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非神经元性烯醇化酶(ENO1)多克隆抗体

Polyclonal Antibody to Enolase 1 (ENO1)

NNE; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding

  • 非神经元性烯醇化酶(ENO1)多克隆抗体 产品包装(模拟)
  • 非神经元性烯醇化酶(ENO1)多克隆抗体 产品包装(模拟)
  • 非神经元性烯醇化酶(ENO1)多克隆抗体 DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Mouse ENO1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • 非神经元性烯醇化酶(ENO1)多克隆抗体 DAB staining on IHC-P;
    Samples: Mouse Liver Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse NNE Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 非神经元性烯醇化酶(ENO1)多克隆抗体 DAB staining on IHC-P;
    Sample: Mouse Skeletal muscle Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Mouse ENO1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 非神经元性烯醇化酶(ENO1)多克隆抗体 DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse ENO1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 非神经元性烯醇化酶(ENO1)多克隆抗体 DAB staining on IHC-P;
    Sample: Mouse Skeletal muscle Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse ENO1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 非神经元性烯醇化酶(ENO1)多克隆抗体 FITC staining on IF;
    Samples: Human MCF7 cell;
    Primary Ab: 20μg/ml Rabbit Anti-Mouse NNE Antibody
    Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • 非神经元性烯醇化酶(ENO1)多克隆抗体 FITC staining on IF;
    Sample: MCF7 cell
    Primary Ab: 20µg/ml Rabbit Anti-Mouse ENO1 Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAB449Mu01.jpg Figure. Western Blot; Sample: Recombinant ENO1, Mouse.
  • 非神经元性烯醇化酶(ENO1)多克隆抗体 Knockout Varification:
    Lane 1: Wild-type MCF7 cell lysate;
    Lane 2: NNE knockout MCF7 cell lysate;
    Predicted MW: 36,47kDa
    Observed MW: 50kDa
    Primary Ab: 1µg/ml Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 非神经元性烯醇化酶(ENO1)多克隆抗体 Western Blot; Sample: Mouse Placenta lysate;
    Primary Ab: 2µg/mL Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 非神经元性烯醇化酶(ENO1)多克隆抗体 Western Blot; Sample: Rat Mammary gland lysate;
    Primary Ab: 2µg/mL Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 非神经元性烯醇化酶(ENO1)多克隆抗体 Western Blot; Sample: Human MCF7 cell lysate;
    Primary Ab: 2µg/mL Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 非神经元性烯醇化酶(ENO1)多克隆抗体 Western Blot; Sample: Mouse Colon lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 非神经元性烯醇化酶(ENO1)多克隆抗体 Western Blot; Sample: Mouse Kidney lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Mouse NNE Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对ENO1的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别ENO1。

用法

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

赠品

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPB449Mu01 非神经元性烯醇化酶(ENO1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB449Mu01 非神经元性烯醇化酶(ENO1)多克隆抗体 WB,IHC,ICC/IF
LAB449Mu71 非神经元性烯醇化酶(ENO1)多克隆抗体(生物素标记) WB; IHC; ICC.
LAB449Mu81 非神经元性烯醇化酶(ENO1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
SEB449Mu 非神经元性烯醇化酶(ENO1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB449Mu 非神经元性烯醇化酶(ENO1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB449Mu01 非神经元性烯醇化酶(ENO1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Analytical Chemistry Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor [PubMed: 20557064]
Translational Research Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules [Pubmed:27172385]
Journal of Proteomics Putative salivary biomarkers useful to differentiate patients with fibromyalgia [Pubmed:29654921]
BioMed Research International The Diagnostic Accuracy of Combined Enolase/Cr, CA125, and CA19-9 in the Detection of Endometriosis [Pubmed: 33062681]
Cancer Cell International Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins []
Cancer Cell Int Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212]
Clin Proteomics Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinoma [Pubmed:35610567]
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