血管非炎性蛋白1(VNN1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Vanin 1 (VNN1)
Pantetheinase; Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1
- 编号SEC598Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.156-10ng/mL
- 灵敏度最小可检测剂量小于等于0.059ng/mL.
- 样本类型serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2940 ¥ 4200 ¥ 18900 ¥ 35700 ¥ 294000
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测血管非炎性蛋白1(VNN1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的血管非炎性蛋白1(VNN1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 79-99 | 83 |
| EDTA plasma(n=5) | 80-94 | 89 |
| heparin plasma(n=5) | 82-94 | 91 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的血管非炎性蛋白1(VNN1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管非炎性蛋白1(VNN1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-97% | 96-105% | 84-94% | 95-103% |
| EDTA plasma(n=5) | 80-101% | 97-105% | 86-94% | 85-95% |
| heparin plasma(n=5) | 78-98% | 88-96% | 91-105% | 83-101% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将血管非炎性蛋白1(VNN1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的血管非炎性蛋白1(VNN1)与连接于固相载体上的抗体结合，然后加入生物素化的血管非炎性蛋白1(VNN1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管非炎性蛋白1(VNN1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC598Hu02 | 血管非炎性蛋白1(VNN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu03 | 血管非炎性蛋白1(VNN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu01 | 血管非炎性蛋白1(VNN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu05 | 血管非炎性蛋白1(VNN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu04 | 血管非炎性蛋白1(VNN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC598Hu02 | 血管非炎性蛋白1(VNN1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAC598Hu04 | 血管非炎性蛋白1(VNN1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAC598Hu03 | 血管非炎性蛋白1(VNN1)多克隆抗体 | WB |
| PAC598Hu01 | 血管非炎性蛋白1(VNN1)多克隆抗体 | WB |
| LAC598Hu71 | 血管非炎性蛋白1(VNN1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAC598Hu24 | 血管非炎性蛋白1(VNN1)单克隆抗体 | WB |
| MAC598Hu21 | 血管非炎性蛋白1(VNN1)单克隆抗体 | WB |
| MAC598Hu22 | 血管非炎性蛋白1(VNN1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEC598Hu | 血管非炎性蛋白1(VNN1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC598Hu | 血管非炎性蛋白1(VNN1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSC598Hu01 | 血管非炎性蛋白1(VNN1)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Toxicology | Vanin-1; a potential biomarker for nephrotoxicant-induced renal injury [ScienceDirect: S0300483X11003234] |
| Journal of Pharmacology and Experimental Therapeutics | Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury [Aspet: 192807] |
| J Pharmacol Exp Ther | Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury. [PubMed: 22399813] |
| Journal of Applied Toxicology | Early detection of renal injury using urinary vanin-1 in rats with experimental colitis [PubMed: 23307618] |
| Iranian Journal of Health and Physical Activity | Effects of Exercise and Ferula Gummosa on Apelin of Cardiac and Kidney Tissues in L-NAME induced Hypertension in Rats [Jm.um: Source] |
| Platelets | Value of vanin-1 assessment in adult patients with primary immune thrombocytopenia [Pubmed: 23534352] |
| Journal of Applied Toxicology | Early detection of renal injury using urinary vanin-1 in rats with experimental colitis. [Pubmed: 23307618] |
| Diabetes & Vascular Disease Research | Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats [Sagepub:Source] |
| Food Chem Toxicol. | The effect of activated charcoal on adenine-induced chronic renal failure in rats [Pubmed:24412558] |
| Hypertension Research | Early urinary biomarkers for renal tubular damage in spontaneously hypertensive rats on a high salt intake [PubMed: 26376947] |
| Inflammation | Citrate Attenuates Adenine-Induced Chronic Renal Failure in Rats by Modulating the Th17/Treg Cell Balance [PubMed: 26253296] |
| Toxicology | Early prediction of cisplatin-induced nephrotoxicity by urinary vanin-1 in patients with urothelial carcinoma [Pubmed:27317936] |
| J Biomed Mater Res A | Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of mice [Pubmed:26850371] |
| APMIS | Citrate attenuates vascular calcification in chronic renal failure rats. [pubmed:28332248] |
| Clin Exp Pharmacol Physiol. | Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats. [pubmed:29027259] |
| Cellular Physiology and Biochemistry | Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats … [Pubmed:29550811] |
| International Journal of Molecular Sciences | Vanin-1 in Renal Pelvic Urine Reflects Kidney Injury in a Rat Model of Hydronephrosis [Pubmed: 30332759] |
| International Journal of Molecular Sciences | Involvement of Vanin-1 in Ameliorating Effect of Oxidative Renal Tubular Injury in Dahl-Salt Sensitive Rats [Pubmed: 31514290] |
| The Journal of Clinical Hypertension homepage | Urinary vanin‐1 associated with chronic kidney disease in hypertensive patients: A pilot study [Pubmed: 32869948] |
| J Clin Hypertens | Association of urinary vanin©\1 with kidney function decline in hypertensive patients [34029440] |
| Plasma vanin-1 as a novel biomarker of sepsis for trauma patients: a prospective multicenter cohort study [33624223] |
