生长因子受体结合蛋白10(Grb10)多克隆抗体
Polyclonal Antibody to Growth Factor Receptor Bound Protein 10 (Grb10)
GRB-IR; IRBP; MEG1; RSS; GRB10 adapter protein; Insulin receptor-binding protein Grb-IR
- 编号PAF468Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPF468Hu01-生长因子受体结合蛋白10(Grb10)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度0.5mg/ml
- 且适物种Mus musculus (Mouse，小鼠)
- 应用WB,IHC; FCM
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human Grb10 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Grb10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Grb10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Cerebrum Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Grb10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Grb10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Grb10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Cerebrum Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human Grb10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Uterus Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human Grb10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Liver Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human Grb10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human Grb10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Skeletal muscle Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human Grb10 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant Grb10, Human.
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Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Hela cell lysate; Lane3: Mouse Brain lysate
Primary Ab: 1ug/ml Rabbit Anti-Human Grb10 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: Grb10 knockout Hela cell lysate;
Predicted MW: 67,62kd
Observed MW: 65kd
Primary Ab: 1µg/ml Rabbit Anti-Human Grb10 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human K562 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human Grb10 Polyclonal Antibody (Catalog PAF468Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对Grb10的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别Grb10。
用法
Western blotting: 0.01-2μg/mL
Immunohistochemistry: 5-20μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPF468Hu01 | 生长因子受体结合蛋白10(Grb10)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF468Hu01 | 生长因子受体结合蛋白10(Grb10)多克隆抗体 | WB,IHC; FCM |
| MAF468Hu21 | 生长因子受体结合蛋白10(Grb10)单克隆抗体 | WB; IHC; ICC; IP. |
| SEF468Hu | 生长因子受体结合蛋白10(Grb10)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCF468Hu | 生长因子受体结合蛋白10(Grb10)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMF468Hu | 生长因子受体结合蛋白10(Grb10)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
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