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血管非炎性蛋白1(VNN1)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Vanin 1 (VNN1) ,etc. by FLIA (Flow Luminescence Immunoassay)

Pantetheinase; Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1

（注：单次混测多因子不超过8个指标 ）

  • 血管非炎性蛋白1(VNN1)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 血管非炎性蛋白1(VNN1)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测血管非炎性蛋白1(VNN1)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的血管非炎性蛋白1(VNN1)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 92-101 96
EDTA plasma(n=5) 85-96 90
heparin plasma(n=5) 95-103 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的血管非炎性蛋白1(VNN1)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管非炎性蛋白1(VNN1)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 78-103% 92-105% 81-101% 94-102%
EDTA plasma(n=5) 96-104% 84-103% 80-90% 86-94%
heparin plasma(n=5) 83-105% 78-92% 87-101% 83-93%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将血管非炎性蛋白1(VNN1)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的血管非炎性蛋白1(VNN1)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的血管非炎性蛋白1(VNN1)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的血管非炎性蛋白1(VNN1)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPC598Hu02 血管非炎性蛋白1(VNN1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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RPC598Hu01 血管非炎性蛋白1(VNN1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC598Hu05 血管非炎性蛋白1(VNN1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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MAC598Hu22 血管非炎性蛋白1(VNN1)单克隆抗体 WB; IHC; ICC; IP.
SEC598Hu 血管非炎性蛋白1(VNN1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC598Hu 血管非炎性蛋白1(VNN1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
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参考文献

杂志 参考文献
Toxicology Vanin-1; a potential biomarker for nephrotoxicant-induced renal injury [ScienceDirect: S0300483X11003234]
Journal of Pharmacology and Experimental Therapeutics Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury [Aspet: 192807]
J Pharmacol Exp Ther Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury. [PubMed: 22399813]
Journal of Applied Toxicology Early detection of renal injury using urinary vanin-1 in rats with experimental colitis [PubMed: 23307618]
Iranian Journal of Health and Physical Activity Effects of Exercise and Ferula Gummosa on Apelin of Cardiac and Kidney Tissues in L-NAME induced Hypertension in Rats [Jm.um: Source]
Platelets Value of vanin-1 assessment in adult patients with primary immune thrombocytopenia [Pubmed: 23534352]
Journal of Applied Toxicology Early detection of renal injury using urinary vanin-1 in rats with experimental colitis. [Pubmed: 23307618]
Diabetes & Vascular Disease Research Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats [Sagepub:Source]
Food Chem Toxicol. The effect of activated charcoal on adenine-induced chronic renal failure in rats [Pubmed:24412558]
Hypertension Research Early urinary biomarkers for renal tubular damage in spontaneously hypertensive rats on a high salt intake [PubMed: 26376947]
Inflammation Citrate Attenuates Adenine-Induced Chronic Renal Failure in Rats by Modulating the Th17/Treg Cell Balance [PubMed: 26253296]
Toxicology Early prediction of cisplatin-induced nephrotoxicity by urinary vanin-1 in patients with urothelial carcinoma [Pubmed:27317936]
J Biomed Mater Res A Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of mice [Pubmed:26850371]
APMIS Citrate attenuates vascular calcification in chronic renal failure rats. [pubmed:28332248]
Clin Exp Pharmacol Physiol. Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats. [pubmed:29027259]
Cellular Physiology and Biochemistry Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats … [Pubmed:29550811]
International Journal of Molecular Sciences Vanin-1 in Renal Pelvic Urine Reflects Kidney Injury in a Rat Model of Hydronephrosis [Pubmed: 30332759]
International Journal of Molecular Sciences Involvement of Vanin-1 in Ameliorating Effect of Oxidative Renal Tubular Injury in Dahl-Salt Sensitive Rats [Pubmed: 31514290]
The Journal of Clinical Hypertension homepage Urinary vanin‐1 associated with chronic kidney disease in hypertensive patients: A pilot study [Pubmed: 32869948]
J Clin Hypertens Association of urinary vanin©\1 with kidney function decline in hypertensive patients [34029440]
Plasma vanin-1 as a novel biomarker of sepsis for trauma patients: a prospective multicenter cohort study [33624223]
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