失调蛋白10(ATXN10)多克隆抗体
Polyclonal Antibody to Ataxin 10 (ATXN10)
E46L; SCA10; Spinocerebellar Ataxia 10; Brain protein E46 homolog
- 编号PAD248Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPD248Hu01-失调蛋白10(ATXN10)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度500µg/mL
- 且适物种Sus scrofa; Porcine (Pig，猪)
- 应用WB; IHC; ICC; IP; FCM
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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产品包装(模拟)
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产品包装(模拟)
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Figure: FITC staining on IHC-P; Sample: Hela cells.
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DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human ATXN10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human ATXN10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human ATXN10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human ATXN10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human ATXN10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 30µg/ml Rabbit Anti-Human ATXN10 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue
Primary Ab: 30µg/ml Rabbit Anti-Human ATXN10 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Figure: FITC staining on IHC-P;
Sample: Hela cells.
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Figure. Western Blot; Sample: Recombinant ATXN10, Human.
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Western Blot; Samples: Lane1: 293T cell lysate; Lane2: Hela cell lysate; Lane3: Porcine Kidney lysate; Lane4: Porcine Cerebrum lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human ATXN10 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human Hela cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human ATXN10 Polyclonal Antibody (Catalog PAD248Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对ATXN10的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别ATXN10。
用法
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPD248Hu01 | 失调蛋白10(ATXN10)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD248Hu01 | 失调蛋白10(ATXN10)多克隆抗体 | WB; IHC; ICC; IP; FCM |
| MAD248Hu21 | 失调蛋白10(ATXN10)单克隆抗体 | WB; IHC; ICC; IP. |
| SED248Hu | 失调蛋白10(ATXN10)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD248Hu | 失调蛋白10(ATXN10)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Molecular Metabolism | Ataxin-10 is part of a cachexokine cocktail triggering cardiac metabolic dysfunction in cancer cachexia [Science: Article] |
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