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脱氧核糖核酸酶Ⅰ(DNASE1)多克隆抗体

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human DNASE1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human DNASE1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human DNASE1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human DNASE1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant DNASE1, Human.
Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: DNASE1 knockout 293T cell lysate;
Predicted MW: 20,31kDa
Observed MW: 20kDa
Primary Ab: 2µg/ml Rabbit Anti-Human DNASE1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human DNASE1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Stomach lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human DNASE1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Colon lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human DNASE1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

该抗体是针对DNASE1的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别DNASE1。

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

杂志	参考文献
J Card Fail	Low Circulating Levels of Mitochondrial and High Levels of Nuclear DNA Predict Mortality in Chronic Heart Failure [Pubmed:27349571]
Biochimie	Inhibition of nuclease activity by a splice-switching oligonucleotide targeting deoxyribonuclease 1 mRNA prevents apoptosis progression and prolong viability of … [Pubmed: 32315661]
Scientific Reports	Biomarkers of neutrophil extracellular traps (NETs) and nitric oxide-(NO)-dependent oxidative stress in women who miscarried [Pubmed: 32753622]
J Am Heart Assoc	DNase 1 Protects From Increased Thrombin Generation and Venous Thrombosis During Aging: Cross‐Sectional Study in Mice and Humans [Pubmed:35023342]

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编号	适用物种：Homo sapiens (Human，人)	应用(仅供研究使用，不用于临床诊断！)
APB127Hu01	脱氧核糖核酸酶Ⅰ(DNASE1)活性蛋白	Cell culture; Activity Assays.
RPB127Hu01	脱氧核糖核酸酶Ⅰ(DNASE1)重组蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAB127Hu01	脱氧核糖核酸酶Ⅰ(DNASE1)多克隆抗体	WB; IHC
LAB127Hu81	脱氧核糖核酸酶Ⅰ(DNASE1)多克隆抗体(异硫氰酸荧光素标记)	WB; IHC; ICC; IF.
LAB127Hu71	脱氧核糖核酸酶Ⅰ(DNASE1)多克隆抗体(生物素标记)	WB; IHC; ICC.
MAB127Hu22	脱氧核糖核酸酶Ⅰ(DNASE1)单克隆抗体	WB,IHC
MAB127Hu21	脱氧核糖核酸酶Ⅰ(DNASE1)单克隆抗体	WB; IHC; ICC; IP.
LAB127Hu82	脱氧核糖核酸酶Ⅰ(DNASE1)单克隆抗体(异硫氰酸荧光素标记)	WB; IHC; ICC; IF.
LAB127Hu72	脱氧核糖核酸酶Ⅰ(DNASE1)单克隆抗体(生物素标记)	WB; IHC; ICC.
SEB127Hu	脱氧核糖核酸酶Ⅰ(DNASE1)检测试剂盒(酶联免疫吸附试验法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB127Hu	脱氧核糖核酸酶Ⅰ(DNASE1)等多因子检测试剂盒(流式荧光发光法)	FLIA Kit for Antigen Detection.