补体成分5a(C5a)单克隆抗体
Monoclonal Antibody to Complement Component 5a (C5a)
- 编号MAA388Ra22
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 来源单抗制备
- 宿主小鼠
- 效价(克隆号)C3
- Ig亚型 IgG2a Kappa
- 纯化方式蛋白A + 蛋白G亲和纯化
- 标记物无标记物
- 免疫原 RPA388Ra01-补体成分5a(C5a)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度1mg/mL
- 且适物种Rattus norvegicus (Rat，大鼠)
- 应用WB; IHC; ICC; IP.
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 666 ¥ 1554 ¥ 2220 ¥ 5550 ¥ 22200
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
Western Blot; Sample: Lane1: Rat Serum; Lane2: Rat Plasma Primary Ab: 3µg/ml Mouse Anti-Human C5a Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
-
通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对C5a的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别C5a。
用法
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| APA388Ra61 | 补体成分5a(C5a)活性蛋白 | Cell culture; Activity Assays. |
| EPA388Ra61 | 补体成分5a(C5a)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA388Ra01 | 补体成分5a(C5a)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA388Ra02 | 补体成分5a(C5a)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA388Ra01 | 补体成分5a(C5a)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA388Ra02 | 补体成分5a(C5a)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA388Ra06 | 补体成分5a(C5a)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA388Ra81 | 补体成分5a(C5a)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA388Ra71 | 补体成分5a(C5a)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA388Ra22 | 补体成分5a(C5a)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA388Ra23 | 补体成分5a(C5a)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA388Ra24 | 补体成分5a(C5a)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA388Ra25 | 补体成分5a(C5a)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA388Ra21 | 补体成分5a(C5a)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA388Ra | 补体成分5a(C5a)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA388Ra | 补体成分5a(C5a)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA388Ra | 补体成分5a(C5a)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Molecular Immunology | Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro [ScienceDirect: S0161589011008297] |
| Journal of Reproductive Immunology | Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia [PubMed: 23415845] |
| The Journal of Clinical Investigation | Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis [PubMed: PMC3195471] |
| Journal of Antimicrobial Chemotherapy | Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model [Pubmed: 23645587] |
| Journal of Orthopaedic Research | Conversion from external fixator to intramedullary nail causes a second hit and impairs fracture healing in a severe trauma model [Pubmed: 23070742] |
| SpringerPlus | Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats [Pubmed: 24353977] |
| American Journal fo Reproductive Immunology | Evaluation of the Markers of Inflammation in the Umbilical Cord Blood of Newborns of Mothers with Thrombophilia [Pubmed:25209155] |
| Gynecol Obstet Invest | Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? [PubMed: 26485247] |
| Journal of Neuroimmunology | Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis [Pubmed:27235358] |
| International Immunopharmacology | Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery [Pubmed:26802602] |
| Digital Repository | Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos [10183] |
| Environmental Pollution | Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia [pubmed:27511439] |
| Cancer Letters | Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expression [pubmed:29031586] |
| Acta Paediatrica | Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children [DOI: 10.1111/apa.14106] |
| Scientific Reports | Splenectomy modulates early immuno-inflammatory responses to trauma-hemorrhage and protects mice against secondary sepsis [Pubmed: 30291296] |
| Nutrients | The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes [Pubmed: 30736479] |
| Journal of NeuroVirology | Intrathecal complement activation by the classical pathway in tick-borne encephalitis [Pubmed: 30850976] |
| PLoS One | Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model [Pubmed: 31247004] |
| Frontiers in Immunology | C5a/C5aR Pathway is Critical for the Pathogenesis of Psoriasis [Pubmed: 31447855] |
| Phytomedicine | Novel evidence that an alternative complement cascade pathway is involved in optimal mobilization of hematopoietic stem/progenitor cells in Nlrp3 … [] |
| Aging | C5aR deficiency attenuates the breast cancer development via the p38/p21 axis [Pubmed: 32669478] |
| STEM CELL REVIEWS AND REPORTS | A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … [Pubmed: 32406006] |
| Modulation of C5a-C5aR interactions against murine mammary cancer cell line [] | |
| FASEB J | C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease [Pubmed:35503088] |
留言咨询