60kD热休克蛋白(Hsp60)多克隆抗体
Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)
HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein
- 编号PAA822Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPA822Hu01-60kD热休克蛋白(Hsp60)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度0.5mg/ml
- 且适物种Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，猪), Bos taurus; Bovine (Cattle，牛), Chicken (Gallus，鸡)
- 应用WB,IHC,ICC/IF
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 569 ¥ 1327 ¥ 1896 ¥ 4740 ¥ 18960
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human HSPD1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Small intestine Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Spleen Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Small intestine Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Lymph node Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Lung Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Spinal cord Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Spleen Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Small intestine Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human Hsp60 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human Hsp60 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human Hsp60 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20ug/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 1μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
FITC staining on IF;Sample: MCF7 cellPrimary Ab: 20µg/ml Rabbit Anti-Human Hsp60 AntibodySecond Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: SAA544Rb11)
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AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant HSPD1, Human.
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Figure. Western Blot; Sample: Lane1: Rat Liver Tissue; Lane2: Rat Pancreas Tissue; Lane3: Human A549 Cells; Lane4: Human MCF-7 Cells.
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Knockout Varification:
Lane 1: Wild-type A549 cell lysate;
Lane 2: HSPD1 knockout A549 cell lysate;
Predicted MW: 17,61kd
Observed MW: 58kd
Primary Ab: 1µg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Porcine Liver lysate; Lane3: Bovine Liver lysate; Lane4: Gallus Liver lysate; Lane5: Canine Liver lysate
Primary Ab: 0.05μg/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对Hsp60的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别Hsp60。
用法
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
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| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA822Hu02 | 60kD热休克蛋白(Hsp60)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA822Hu01 | 60kD热休克蛋白(Hsp60)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA822Hu01 | 60kD热休克蛋白(Hsp60)多克隆抗体 | WB,IHC,ICC/IF |
| PAA822Hu02 | 60kD热休克蛋白(Hsp60)多克隆抗体 | WB; IHC; ICC; IP; FCM |
| FAA822Hu01 | 抗60kD热休克蛋白(Hsp60)多克隆抗体 | Flow cytometry. |
| LAA822Hu81 | 60kD热休克蛋白(Hsp60)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA822Hu71 | 60kD热休克蛋白(Hsp60)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| FAA822Hu41 | 抗60kD热休克蛋白(Hsp60)多克隆抗体(藻红素标记) | Flow cytometry. |
| MAA822Hu22 | 60kD热休克蛋白(Hsp60)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA822Hu21 | 60kD热休克蛋白(Hsp60)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA822Hu23 | 60kD热休克蛋白(Hsp60)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA822Hu24 | 60kD热休克蛋白(Hsp60)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA822Hu25 | 60kD热休克蛋白(Hsp60)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA822Hu27 | 60kD热休克蛋白(Hsp60)单克隆抗体 | WB; IHC; ICC; IP. |
| FAA822Hu02 | 抗60kD热休克蛋白(Hsp60)单克隆抗体 | Flow cytometry. |
| SEA822Hu | 60kD热休克蛋白(Hsp60)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA822Hu | 60kD热休克蛋白(Hsp60)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA822Hu | 60kD热休克蛋白(Hsp60)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA822Hu01 | 60kD热休克蛋白(Hsp60)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It(ELISA Kit) Yourself" |
参考文献
| 杂志 | 参考文献 |
| Sci Rep | Hsp60 response in experimental and human temporal lobe epilepsy [PubMed: 25801186] |
| Cellular and Molecular Gastroenterology and Hepatology | Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitro [Pubmed: 30878663] |
| Breast Cancer Res Treat | Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancer [Pubmed: 32601970] |
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