | WB |
PAC598Hu01 | 血管非炎性蛋白1(VNN1)多克隆抗体 | WB |
LAC598Hu71 | 血管非炎性蛋白1(VNN1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAC598Hu24 | 血管非炎性蛋白1(VNN1)单克隆抗体 | WB |
MAC598Hu21 | 血管非炎性蛋白1(VNN1)单克隆抗体 | WB |
MAC598Hu22 | 血管非炎性蛋白1(VNN1)单克隆抗体 | WB; IHC; ICC; IP. |
SEC598Hu | 血管非炎性蛋白1(VNN1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC598Hu | 血管非炎性蛋白1(VNN1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSC598Hu01 | 血管非炎性蛋白1(VNN1)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
Toxicology | Vanin-1; a potential biomarker for nephrotoxicant-induced renal injury [ScienceDirect: S0300483X11003234] |
Journal of Pharmacology and Experimental Therapeutics | Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury [Aspet: 192807] |
J Pharmacol Exp Ther | Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury. [PubMed: 22399813] |
Journal of Applied Toxicology | Early detection of renal injury using urinary vanin-1 in rats with experimental colitis [PubMed: 23307618] |
Iranian Journal of Health and Physical Activity | Effects of Exercise and Ferula Gummosa on Apelin of Cardiac and Kidney Tissues in L-NAME induced Hypertension in Rats [Jm.um: Source] |
Platelets | Value of vanin-1 assessment in adult patients with primary immune thrombocytopenia [Pubmed: 23534352] |
Journal of Applied Toxicology | Early detection of renal injury using urinary vanin-1 in rats with experimental colitis. [Pubmed: 23307618] |
Diabetes & Vascular Disease Research | Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats [Sagepub:Source] |
Food Chem Toxicol. | The effect of activated charcoal on adenine-induced chronic renal failure in rats [Pubmed:24412558] |
Hypertension Research | Early urinary biomarkers for renal tubular damage in spontaneously hypertensive rats on a high salt intake [PubMed: 26376947] |
Inflammation | Citrate Attenuates Adenine-Induced Chronic Renal Failure in Rats by Modulating the Th17/Treg Cell Balance [PubMed: 26253296] |
Toxicology | Early prediction of cisplatin-induced nephrotoxicity by urinary vanin-1 in patients with urothelial carcinoma [Pubmed:27317936] |
J Biomed Mater Res A | Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of mice [Pubmed:26850371] |
APMIS | Citrate attenuates vascular calcification in chronic renal failure rats. [pubmed:28332248] |
Clin Exp Pharmacol Physiol. | Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats. [pubmed:29027259] |
Cellular Physiology and Biochemistry | Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats … [Pubmed:29550811] |
International Journal of Molecular Sciences | Vanin-1 in Renal Pelvic Urine Reflects Kidney Injury in a Rat Model of Hydronephrosis [Pubmed: 30332759] |
International Journal of Molecular Sciences | Involvement of Vanin-1 in Ameliorating Effect of Oxidative Renal Tubular Injury in Dahl-Salt Sensitive Rats [Pubmed: 31514290] |
The Journal of Clinical Hypertension homepage | Urinary vanin‐1 associated with chronic kidney disease in hypertensive patients: A pilot study [Pubmed: 32869948] |
J Clin Hypertens | Association of urinary vanin©\1 with kidney function decline in hypertensive patients [34029440] |
Plasma vanin-1 as a novel biomarker of sepsis for trauma patients: a prospective multicenter cohort study [33624223] |
留言咨询